LOCUS Exported 12126 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]. ACCESSION . VERSION . KEYWORDS pAWp7-19 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12126) AUTHORS Wong AS, Choi GC, Cheng AA, Purcell O, Lu TK TITLE Massively parallel high-order combinatorial genetics in human cells. JOURNAL Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. PUBMED 26280411 REFERENCE 2 (bases 1 to 12126) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12126 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 20..608 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(73..93) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(491..502) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(611..627) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(612..628) /label=pBluescriptKS /note="For pBluescript vector" LTR 1133..1313 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(1339..1356) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1345..1569 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1615..2043 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1702..1721) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1912..1933 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2052..2072) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2057..2386 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2237..2372 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2299..2318 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 2434..2481 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 2500..2874 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 3004..3125 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3041..3060) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3095..3114 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3174..3190) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3174..3190) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3187..3209) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3198..3214 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3222..3252) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3267..3288 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3405..3422) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3576..4164) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3656..3675) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4335..5195) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4958..4977 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5196..5300) /gene="bla" /label=AmpR promoter primer_bind complement(5375..5394) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 5566..5945 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5947..6145 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 6163..6343 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 6390..6515 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7008..7241 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7426..7470 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 7768..7885 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7939..9150 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 8987..9006 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 9167..9862 /codon_start=1 /product="red fluorescent protein from Entacmaea quadricolor" /label=TurboRFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC DLPSKLGHR" enhancer 9983..10362 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 10364..10562 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 10585..10594 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 10591..11310 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 10591..11307 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(10636..10657) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(10897..10916) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 11244..11265 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg 61 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 121 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 181 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 241 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 301 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 361 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc 421 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 481 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 541 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 601 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag 661 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 721 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 781 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 841 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa 901 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt 961 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca 1021 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga 1081 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct 1141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 1201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 1261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg 1321 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 1381 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 1441 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 1501 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 1561 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 1621 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 1681 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 1741 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 1801 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 1861 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 1921 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 1981 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 2041 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 2101 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 2161 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 2221 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 2281 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 2341 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 2401 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat 2461 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc 2521 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc 2581 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc 2641 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg 2701 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg 2761 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc 2821 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg 2881 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 2941 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 3001 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3121 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 3181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 3241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 3301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 3361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 3421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 4021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 4081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 4141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 4201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 4261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 4321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 4381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 4441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 4501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 4561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 4621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 4681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 4741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 4801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 4861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 4921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 4981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 5041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 5101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 5161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 5221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 5281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 5341 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt 5401 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa 5461 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta 5521 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga 5581 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 5641 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 5701 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 5761 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 5821 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 5881 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 5941 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 6001 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 6061 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 6121 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac 6181 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6241 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6301 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg 6361 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag 6421 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg 6481 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat 6541 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata 6601 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat 6661 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag 6721 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga 6781 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca 6841 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg 6901 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc 6961 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc 7021 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta 7081 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt 7141 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga 7201 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct 7261 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg 7321 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca 7381 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa 7441 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg 7501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt 7561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc 7621 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag 7681 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg 7741 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag 7801 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa 7861 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg 7921 gttaattaag ggtgcagcgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc 7981 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg 8041 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc 8101 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag 8161 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt 8221 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt 8281 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga 8341 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga 8401 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac 8461 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc 8521 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac 8581 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg 8641 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc 8701 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc 8761 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc 8821 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta 8881 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg 8941 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt 9001 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa 9061 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa 9121 ttctggccgt ttttggcttt tttgttagac agttaattaa gccaccatga gcgagctgat 9181 caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa 9241 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt 9301 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg 9361 cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc 9421 tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac 9481 ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa 9541 cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga 9601 gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt 9661 gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa 9721 gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc 9781 cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc 9841 tagcaaactg gggcacagat aataacctgc aaaaaggcat tcaccgcgtg ccttaggcat 9901 tcaccgcgtg ccttaggcat tcaccgcgtg ccttaggcat tcaccgcgtg ccttaaatgc 9961 aggcgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt 10021 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 10081 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 10141 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 10201 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 10261 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 10321 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 10381 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 10441 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 10501 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 10561 agcggtaacc cccgggtacc ggtcgccacc atggtgagca agggcgagga gctgttcacc 10621 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 10681 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 10741 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 10801 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 10861 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 10921 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 10981 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 11041 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 11101 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 11161 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 11221 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 11281 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagaga tctcatgcat 11341 cggatctaga gaggaggatc cgcgaaaagt ccggaattca ggtgggagta ctgctcagag 11401 ctacaactct aggagtaggg actccaagcc tagagctcca agagagggtg aagggcaggg 11461 agaaaattat agtaatagtt gcaatgagtc acttgcttct agatcaagat cagagactct 11521 gctctccgtg ttcacagcgg accttgattt aatgtcatac aattaaggca cgcggtgaat 11581 gccaagagcg gagcctacag ctgcacttga aggacatccg agagaagtta ggaagggtgg 11641 ggagaaacaa ttctagaatg aacccatcct gtgcgacacc aattcatact gtgaagtaca 11701 ctgcatataa ggagtgtggt atagtataaa gaaactttct gcaggtagta attatagtga 11761 agattttagg tttacaaagc cctagctgtt ttctgtgtag cttttattat tcttatgact 11821 cttgacaagt ttgtagcttc accatataca tttaatattt tgcaataatt ggccttgttc 11881 ctgagctgtt ggattcgggg ccgtagcact gtctgagagg tttacatttc tcacagtgaa 11941 ccggtctctt tttcagctgc ttcctggctt ctttttactc aggtttccac tgcttttttg 12001 ctttttttaa tgctgtatga aggtgttaac atttgtttat atttttcatt aattgtaata 12061 cctttaaatc atgcatcata ctcagaaata gggattagaa tttaagtgac atctttggcc 12121 caattg //