LOCUS Exported 887 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Co-expresses HA tagged channelrhodopsin and hM4D from a Cre-dependent virus. ACCESSION . VERSION . KEYWORDS AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 887) AUTHORS Stachniak TJ, Ghosh A, Sternson SM TITLE Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus-->midbrain pathway for feeding behavior. JOURNAL Neuron. 2014 May 21;82(4):797-808. doi: 10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24. PUBMED 24768300 REFERENCE 2 (bases 1 to 887) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..887 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(65..98) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(169..202) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." regulatory 209..218 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcgaa cctcttcgag 61 ggacataact tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatctggac 121 tagagggtcg atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat 181 aaagtatcct atacgaagtt atactagtgc caccatggac tatggcggcg ctttgtctgc 241 cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt 301 ccctgaggat caatgttact gtgccggatg gattgaatct cgcggcacga acggcgctca 361 gaccgcgtca aatgtcctgc agtggcttgc agcaggattc agcattttgc tgctgatgtt 421 ctatgcctac caaacctgga aatctacatg cggctgggag gagatctatg tgtgcgccat 481 tgaaatggtt aaggtgattc tcgagttctt ttttgagttt aagaatccct ctatgctcta 541 ccttgccaca ggacaccggg tgcagtggct gcgctatgca gagtggctgc tcacttgtcc 601 tgtcatcctt atccgcctga gcaacctcac cggcctgagc aacgactaca gcaggagaac 661 catgggactc cttgtctcag acatcgggac tatcgtgtgg ggggctacca gcgccatggc 721 aaccggctat gttaaagtca tcttcttttg tcttggattg tgctatggcg cgaacacatt 781 ttttcacgcc gccaaagcat atatcgaggg ttatcatact gtgccaaagg gtcggtgccg 841 ccaggtcgtg accggcatgg catggctgtt tttcgtgagc tggggta //