LOCUS       Exported                 307 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-PuroR for targeted DNA methylation in mammalian cells; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    pdCas9-DNMT3A-PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tgccgccagt agttcttcat cttcttcacg acctcttcgg agggcacgtt gtcgctcttg
       61 ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga tggagtcgtc cttcagaaag
      121 ctctgaggca cgatagcgtc cacatcgtag tcggacagcc ggttgatgtc cagttcctgg
      181 tccacgtaca tatcccgccc attctgcagg tagtacaggt acagcttctc gttctgcagc
      241 tgggtgtttt ccacggggtg ttctttcagg atctggctgc ccagctcttt gatgccctct
      301 tcgatcc
//
