LOCUS Exported 2036 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Secondary module (BpiI) site in pTU2a backbone. ACCESSION . VERSION . KEYWORDS pTU2_2a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2036) AUTHORS Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS TITLE EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. JOURNAL ACS Synth Biol. 2016 May 2. PUBMED 27096716 REFERENCE 2 (bases 1 to 2036) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2036 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 2..59 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(83..100) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(254..842) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(334..353) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1025..1119 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1140..1799) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1714..1733 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" misc_feature 1964..1985 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat 61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct 301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc 1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat 1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat 1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgacacgaa aaacatattc 1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga 1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt 1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga 1921 cgtcctataa gtcttccata tgcacgcatg cgaagacaag tacgaattcg cggccgcttc 1981 tagaggtctc actatatcta gagacgcacc gtctcaccgg gtacagagac cctgca //