LOCUS Exported 11596 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish. ACCESSION . VERSION . KEYWORDS p5E-elavl3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11596) TITLE Bonkowsky lab FingR plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 11596) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11596 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 593..688 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 690..708 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 9519..9535 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 9520..9536 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9570..9586) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9570..9586) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(9623..9641) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind 9641..9765 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(9838..9856) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9861..9877) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9861..9877) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9979..9998) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 9990..10799 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(10057..10076) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 10946..11534 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11435..11454 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg 601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta 721 ccgggccccc cctcgacgaa ttcccggccg cgtaccgggc cccccctcga aattcactaa 781 tttgaattta aatgcattat ctttctattc ctaaagacct tgggtgacca aaatcttatt 841 ttaataaata aaactgttta ttaaaacttt tttgtttcaa agaaccatat gtatagtgaa 901 atttataaaa atatcaattt ttaaaaagct ggtgtactca tttatgttat gaactctaaa 961 accatatact gactgcaagt gatgatgtat agagtgatgt ttacgagtaa acatatttag 1021 ttgtatacat cctactgagc acattttgat gtatgaaata acattacaag ctttacccaa 1081 attaagccat tttaaaacac tgccaattga aaatacaaat cctggaaaaa atcgtcttta 1141 gcgcagtcat ttgagccatc ctaatccgtt acctcagacc ataataagaa gggataacac 1201 tagctgtagc aatggaacac atctgtttca cacaatcata tctcctgcgc cggtgctaag 1261 cagattcagc gtgatcataa catgctttcc actcataaat gtaaatttac aatttgcaca 1321 tgtaaaacag acacttttga gatattggat aaaaaaacaa gagtatattg cttagtttca 1381 tccaccagtc atccccacag cgtttggaag gccataaaaa gtgtctaaaa tcaatgatca 1441 ttgaaagagc acaagagaga ctcttacgct gtaatgccac tggggacaaa agtgacagtc 1501 tcttaatggg ctcttctgga ggggctcctg aacattaaaa attatcagcg aaattaccga 1561 aagagcttca agcaactggc atgcttgatc ctctgcgtcg gggcggtgaa taggtgcttc 1621 agatgccctc ttacccacgg gctggattca gctgccccgc taccagcgga gaccccctaa 1681 tgagcctctg caattaagtt tattcatgtt aagtgtgaac ggggtgcgtg cggaactgtg 1741 ggcagctaac agacctgggt tctttgtgcc acaagtgctg cctttattcg gctcacaaag 1801 cagaaaacaa cacccgcacc tataatggcg ccctcggctg ggtctaagaa acgtggcgag 1861 ttgacagagc agagtgggcg gggttaagac agactgacag cgggacccat ctccatcctc 1921 ttattaacgc ttaacgagtg ccttcctcat gcaatattca tcgccactaa tatcatccaa 1981 gctctgagct gagctggcca cttatgtaag gcaattatgt aaaatatcag acagggccca 2041 cactcagaat ctgactgggg tagagacgcg ggacgagaac cgagagcaag aactgaaagt 2101 gaaagtgacc actaaaggga ggagaggaca gaggggcagg atgtgtcaag attaccagag 2161 aacacttggc cagaaatgcg caaccattgg agctctccgg attacccaaa ggttaacgag 2221 tttgaacgcc tctgcccact cgccatctct gatggtttcc caagaactcc tcaagcaaaa 2281 tatatataat tgtgtgtatt atgcacagac acgagaaaat gctgtttttc tgatctgcat 2341 tacagcacat ttgcccgcca acgacaatac cacccactcg gtacctcgct gactcctgat 2401 gcctgatacc tgcgcggtga ctgtctacaa tctgcataat caagagaagt tgtgttgaag 2461 acgagcgcca cacaaccgtt tccacaaggt cacccaaggc cggtgcagat gtaggtgagg 2521 tctccataaa cagactgaaa taaacacatc ctccgctggg aacaacaacc ccctcacgcc 2581 tcatgcattc cataagccta catgcatctc ttccaactta tggagactcg cacctaccaa 2641 catccgcaca acaaagatat acagagcgcg ctccctcagg tcaaggcctg tgggggtctg 2701 tgcagaaata ggtcatttgt cacacatcaa gtcctggggc aggagatgca ttatagatga 2761 gaccaaacag cctgtctcgg tgagctctac ccactccctg agactagaaa tgggggaagg 2821 gagcttgaga taacaaccgc tgcaatcact gtgtcgatgt taatatcagc accaaccggg 2881 aacaataagg agatggatgc attcatgttc acatcttacc agtcaagtat catcgaaccg 2941 gcttgataac cacacctcgt gtaatagctg agcagatagt tgtcatttta aagcgttggc 3001 ctttgtcgat tatgtaatgc gcacattcaa cacatggtaa tatagaaacg gttatgtcga 3061 ggttgttttg tccagagatg accttcacac agttacagcc gctctgcatc cacacaaatg 3121 gaggacttaa tcgtggactg cattcttaga aatgatctac aaagacaaat aatgtgaaat 3181 caagaaagga caaaatttaa gtaaggggat gagggagaga gagaacgagg ggcaaggaga 3241 aagcatggct cctgtctttt tctgcaccca tctgttcgga gtgcaggtgg agctctattc 3301 actcagctct gcatgtgtgt ttgggggggg caggaagaaa gggagggcaa aaggaagagt 3361 ggagagatgg tgggggctgg agggatgggg ggttctcggt gatctctcct gaaggggata 3421 atgggagagc agcgctttgc aatggctgcc atgtagtacc ctccctgcac aattagccaa 3481 tcagcagcaa ctctgccagc cagaaggaca cataaagaag aacattgcag cagaggcaca 3541 gaaggagcct gcgaggagct gggaaataca cacacaacag cagaaccaca acaccctccc 3601 ctggacacac cctactgggg atcactgctt ttcttttttt ctgaaccatc gcccacgcca 3661 cacggagaga aatctctctc tcatcatcat cctgaagaaa acccccttat cctcattttc 3721 acactgctga ggaaaaccta caatcgcacg ggctgagatt tcctggcgaa gactgtcctt 3781 tttccttttt cttttttttt cttttccttt ggaaactgac atttgcattt ctccattcca 3841 agccacggcg taataatatc tgcaatccag cctgaagacc tgcaaatcga aggacctaga 3901 tcactatcat ctttgtacgt caagagttac tgtacgtata acctttcttt cttttgctct 3961 gaccaatatg aaacactaaa atcgattcga gcagcctctc agcatcaatt acagtgcgtg 4021 aaaaacattc aaatagaggc agcaaatatt acatgtgaaa atacaggctg gctaaatcca 4081 agctaattta gaaatgtggt caaaacgcat actggcacgt ctaatcgcgg attcagtaaa 4141 caagattaac gattagccca gtgtataagt catatgatac aggcatgcgc gagagcatcg 4201 ctacacccga gctggcttca ttttcggagg aaaatcaaaa cattgctttc tcctgccgtg 4261 cgaaccattc gtcataaacc gtaatacgca acatacattt attactacat ccgttaatta 4321 gcgataatta gccgttatta acaaagagcg ctgaggaatt cctctcaaat agcggaggtg 4381 cggcggaggc agaggggcgt aaaagggcac atgcgtgcct ggctcaaaaa aggatgctcc 4441 agactgaaac tcagagacaa aacacgatgc ctcggaggct gagagcccat cgagaggaaa 4501 ggcaaagaaa agggtcgagt cactcagagg gggaggggag atattgtgca tccattggtt 4561 tgaattgaag caggcagaat aaaaatcctt gcgattatgc ttcgtgagag atcgcgagag 4621 aggaaaaggc taaatgccac gtatgcaaat ggataaatgt catctatttc ttcgcgagta 4681 gacattttgt ggacaacgag agtgtaaata agcgatatga ggggaataca aaggccttga 4741 gggaaagcgt tttgggcgtg tcttgtcgaa aagaagacag cgatggcacg cgctcagaac 4801 caccatcttc acgtagataa tccgcgtgaa aacaataaca aaactgctca ttttaacacg 4861 agcaggtgtt tgaaaccacc caaaaaagat tgtctaaaat attaagatta attaatgacg 4921 tttaaatgtc caggcctgat ataaatgaag cgtgtagtaa gagtaatcgc ctttgtttct 4981 tttcagagat acgttttcag agattcaggg aattatatct tcaataaaat aatgtttgat 5041 ttcattattt taaagctttt taagaagtaa aaatacacat tattcattta cattttattt 5101 tatttaaagg cctatggtct tgaaaataat aagtggaata tataggctat atattcgtct 5161 aaggacagcc ggtgttattt tatagtgacc tcagacaaaa gccgagacaa aacgaggcgc 5221 ttgcctttca ttttaatcct taatagacgg tgtgatgaat gaatgaatga atgaataaat 5281 gaatgaatga cgggcccttc ggcatgttgt tccgcagtcc tccgtgagca cgagtctcta 5341 ccgcagtgcc aagcacataa aaccgttgag tcagataacc ttctccaggc gcgtatacac 5401 aattcaagga caagcacatc acaactatac acaacacaac acaaccgcct aaacgcacga 5461 gctcatcaat cgcaatttaa aggcatgtca gtcaaagaaa aaggcggcgt tatttaaaac 5521 caatgttcaa caatatttgt atgctgacta gcccgggcgc gcacgctata agatcacaag 5581 gccttgtttt ttccctttat tcaaaatatc attaattatt acaggtcata aaacaaaaca 5641 atgcaaatta caatgattta aagactattg atttaacaat aatcagttaa ttatagaata 5701 atattataat attttacata caaatgaaag atgacattat ttgatgacat tgtaattaaa 5761 atatatgcaa tatattttta cgtttttatt aattaggcta ttactattat tataattatc 5821 attattttta ttattattat tattattaaa tagtcttgcg aaataagagt gaaggcagtt 5881 gcaatgttca ttgttcccgg agagtcccgc agcctctggc ctcaacacaa agcgctactg 5941 tttacattaa tattcatagt gctgccatgg tctctgtaga ggaggaacgg gtggctcttt 6001 tgttcgctag tgtcaatacg tttattcagc aaaaagcagc tcggatttgt gaatgcccaa 6061 caagcacacg cgcactccaa tcaaaggacg agaggataac gattttaaac gattcgcaaa 6121 cgtgtttatt tgtaaagacg acatcaagag cttaatgtcc acttgaaaag aaataaatat 6181 gcccgcggcg ccaaggatat tttgaatgta gtcgttcatg gtgagccact cccgaattca 6241 gcactctgga gagcgaccaa agaggaatag cggaatagca actacttgag ccatttctct 6301 ccgtttgatg gcgttcatga ttaaaaatat aatcacgtga ttttttatac atatataaat 6361 atatacataa agtaacgctt tccttttgag aatacataaa tatttattcg cgtgataaat 6421 caatgatctt atatttattt gcacgaccga aattagccaa attcaccaat taaaaaaaaa 6481 ataagccaac aaaaaagaag ggtttatccg ttcagttttg acttgtgtcc tgttgtttta 6541 cagctgccac tgtgatccct taagctgcag tgagagtgtg gctaatgcct tttgtttaag 6601 ataatcctgt aatctgttac cgaaacggcc tattgacaag ccggcattca catttcagtc 6661 aggaggcccg tccagacatg tacatttatg aattacgaat gataaaatta agatctgcat 6721 taggcgtcag aaatgtcacg aacacccatt catttcccac agccaataat ggatcatgct 6781 gggaagcgct atcctcgcag tctcaaaatt aatgttgaca tgttgcgtag agctacagat 6841 tagatcagcc atatgcttta tgtgtttacc tcagcggatc ttcagcaaga tgtgtttatt 6901 ttaagaaaat ggcttcctcg ctgcctgcac aggggcatta aggaagtgac gtgagcgtcc 6961 tcacagtcaa attactttat ctcatgctgc tttcaggcct ctgcttattt tattattatt 7021 attttaaatt agggggaatc acggtggcgt tgtgggtagg gtagcgcgat caccttacag 7081 caagaaggtc gctggttcga gctctggctg ggtcagttgg catttctgtg tggagtttaa 7141 attgaattga aattgaataa actaaattgg ccctagtgta tgtgtgtgaa tgtaagtgtg 7201 catgggtgtt tcccagtgtt gggtttgact ggaagagcat ccgcttcgta aaacatatgc 7261 tggataagtt ggcggttcat ttcactgtgg tgaccgctga ttaataaagg gactaagctg 7321 aaaatgaaat gaattaatac attttaaatg agcttttgct gacatgttta tgattacaaa 7381 tagtgaaatt gtatgttaat ttacgataag tgcattgttc taaattcatt tttatgtagc 7441 agctggtttt tcttccattt gtgtcattca acagaacatt tttctaaaaa agtgaactta 7501 aattttctcc tccctctgac aaggcttgat ggtaatttca gccgactcta attacagact 7561 cactaaaagg aatcggtgca gtcatttatc tttatcagcg cgctggtggg aaggtaatag 7621 gttttgctta tgagttgttt gcgtgcgagt ctgggtcctt gtgtaggcga tgctgatgca 7681 ctgcattaaa tattgaggga gaagtcgctc aaaaatagca aagtacaggc tggggcagga 7741 acgtcaaagc ccgctcagtg gccacgtttc ttgtttcgta agtcctattt ttaatagcga 7801 taattcaggc tcagtagaag gaactccagc actataagca gtctctcgcc tgtctcggcg 7861 agttatgtat tttgggatga agtgctcttc cacctctacc tctgctgtgc tggcgccagt 7921 atctgggctg taagatagtg tacgtgtcag tctgtgtggc cgaagagcct gtgtgttgtg 7981 taactcacta cattcgtcgc tgtggatggc tgacttgcca tggggaggtt tacttcagtt 8041 cacgaatcac attcgccacg atcttgtcac tttgtgtcaa gactcgctct ctgtgtgtca 8101 acagctctgc tgtttgaggt tgaaattctg aacttgagat atatcccagg atatattagg 8161 gctactgcca acggaaaaag caaatggtgc catggccatt tgattgatca aacaaacacc 8221 cttttgcttg ggtgacagtg aaatgtcaga tccatgtttg tactgcttta ataacttgcc 8281 tctctgagct ttctccttgt cagtgtcaat ttgcctgaga tataaattcc ggttgccccg 8341 aaagcttatt ttattaaaaa aaagctaaca tgcactcata caacgtgcgc acatacatgt 8401 gaggcttgct aaaagtcaag aagcaagcca atagcaagag tcgtcagact gctgttacct 8461 gggcaacctg gttagaggta aggctcaagg caagaataaa tcattccact gaagagacac 8521 agcgttacag cgagatgaga gcaagaggcg atagtgaagg gagaacaaaa gaacagccta 8581 cccacactag tgcagatcta cctgtttgag ctactgattg ttcagtttga ttagatatca 8641 gacaattctg ctatctccaa ttgcattgct gcatgaattc ctagaaatca ttttaatcac 8701 caaaaactag tagggacact tgtggcgtat atgattctgc ctgttgattg tggggatcca 8761 acagaaagat catgaatgca ttgttaatta agtcggtgag acaccctgtt ccaccctagg 8821 ggccgttctg gggaaagagt gctttcagtc agctaaagat gactaatatg tgaacatata 8881 ttactacaca tgccaatttg tacattctgg atagttacca gccctgggaa aacatcgaaa 8941 aaaaataaat caacatagat aaaattggca aatcccttct ggtacgatgc agagtgcttg 9001 ctctgagaac ttgtgcattg tgacagaaag cgaccgttaa aaactgaaag tagcatctac 9061 aacctgcata catctgttct ttgaaaatcc cctgtaggcc aaattaagca tcacgctgtg 9121 ctccacagca acacggatga accgaaaacc cagacacaaa cgcacacact tttcacataa 9181 acaaggttta gttatgctag ctatgctttt tcgtcctccc tttgtcatca gcggcgagtg 9241 acgtaacaca gtttgaccct ggacagcgta aagggggggt tgggaatgag tgaaaggact 9301 gtgacatctg cagggccagg ggctgatgga gagagctgaa gtgagggagg tcagaggagg 9361 aggaagggtg ggatattgtc ctcctcaggc cctatccctc tcagacgcat tgatttttaa 9421 cctctcagcg agaatgccaa gcattacacc cctcaaatta tggttcctca cccaacctgt 9481 tatattttcc acctgcagat aattagcacc attgaaactc gaggtcgacg gtatcgataa 9541 gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc 9601 ggtggagctc cagcttttgt tccctttagt gagggttaat tcaagtttgt acaaaaaagt 9661 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa 9721 acagactaca taatactgta aaacacaaca tatgcagtca ctatgaatca actacttaga 9781 tggtattagt gacctgtaga attcgagctc tagagctgca gggcggccgc gatatcccct 9841 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa 9901 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg 9961 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc 10021 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg 10081 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt 10141 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa 10201 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg 10261 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat 10321 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt 10381 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc 10441 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct 10501 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag 10561 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag 10621 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat 10681 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta 10741 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat 10801 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg 10861 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc 10921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 10981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 11041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 11101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 11161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 11221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 11281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt 11341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 11401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 11461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 11521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 11581 cttttgctca catgtt //