LOCUS Exported 2142 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion). ACCESSION . VERSION . KEYWORDS pBP-J23100 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2142) AUTHORS Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS TITLE EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. JOURNAL ACS Synth Biol. 2016 May 2. PUBMED 27096716 REFERENCE 2 (bases 1 to 2142) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2142 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 102..159 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(183..200) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(354..942) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(434..453) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1125..1219 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1240..1899) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1814..1833 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2061..2079 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2083..2126 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2129..6 /label=BioBrick prefix /note="BioBrick prefix for parts that start with ""ATG""" ORIGIN 1 ttctagaatc tagagacgca taggtctcac tatttgacgg ctagctcagt cctaggtaca 61 gtgctagcgt acagagaccc atgccgtctc attagctgca gtccggcaaa aaagggcaag 121 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct 181 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 241 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac 361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 781 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1021 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1081 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa 1141 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact 1201 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc 1261 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc 1321 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc 1381 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt 1441 gaaactcacc cagggattgg ctgacacgaa aaacatattc tcaataaacc ctttagggaa 1501 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg 1561 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac 1621 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg 1681 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 1741 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 1801 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 1861 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 1921 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 1981 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta 2041 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat 2101 ccttagcttt cgctaaggat gatttctgga attcgcggcc gc //