LOCUS       Exported                 850 bp ds-DNA     linear   SYN 04-JUL-2021
DEFINITION  Human expression plasmid for SpCas9-VRQR variant: 
            CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG .
ACCESSION   .
VERSION     .
KEYWORDS    BPK2797
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 4, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat
       61 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt
      121 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct
      181 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa
      241 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag
      301 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg
      361 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta
      421 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca
      481 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc
      541 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata
      601 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg
      661 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct
      721 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga
      781 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc
      841 gattttctaa
//
