LOCUS       Exported                 944 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  (control) when used to produce lentivirus, express physiological 
            levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    MEK1-pcw107-V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(48..89)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcggtc attactacgt agaatcgaga
       61 ccgaggagag ggttagggat aggcttacca ccggtaacca ctttgtacaa gaaagttggc
      121 aagacgccag cagcatgggt tggtgtgctg ggctggttaa ggccgatggt ggagcagagc
      181 caacctgcaa aatccacttc ctcagcatca gatctcttga taaaagcatg aaccatgagt
      241 tgcttcaaat ctgctctctc tgcggggttt tttattaagc atttattcac aaaatcttga
      301 aattccagac tgaacactcc actgggcagt tttggaggag gctcgttgac tatgtaatcc
      361 aacaactcaa aaattgccat gggaggtcgg ctgtccattc catatgagct aaggggcctc
      421 ccgggggtcc ttggcctggg tggggtctca gccgcatctc cttccacctg gcacccaaac
      481 atcagctcca gctccttggc atctggagga gggatgggat acctcccaac cgccatctct
      541 accagagaca gtcccatgct ccagatgtct gactgcacag agtaatgagt cccctggagt
      601 ctttctggcg acatgtagga ccttgtgccc acgaaggagt tggccatgga gtcgatgagc
      661 tgcccgctga ccccaaagtc acagagcttg atctccccac gggagttgac taggatgttg
      721 gagggcttga catctctgtg catgatcttg tgcttctcca tcagatatgt caggcctttt
      781 attacagcaa tgctaacttt tcctaaaatt tgttcaggaa ttcttccagc tttcttcagg
      841 acttgatcca gagaacctcc atccatgtgc tccatgcaga tactgatctc gccatcgctg
      901 tagaacgcac catagaagcc cacgatgtac ggagagttgc actc
//
