LOCUS Exported 2130 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion). ACCESSION . VERSION . KEYWORDS pBP-Tag_linker SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2130) AUTHORS Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS TITLE EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. JOURNAL ACS Synth Biol. 2016 May 2. PUBMED 27096716 REFERENCE 2 (bases 1 to 2130) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2130 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 83..140 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(164..181) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(335..923) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(415..434) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1106..1200 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1221..1880) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1795..1814 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2042..2060 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2064..2107 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2110..2129 /label=BioBrick prefix /note="BioBrick prefix for parts that start with ""ATG""" ORIGIN 1 atctagagac gcataggtct cagtacttta actttaagaa ggagatatat aaaagagacc 61 catgccgtct cattagctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt 121 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc 181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg 361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 1021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 1081 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga 1141 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc 1201 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat 1261 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc 1321 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg 1381 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg 1441 gctgacacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg 1501 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca 1561 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca 1621 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc 1681 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg 1741 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact 1801 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat 1861 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa 1921 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg 1981 atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 2041 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga 2101 tgatttctgg aattcgcggc cgcttctaga //