LOCUS       Exported                1055 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-puro-hTERT-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp 
            PM, Sedivy JM, Weinberg RA
  TITLE     Dissociation among in vitro telomerase activity, telomere 
            maintenance, and cellular immortalization.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.
  PUBMED    9843956
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(2..211)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7..142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(12..309)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(61..80)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     307..327
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(345..371)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(375..401)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             738..749
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
       61 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
      121 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
      181 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
      241 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
      301 ttccacaccc taactgacac acattccaca gggtcgactt atcctgcata gtccgggacg
      361 tcatagggat agcccgcata gtcaggaaca tcgtatgggt agtccaggat ggtcttgaag
      421 tctgagggca gtgccgggtt ggctgcggcc tccagggcag tcagcgtcgt ccccgggagc
      481 ttccgactca gctgcgtctg ggctgtcctg agtgacccca ggagtggcac gtaggtgaca
      541 cggtgtcgag tcagcttgag caggaatgct tggtggcaca gccactgcac ggcctcggag
      601 ggcagagggc cggcggcgcc cttggccccc agcgacatcc ctgcgttctt ggctttcagg
      661 atggagtagc agagggaggc cgtgtcagag atgacgcgca ggaaaaatgt ggggttcttc
      721 caaacttgct gatgaaatgg gagctgcagc acacatgcgt gaaacctgta cgcctgcagc
      781 aggaggatct tgtagatgtt ggtgcacacc gtctggaggc tgttcacctg caaatccaga
      841 aacaggctgt gacacttcag ccgcaagacc ccaaagagtt tgcgacgcat gttcctccca
      901 gccttgaagc cgcggttgaa ggtgagactg gctctgatgg aggtccgggc atagctggag
      961 tagtcgctct gcacctccag ggtccgggta tccagcagca ggccgcacca ggggaatagg
     1021 ccgtgggccg gcatctgaac aaaagccgtg ccacc
//
