LOCUS Exported 9119 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral transfer vector for tet-inducible expression of EGFP. ACCESSION . VERSION . KEYWORDS tetO-FUW-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9119) AUTHORS Kong YP, Carrion B, Singh RK, Putnam AJ TITLE Matrix identity and tractional forces influence indirect cardiac reprogramming. JOURNAL Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474. PUBMED 24326998 REFERENCE 2 (bases 1 to 9119) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9119 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..181 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 228..353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 846..1079 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1264..1308 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1606..1723 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 1780..2050 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2083..2121 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2118..2142 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2227..2946 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2227..2943 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2272..2293) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2533..2552) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2880..2901 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2977..3565 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3030..3050) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3448..3459) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(3568..3584) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3569..3585) /label=pBluescriptKS /note="For pBluescript vector" LTR 4090..4270 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(4296..4313) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4302..4526 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4572..5000 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4659..4678) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4869..4890 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5009..5029) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5014..5343 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5194..5329 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5256..5275 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 5391..5438 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 5457..5831 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 5961..6082 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5998..6017) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6052..6071 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6131..6147) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6131..6147) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6144..6166) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6155..6171 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6179..6209) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6224..6245 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6362..6379) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6533..7121) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6613..6632) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7292..8152) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7915..7934 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8153..8257) /gene="bla" /label=AmpR promoter primer_bind complement(8332..8351) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 8523..8902 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8904..9102 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9057..9077 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" ORIGIN 1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 1741 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa 1801 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 1861 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 1921 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 1981 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca 2041 gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg 2101 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 2161 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc 2221 tctagaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2401 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 2461 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2641 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 2701 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2821 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 2941 aagtaaggat ccgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt 3001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 3061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 3121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 3181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 3241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 3301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 3361 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 3421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 3481 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 3541 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat 3601 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 3661 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 3721 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 3781 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 3841 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga 3901 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag 3961 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta 4021 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg 4081 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 4141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 4201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 4261 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 4321 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 4381 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 4441 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 4501 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 4561 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 4621 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 4681 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 4741 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 4801 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4861 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 4921 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4981 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 5041 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 5101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 5161 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 5221 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 5281 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 5341 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt 5401 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg 5461 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt 5521 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg 5581 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca 5641 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg 5701 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt 5761 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg 5821 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg 5881 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 5941 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 6001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6061 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 6121 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 6181 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 6241 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 6301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 6361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 6421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 6481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 6541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 7021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 7081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 7201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 7441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 7501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 7561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 7621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 7681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 7741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 7801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 7921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 7981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 8041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 8101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 8161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 8221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 8281 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg 8341 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga 8401 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa 8461 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg 8521 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 8581 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 8641 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 8701 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 8761 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 8821 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 8881 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 8941 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 9001 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 9061 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtact //