LOCUS Exported 2136 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion). ACCESSION . VERSION . KEYWORDS pBP-Strep(II) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2136) AUTHORS Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS TITLE EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. JOURNAL ACS Synth Biol. 2016 May 2. PUBMED 27096716 REFERENCE 2 (bases 1 to 2136) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2136 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 29..52 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" terminator 89..146 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(170..187) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(341..929) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(421..440) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1112..1206 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1227..1886) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1801..1820 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2048..2066 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2070..2113 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2116..2135 /label=BioBrick prefix /note="BioBrick prefix for parts that start with ""ATG""" ORIGIN 1 atctagagac gcataggtct cataaatgtg gagccacccg cagttcgaaa aaccgcataa 61 gagacccatg ccgtctcatt agctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct 121 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg 181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc 361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 1021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 1081 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc 1141 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca 1201 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt 1261 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga 1321 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg 1381 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag 1441 ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt 1501 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg 1561 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg 1621 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga 1681 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc 1741 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga 1801 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg 1861 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac 1921 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg 1981 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 2041 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc 2101 taaggatgat ttctggaatt cgcggccgct tctaga //