LOCUS Exported 5442 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: C1 Cloning Vector, Excitation: 554, Emission: 581. ACCESSION . VERSION . KEYWORDS tdTomato-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5442) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 5442) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5442 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 16..319 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 320..523 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 474..494 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 562..571 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 568..1995 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" misc_feature 1996..2061 /label=MCS /note="multiple cloning site" polyA_signal 2185..2306 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2222..2241) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2276..2295 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2313..2768) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2450..2471) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2662..2681 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2795..2899 /gene="bla" /label=AmpR promoter promoter 2901..3258 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2924..2944) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3109..3244 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3171..3190 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3293..4087 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3347..3366) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3957..3976 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4275..4294) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4319..4366 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4695..5283 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5184..5203 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca 541 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag 601 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag 661 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 721 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag 781 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc 841 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac 901 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc 961 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg 1021 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc 1081 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc 1141 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc 1201 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc 1261 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc 1321 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag 1381 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 1441 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc 1501 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc 1561 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc 1621 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac 1681 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag 1741 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag 1801 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa 1861 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac 1921 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg 1981 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac 2041 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca 2101 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 2161 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 2221 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 2281 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa 2341 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 2401 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 2461 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 2521 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 2581 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 2641 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 2701 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 2761 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 2821 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 2881 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 2941 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3001 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 3061 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 3121 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 3181 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 3241 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 3301 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 3361 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 3421 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 3481 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 3541 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 3601 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 3661 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 3721 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 3781 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 3841 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 3901 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 3961 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 4021 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 4081 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 4141 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4201 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 4261 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 4321 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 4381 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 4441 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 4501 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 4561 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 4621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 4681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 4741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 4801 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 4861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 4921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 4981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 5041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 5101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 5161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 5221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 5281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 5341 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag 5401 ttattaatag taatcaatta cggggtcatt agttcatagc cc //