LOCUS Exported 767 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP. ACCESSION . VERSION . KEYWORDS PX458_HHEX_2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 767) TITLE Mendenhall CRISPR tagging plasmids REFERENCE 2 (bases 1 to 767) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..767 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 27..50 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 57..77 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 acggagacta caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa 61 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg 121 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc 181 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga 241 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg 301 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca 361 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg 421 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct 481 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca 541 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact 601 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc 661 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg 721 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaa //