LOCUS Exported 913 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pMKO.1 puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 913) AUTHORS Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD TITLE Lentivirus-delivered stable gene silencing by RNAi in primary cells. JOURNAL RNA 2003 Apr;9(4):493-501. PUBMED 12649500 REFERENCE 2 (bases 1 to 913) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..913 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 62..302 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 62..82 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 233..252 /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(366..386) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 371..700 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 551..686 /label=SV40 ori /note="SV40 origin of replication" primer_bind 613..632 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(710..729) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" ORIGIN 1 cagccctcac tccttctcta ggcgccggaa ttgaagatct gtcgacaagg tcgggcagga 61 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga 121 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag 181 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca 241 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg 301 acgaaacacc ggtacgcgta ctagtactcc catatggaat tcgccagcac agtggtcgac 361 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggcttcccag caggcagaag 421 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 541 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 601 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 661 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tgaccgagta 721 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 781 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 841 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 901 gtgggtcgcg gac //