LOCUS       Exported                 388 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-dCas9-p300 Core (D1399Y)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..159
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             166..186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgcc atggactaca aagaccatga cggtgattat
      121 aaagatcatg acatcgatta caaggatgac gatgacaaga tggcccccaa gaagaagagg
      181 aaggtgggcc gcggaatgga taagaaatac tcaataggct tagctatcgg cacaaatagc
      241 gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg
      301 ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt
      361 ggagagacag cggaagcgac tcgtctca
//
