LOCUS Exported 8440 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral expression of human VEGFC. ACCESSION . VERSION . KEYWORDS pQCXIP-VEGFC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8440) TITLE Grusch plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 8440) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8440 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 309..512 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 513..688 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 751..950 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1151..1567 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1497..1519 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1535..1551 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" enhancer 1604..1907 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1908..2111 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2108..2132 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 3567..4140 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3734..3751) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3961..3980 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4173..4772 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4173..4192) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4669..4689 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 5146..5571 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" primer_bind complement(5674..5696) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5832..5852) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5837..6166 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6017..6152 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6079..6098 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 6188..6207 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(6288..6305) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6459..7047) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6539..6558) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7218..8078) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7841..7860 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8079..8183) /gene="bla" /label=AmpR promoter primer_bind 8251..8269 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 8369..308 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga 2281 tccatgcact tgctgggctt cttctctgtg gcgtgttctc tgctcgccgc tgcgctgctc 2341 ccgggtcctc gcgaggcgcc cgccgccgcc gccgccttcg agtccggact cgacctctcg 2401 gacgcggagc ccgacgcggg cgaggccacg gcttatgcaa gcaaagatct ggaggagcag 2461 ttacggtctg tgtccagtgt agatgaactc atgactgtac tctacccaga atattggaaa 2521 atgtacaagt gtcagctaag gaaaggaggc tggcaacata acagagaaca ggccaacctc 2581 aactcaagga cagaagagac tataaaattt gctgcagcac attataatac agagatcttg 2641 aaaagtattg ataatgagtg gagaaagact caatgcatgc cacgggaggt gtgtatagat 2701 gtggggaagg agtttggagt cgcgacaaac accttcttta aacctccatg tgtgtccgtc 2761 tacagatgtg ggggttgctg caatagtgag gggctgcagt gcatgaacac cagcacgagc 2821 tacctcagca agacgttatt tgaaattaca gtgcctctct ctcaaggccc caaaccagta 2881 acaatcagtt ttgccaatca cacttcctgc cgatgcatgt ctaaactgga tgtttacaga 2941 caagttcatt ccattattag acgttccctg ccagcaacac taccacagtg tcaggcagcg 3001 aacaagacct gccccaccaa ttacatgtgg aataatcaca tctgcagatg cctggctcag 3061 gaagatttta tgttttcctc ggatgctgga gatgactcaa cagatggatt ccatgacatc 3121 tgtggaccaa acaaggagct ggatgaagag acctgtcagt gtgtctgcag agcggggctt 3181 cggcctgcca gctgtggacc ccacaaagaa ctagacagaa actcatgcca gtgtgtctgt 3241 aaaaacaaac tcttccccag ccaatgtggg gccaaccgag aatttgatga aaacacatgc 3301 cagtgtgtat gtaaaagaac ctgccccaga aatcaacccc taaatcctgg aaaatgtgcc 3361 tgtgaatgta cagaaagtcc acagaaatgc ttgttaaaag gaaagaagtt ccaccaccaa 3421 acatgcagct gttacagacg gccatgtacg aaccgccaga aggcttgtga gccaggattt 3481 tcatatagtg aagaagtgtg tcgttgtgtc ccttcatatt ggaaaagacc acaaatgagc 3541 taagaattcc gccccccccc cccccccccc tctccctccc ccccccctaa cgttactggc 3601 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 3661 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 3721 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 3781 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 3841 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 3901 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 3961 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 4021 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 4081 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 4141 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc 4201 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 4261 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 4321 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 4381 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 4441 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 4501 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 4561 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 4621 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 4681 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 4741 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga 4801 ccgaaaggag cgcacgaccc catggctccg accgaagcca cccggggcgg ccccgccgac 4861 cccgcacccg cccccgaggc ccaccgactc tagtcgaggg ctgcagcgct gcagaggccg 4921 agtgcagaac tgctccaaag ggacctcaag gctttccgag ggacactagg ctgactccat 4981 cgagccagtg tagagataag cttatcgatt agtccaattt gttaaagaca ggatatcagt 5041 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc 5101 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac 5161 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata 5221 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt cgaccctaga 5281 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 5341 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 5401 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 5461 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 5521 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 5581 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 5641 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5701 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5761 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 5821 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 5881 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 5941 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 6001 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 6061 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 6121 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt 6181 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 6241 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 6301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 6661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7501 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 7561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 7621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 7681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7801 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 7861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 8221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 8281 caagaattag cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt 8341 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa 8401 tcaattacgg ggtcattagt tcatagccca tatatggagt //