LOCUS Exported 1000 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION AAV expression of ChR2(H134R)-GFP under the Syn promoter. ACCESSION . VERSION . KEYWORDS pAAV-Syn-ChR2(H134R)-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1000) AUTHORS Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K TITLE Millisecond-timescale, genetically targeted optical control of neural activity. JOURNAL Nat Neurosci. 2005 Sep . 8(9):1263-8. PUBMED 16116447 REFERENCE 2 (bases 1 to 1000) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1000 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(38..964) /codon_start=1 /product="H134R mutant of an N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2005)" /label=ChR2(H134R) /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGAV" regulatory 961..970 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 gctcctcgcc cttgctcact gctactaccg gtgccgccac ggctccggcc tcggcttcgt 61 cttcgacgag agtctcgacc tcgatctccg ttccgccgat gttcagtttg gtggttttgc 121 ggatatctcc gtgaatcaat atgtgctcgt ggatcaggac gcgcaggtag tgtcccaaca 181 acccccaaca atttttactc atcagatcaa taatcgtgtg acctacggtg gagccataga 241 cgctcaggac gccaaaacct tcgggcccca aaatgaagag aattgggaac ataccccagc 301 tcacgaaaaa cagccatgcc atgccggtca cgacctggcg gcaccgaccc tttggcacag 361 tatgataacc ctcgatatat gctttggcgg cgtgaaaaaa tgtgttcgcg ccatagcaca 421 atccaagaca aaagaagatg actttaacat agccggttgc catggcgctg gtagcccccc 481 acacgatagt cccgatgtct gagacaagga gtcccatggt tctcctgctg tagtcgttgc 541 tcaggccggt gaggttgctc agtctgataa ggatgacagg acaagtgagc agccactctg 601 catagcgcag ccactgcacc cggtgtcctg tggcaaggta gagcatagag ggattcttaa 661 actcaaaaaa gaactcgaga atcaccttaa ccatttcaat ggcgcacaca tagatctcct 721 cccagccgca tgtagatttc caggtttggt aggcatagaa catcagcagc aaaatgctga 781 atcctgctgc aagccactgc aggacatttg acgcggtctg agcgccgttc gtgccgcgag 841 attcaatcca tccggcacag taacattgat cctcagggac caggacggac ccgttcacca 901 ccacaggatt agtaacgaac aaaagttcgc gtccgacggc agacaaagcg ccgccatagt 961 ccatggtggc ggatcctctc gactgcgctc tcaggcacga //