LOCUS Exported 20259 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested. ACCESSION . VERSION . KEYWORDS pEMS1153 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 20259) AUTHORS Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM TITLE A regulatory toolbox of MiniPromoters to drive selective expression in the brain. JOURNAL Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PUBMED 20807748 REFERENCE 2 (bases 1 to 20259) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..20259 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" regulatory 3993..4002 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3999..4715 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3999..4715 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4044..4065) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4305..4324) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4652..4673 /label=EGFP-C /note="EGFP, forward primer" CDS 4728..4745 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" CDS 4758..4778 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4782..4802 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4806..4826 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 4833..4866 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 4989..5070 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5026..5045) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12715..12736 /label=F1ori-F /note="F1 origin, forward primer" promoter 12899..13003 /gene="bla" /label=AmpR promoter CDS 13004..13864 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(18071..18093) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 18179..18319 /label=bom /note="basis of mobility region from pBR322" primer_bind 18234..18253 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(18334..18351) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(18505..19093) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(18585..18604) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(19264..20124) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(20125..20229) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccca caggttttac tgctaaatag gaggtcaatg tacaatgaac 181 atgatttatt gggtcccttc caattttaag agtctctgca gactggcatc accattctct 241 ttccctggct attaaaaatg caggtggaaa aattgcttca ttcaactgta aggctattca 301 cctctctgcc caagatagga aggcagaaat gactttattt tgagcatttc attcccaact 361 ccctaattga accaccttcc tttgaattct aaaatggtat catttccatt ttataaggtt 421 atcaagggca tgtatttctt caaaaatggc accatacaga gtagcttcct atgtgatctg 481 atttgaggag ggccaaggga gattcaattg caccaaatta tttttttccc attggtcctg 541 ctatacatct taaaatccct ctattctcta ttgttgtaat ttttttatat atagctatca 601 aaatgtgaat gttatctagg ttttatctat ctttaacatc atagccagac ttagtctggc 661 aaagcaatca ccgtaaagtg tgagcatggt ggagaagcca catcgccatt taggtacagc 721 tgggtcagcc atgagcagct ctagtcggca ctgcatcgct aagtggactc cgggtgcctt 781 ttcttaattg aatgaaatct cagatttcaa tttatttcca ggctgcataa tgtgataatt 841 agaagcttag ttacatctaa tgccctgaat ggaggtccta ggattgtctc acaacagtca 901 agaggtctgg atggataatt gccaccactt cacagtaaga attgccaacc tcagagctct 961 aaggagagaa tgcccatgga actgagggct ttgaaaatcc actcaagtgt tgccccaatg 1021 ttagcattgt tatcaatgaa gagattttgt cttttatggt gctggattgt caggaagggg 1081 tttcttttat atggcacgga tttccttttc attcatcagt ccacagtgag gtggttttaa 1141 tgaggcacaa gctgggctga gatcaagatg aaagcagaaa gatcaagggc caacgaggaa 1201 gaaaaaagaa tactcagccc aggagcagct tccattcttc aaggacaatc ttgaagaaga 1261 tttcctaggg gaagcatttg cagttttaat aacagacttg aaattgaagc tcaatctatt 1321 tctgggcaat aaaggaagta tctttagtgt tatctgattt atcttagaat tagatagatt 1381 caaaatgaat agacagcaat ccctggaaca tttcacaggg tgtaactcaa gccaagtaat 1441 tccagaaaag caagtagtac tctatatgct caagcctcaa aatcaagttc agaaatccaa 1501 caggctcgtt tttgaactct tgaatagaaa ctggaaagca aggatactgt atttgggtta 1561 cacatactac tttatagcat acactatttg acatcactcc ttattaaaaa tattccttca 1621 tctctaaagc ttaaagtgaa tggtgttatc tccaatagac actggaaaaa aagttggaga 1681 acaaaacccc caaatctgct cactatctta ctttatagaa aatgttttga aatattgata 1741 cctctcttta gatggttata cataagagat ttggttgtta ctgaagcatt tcatttatgc 1801 tgagacaagt aatacaaata tgtatgaagg tgacttgcaa gttgagacat tatgtcatgg 1861 gggctcaagt agggaccaag tgccatgagg tgtcatattt ccagactggt ctgaggtcaa 1921 gatgctaagt gagttcatct agctgactgc ccaggcctcc acatctcatg gtggtattcg 1981 tgaagtaaaa gaaaactgat ttttctgtgt ttaaaaaaaa aacacacaaa agatttgaaa 2041 ggaaaggtga ccaagtattg ttgacagaag taaacagaaa gttgagatca aagacagttt 2101 tagttcttaa ctagaaaacc aaagtctggg gtaaatgaaa caatagaatg taaaattcag 2161 cagtgacctg gtcctatgaa tacaagcaat tcaccatata ctctccaaag aaagaaataa 2221 agaaaaaaca ctttgtaaaa cagtttcagc tggtgtctca gctagtatga aaattgctca 2281 tcaaatgaga gataaaattt tagtggaaat tgaaaagaaa atttgtgtta taatgaaata 2341 cacaagaagc ctatgcctgc acagaaaact gattcgggtg aaacccagag atttccataa 2401 ctgagatcgt gaatatttgt gaaaaactag atacttgtct ttcaaatgga ttaagtatag 2461 gaagaaactc acatctctct ctcaatcatc tgtatatcta cctatcacat atgtatatat 2521 gtttctttca tctatccatc catctccatt ttatctgatt gacttttatg ttgaaagggc 2581 aaatatcatt gagagcttta gcgcacatca cagggggaac taggagaatt tggaactctt 2641 tccacctgtg ccgtggttgt ctccctacct ctatgtccat tggctgcaat aaagataatg 2701 gaggttaaaa aaagatcctc aaaggaagat actgtatgtt ctactggaaa tatgtttccc 2761 ttattctgaa atcagtaggt aatggattgc tgcctggtga acagaagcta gaaagctgtt 2821 tggcttttag gggttccagt ctcctcagtt cctatagatg aggagcaggc actggaggtg 2881 atggagggtg tgaggaaacc atggactggg gccaggtcac ttcttcccta actgaacgtc 2941 ctgaagtgct tttcacggtc tctttttaaa acagtaataa aatatttcag aaataaaaat 3001 caaagtcctt atcttagctc acaaaatcat tttcgccgca ccccttacta accccggttt 3061 gtgccccttc actatgggag tagctcttgt catcatctaa aaaacttgaa ctgcattttg 3121 agagatttat tggccttgct tctgcaggct cataactcct ttatgtgctt gctcttggga 3181 ggaaaaagca gatagacgtt taaaagctgg atgttcctgc acacccctct cctgatgctt 3241 cattcttttc caggcagaaa gatggtggga ctctgtctct agcaaaggga tatttccaga 3301 tactgaggtg ttgtcagaga cacctggtca gggaggttag gagaaggggc atccaggtcc 3361 accccctcca actggctgct gctttcctgg cagggctgca ctgggacacc tcacttcctt 3421 cccacttccc cttcctcctc ccattcgctg tctctttgca cacccctaat atggcctttc 3481 atagtaagag gtcaatatgt tttcacactt gggaaatctc attcaagaat ttttgtcaat 3541 ggacaagtca taagaagccc ttccatttta gggctcgttg acgtcaccaa gaggcgataa 3601 atatctgttg atataattgg atgtgagatt cagtgttgag atagcaaaat tctgcccctc 3661 gttccttggc agggccctat gatttatgca ggagcagagg cagcacgcaa tcgagctgtc 3721 aagagagcgt cagcttatta ggcaaatgct gcgtggtttt tgaagagggt cgacactata 3781 aaatcccact ccaggctctg gagtggagaa actcagagac caagtccatt gagagactga 3841 ggggaaagag aggagagaaa gaaaaagaga gtgggaacag taaagagaaa ggaagacaac 3901 ctccagagaa agcccccgga gacgtctctc tgcagagagg cggcagcacg gcgcgccgaa 3961 gttcctattc ttcaaaaggt ataggaactt ccgccaccat ggtgagcaag ggcgaggagc 4021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 4081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 4141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 4201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 4261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 4321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 4381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 4441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 4501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 4561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 4621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 4681 ccgggatcac tctcggcatg gacgagctgt acaagttatt tctgaactgc tgcccgggct 4741 gctgcatgga accggatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg 4801 tagatccaaa aaagaagaga aaggtaatat aagaagttcc tattctctag aaagtatagg 4861 aacttcagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac 4921 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 4981 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5041 atttcacaaa taaagcattt ttttcactgc gaattctcgt aaaactcttc atggcagtag 5101 ttattattct ctctctctct ctttttcttt tttcttgaga caggatattt ctctgttgcc 5161 caggctggag tgcagtggca cagtcttggc tcactgcagc ctggacctcc tgggctcaag 5221 ccatcctccc acctcagcct cccaagtagc tggggctaca ggcacatggc caccaggccc 5281 agataatttt tcatttttgt agagactgag tctcaccatg ttacccaggt ttattattct 5341 cattttttag atgaagagac tgaggtccag agaagctcaa tgacttgcct agttttacaa 5401 atctcctgcc atcacatacc cctcagcgtc cttaataaga gggaggccac caactatgtg 5461 ctgggcactg tggtggatgc tggagctata gggttgagta tataagaaat ggtgttgctg 5521 gagcaactgt tgcttgctta cctgacctat ctgagaatta attagcaggg gaacatattt 5581 ttgttttcag attcaatata agaacttgtg tgggcaaaaa taaagatcag tagtaataac 5641 agtagttccc atttgctgac tgtactgtcc taagtgcata tatatataca tacacacacg 5701 catacctata ctcctctaat actcaaaatg atcctgttta tgtattgtta atatgctcat 5761 tttattttta aatttttatt tatttttatt tttatttatt tttgagacgg agtctcattc 5821 tgtcgcggag gctgaagtgc agtggtgcga tctcagctca gtgcgacctc cgcctcccgg 5881 gttcaagtga ttctcctgcc tcagcctccg gactagctgg gattacaggc gcccgcctcc 5941 acgcccagct aatttttgta tttttagtag agatggggtt tcgccatgtt ggccaggctg 6001 gtctcgtact cctgaccttg agtgatccac ctgcctcggc ctcccaaagt gctgggatta 6061 caggcatgag ccaccgcgcc gggctaatat gctcatttta gtgaggcaaa aatagaggct 6121 cagagtctga tttgtacaaa actacagagc agttaagtgt cctctcagat gtgtaccctg 6181 atctgggtga ctctaggact ctaggtctca actgttacaa ccagttaagg gtttggggaa 6241 gcactgggcc aagagtcagg aaaatggaag ccacaggtag tgcaaggtct tgggaatggg 6301 acgtctggtc caaggattca cgcgatgact ggaacccgaa gagccggggc ccggtttacg 6361 gccgccatga agcaacgcgc gccggtaggt ttgggaatca gggagccctc tgaataggag 6421 actgagttgg gagggaaagg ggcttcgctg ggggagcctc ggcttcttct gggagaaaat 6481 tcccacggct acctagtgag cctgcaaact ggtaggcgcc ggcgtaggcg cgcgggcggg 6541 gccgggggcg gggcctgcgg ggcgtggcgg ggcgggcaga gggcggggcc tgcttctcct 6601 cagcttcagg cggctgcgac gagccctcag gcgaacctct cggctttccc gcgcggcgcc 6661 gcctcttgct gcgcctccgc ctcctcctct gctccgccac cggcttcctc ctcctgagca 6721 gtcagcccgc gcgccggccg gctccgttat ggcgacccgc agccctggcg tcgtggtgag 6781 cagctcggcc tgccggccct ggccggttca ggcccacgcg gcaggtggcg gccgggccct 6841 gaggcgcggg atcgatccat gacacccccc cgccccccat atctcactat aattcactgt 6901 caaatggctt ttatgatttg gagtttgggg atacttttaa agacagggtc ttggtagctc 6961 ctagatggct tgggattcac ttagtaaccc ttgctggcct aaaattcagg aacttctacc 7021 tcatcttccc aagtactggg ttagtaggga tgtccagcat atttagcttg ctttaatgat 7081 tttgctgtac ttttttctgt gtgtacaggt gcatgtatat ttataaaact aacataatat 7141 attttacccc caaagtacca taaccctaat taatgctatt aaatgttttg tatgctgggc 7201 tctgggcata ctaatataac caatattata caatgcaaaa caattttgga atatcttctg 7261 taaaattatc ttattagtaa gttgtaacca tctcagactt ctaagtttga gtttgtaaag 7321 gatcagtttt ttttggtgac ctacacacac acacacacac acacacacac acacacacac 7381 aggggggtgg attctgagaa tttagagaat ttagatggtt aataccagtt tttattttta 7441 ttctgtgtga tatttattta ttaagattta tttatttatt acatgtaggt acactgtatc 7501 tgtcttcaga cacaccagaa gagggcatca gatctcgtta cagatggttg tgagtcacca 7561 tgtggttgct gggatttgaa ctcaggacct ctggaagaac agtcagtgct cttaaccgtt 7621 gagccatctc accagccccg tgatatttta tttaacttac tagaatatac tgtgttaaaa 7681 catattttag gggattgggg agatggccca ttctgtaaag tgtctgccat gtcatcttca 7741 ggacctgcgt ttgtaacccc agcatggaga agacagagac agggagaccc ctgggcttcc 7801 tggcaagcta gactagcaaa tttggttaac taggttcaat gagagactca gtctcaaaac 7861 ataagaagtg aattttttag acccaatttc aatctctgaa ctccatacgt atgcacatgt 7921 acctgcatat aaatatatat acacacccat aaacacatgc acacacaaaa aatggttcaa 7981 aagttcttaa aatgtataag agtagaaagt gtagagccgg gagtggtggc gcacgccttt 8041 aatcccagca cttgggagca gaggcaggtg gatttctgag ttcgaggcca gcctggtcta 8101 cagagtgagt tccaggacag ccagagctat acagagaaat cctgcctcaa aaaaccaaaa 8161 ataaagtgta tatactaatc tttcatgcct aaaatattta tttttgccac atttttcata 8221 cctctttaat atttttcttt ttctctttgt gcttcaaagg aaattttgtg tcatgtttta 8281 cttttcattc ttgactctat tcagcagtaa gacgcagcat ggctttctta gagaagtata 8341 atctattaca aaacggtaac atttaactgc tctacatacc ctcttgtagc taaattcctc 8401 tgttaaacta agacttatta tctaaacgta attaaatgtt gatatcttat aatgctccac 8461 tttgaaacag ctgtctttct tttgcagatt agcgatgatg aaccaggtta tgacctagat 8521 ttgttttgta tacctaatca ttatgccgag gatttggaaa aagtgtttat tcctcatgga 8581 ctgattatgg acaggttagt aagacctcga ttgaagttat ttttagaggg tgtgtgtgtg 8641 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt aagagagaga ggagaggcat 8701 gtgtgatata tatgtgaagt gtattgcatg tacatggaat ccagagaagg acattaggtg 8761 tcctactctg ttgctctctt attcccttga atcagtgtct cttgctgaac ctggggcata 8821 catactgatt tggctaggct gcctggctag caagacccag ggatctgctt gtctctgccc 8881 cccagtgctg acgttacaag caggtgcaac cacatctggt ttttatatgg gtactggggg 8941 atctgaactt agggcctgat gcgtgcccaa gtgctctcac ccgccaagcc acctctccag 9001 ccaaagggtt ttttttcttg tattaattct aacttagcaa ctgttgagta gttgatgttt 9061 gaatataagc taagccaact tattagaata aatgttttct attttttact tgtatgtgtg 9121 agtgcctgca tgtatgtatg tgaagcatat gtattccagg atgtcagaat tgtgatggat 9181 tccctggaac tggagttaca gaccattgtg aaccactgtg tatgggtgct gggaactgaa 9241 ctttaggtcc tctgcaaaaa cagtaaggat cttaactgat aaactatctt tctagcccta 9301 aggctttcta cttttaaagt tcgtttctct ttctctctct ctctctctct ctctctctct 9361 ctctccctct ccctccctcc tcctcctcct cctctttccc ccctctcgcc tcctctttct 9421 ctctcccctc cctctctctc tctcacacac acacacagac agagagagga gagagaaagg 9481 aagccttact aattaattac ctagttttag acataactag tgggaatcca tagtaagtaa 9541 atcaacattc ttgaagagag gttagaagaa ttggctctcg ttgtccatcg aggctgtata 9601 agattattct tctgttttct cagcttacca tacatatttt ctacgtagac aataccaatt 9661 caaatacact gtacgtaaag tatatgcgca cacacacaca cacacacaca cacacacaca 9721 cacaagttac atattgtcat atagtactaa aatttatgga ctttttcata ttaatacatc 9781 tagagctatc atgtttttta tcttttaaaa attactttac ggggctacag agatgtctca 9841 gcagttagga gcactgactg ctcttccaga ggtcctgagt tcaattccca gcaaccacat 9901 ggtggctcac aaccatctgt aatgggatct gatccactct tatggtgtgt ctgaagacag 9961 tgacagtgta ctcatataca tgaaataaat ctttaaaaaa ttactttacc atcagggtat 10021 atatatatat atatatgtgt gtgtgtgtgt gtgtgtgtat gaagtatttg ctatttgaga 10081 gggtctcact gtttagctca gtctggcttg gaacgttctg tatagcccag actggcctca 10141 actcagcagt ccccctgaaa ggctctcata tgtggagatt acaggtatgt gctactgtgc 10201 ccagccctac atgttacttt gtttttgaaa tagggtatca aatagcttag actggcttct 10261 aagtcagtgt gtagctaagg atggtctgag cttctgaacc tgctacttca actcctggtg 10321 tgccatcatg gccagtttgc acagttgtga gagtagaact caggacctcc tatgtgttac 10381 tagacatgtt ctctaccagc tgagctacat cccaattcgc ctacatttac ttttattttt 10441 taaaagattt atttatgtat acagcactct gcctgcatgt acatctgcat gccagaagag 10501 ggcaccagat ctcatcatac atagttgtga gccaccatgt ggttgctggg gattgaactc 10561 agggcctctg gaagagcagt cagtgctctt aacctctgag ccatctctgt agccccctac 10621 atttatttta aagattatag gctattctat tcatagatgt gccataactg atttaaccag 10681 atcttcatta ttttgtactt agtagagtat attagaggga atgaatctta aatacacatt 10741 ttgacacttg ctcagcttta gacaagtaaa aatcactggt caaggaaatg gtgcttagtg 10801 gatgcttctg aactgtcaaa ttgtcctcca acaagttaca tcattttata cttccaacgt 10861 tatgtgagag ttcatttttc tttaggctct tctagtattt tatatttgag aaataaaatt 10921 taaccaaaat ttgccaatct agtaagcttg tttttatttg tatctttaaa ttttatgata 10981 gatgttatag tgtactctcc tctcccctcc cctcccctct cctcctcagc aggatcttgt 11041 tatgtagccc taagggatct aatacttact ctgtagtcca ggctgtcctt cagctcatga 11101 gacacaccat catgcttggc atgtttcttg ttctgaaagg tatctggttt taattttggt 11161 tgagctggtg tgttggtgta tatctgtaat ctcagcacag gcaagtagag acaaggatta 11221 tgattacagt ccagcctggg ctatttagag ggattctctt taatctttcc tcatgcccca 11281 aaatcttacc tttggtatat gaaaaatagt ctccacttct gcaaaatatt gctttatgaa 11341 gtaagaatta attcccttca tagagacaag gaatgtgtcc tgtaaaagtt taatgtgtaa 11401 gaagtatttg ttataaaaga taaatattca gaatcttctt tttaattcct gattttattt 11461 ctataggact gaaagacttg ctcgagatgt catgaaggag atgggaggcc atcacattgt 11521 ggccctctgt gtgctcaagg ggggctataa gttctttgct gacctgctgg attacattaa 11581 agcactgaat agaaatagtg atagatccat tcctatgact gtagatttta tcagactgaa 11641 gagctactgt gtaagtataa ttaacttata attaaaaaaa tagggccatt ctagttttat 11701 ctatattttt tttaaacttg tgcaaactat gctacataat caacttactt gattatgtaa 11761 tatatatagt aatagttgga attactatta ttacatattt atttttctgt gctggggtag 11821 aacccaggga actaagtact gtaataaccg agcttcattc atcctagtca tttttaattt 11881 tttttttttt ttttgttttt tcgagacaag gtttctctgt gtagccctgg ctgtcctgga 11941 actcactctg tagaccaggc tggcctcgaa ctcagaaatc tgcctgcctc tgcctcccag 12001 tgctgagatt aatgacgtgc accaccactg cctggcattt ttttaaagtt ttaatttgag 12061 accctttctc actaacttgc tcacactatc ctcgaactca ttacgtgggc tggtctagaa 12121 atttcagtca acttgcctca gcttctcaag tagctgggat tacaggcagg tgctaccact 12181 tctggccatg atcattgttg ttgttgctat tattattatt attatttcac tgatacttca 12241 tatcacaaca cagaatagta actgtagttc atgaccaaca aaaaagttgt acagtttctc 12301 tgttgtctta ttttgatctg gattttgatt tgtttcatgg cactgggcag gctaatctca 12361 aactgcatgg ctcaaatagt ccttctgtct taatctccca tgttctggaa gtataggtat 12421 cactacacct agctgtattt tcatatttta aaaaaaacgc ctaagaattg ctattgaatg 12481 aggcggagag atggctcagc agtcacaagt acttgtagag gactggggtt tggttactag 12541 cacctcaaag atggcttaca attatctgta gctccaattc cagaggatct gtgtggtaaa 12601 gctgcatgta tatgggcatg caggtaaaac tgctaaacat ataaaaatag ctcagtctta 12661 agagaattgg tcgacttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 12721 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 12781 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 12841 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 12901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 12961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 13021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 13081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 13141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 13201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 13261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 13321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 13381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 13441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 13501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 13561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 13621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 13681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 13741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 13801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 13861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 13921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 13981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 14041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccaggtcg 14101 agtagggata acagggtaat ggatcctcgt tgggcacctg cactcatgta cgcaggccca 14161 catatagatg tacacacgca tatcattcaa aatagaaatt atttatacaa gtagtgcgtt 14221 ggagagctgg ctcaggagtt gttaatgctt gctacccttg caaaggaccc aagttcaatt 14281 tccagcacct gtgttgggca tctcacaacc acatgtaaat ctagtttcag gggatttcac 14341 ccctcttctg aactcttcag gcacttatac ccacaaatgg acatctccac atagagacac 14401 atacttttaa aaaagtaatc tagggtctag agaaatggtt atagaccacg gactgggatc 14461 agttcccaga agccacatcc tgtggctcac aattgcctgt gaatcaagtt ctagatctgg 14521 tggcctcgtc tagcctgcag tcatcaacaa acacatgcac acatccatac acacataatt 14581 taaaagaaag cgaaagtaac ctataatgct aaacaaagca cattctcagt gtagactgca 14641 gtggtgatga accttgaaaa tatattaata gaagccagac acgtaagtca caaatggtat 14701 aactcaattt atatgaaatt cccagagagt ccaaagttaa aaaaaacaaa aaggcagatt 14761 attggttgtc agtagctaca gagatgggac attgaagata ttaataggta tagggttgag 14821 gattcatggc ctttccctct tccatgttag catgtctatt gttgttaacc ttattcaact 14881 catgttaggg caatcatatt gatgagactt aatgagtata gcttgacatt cctagaagac 14941 agagtctcac atcagactct ctgatcctct gactcttaga atctttcaac accctcttct 15001 gaaatgatct ctgagccttt ggttgtgact gaattatgag aaagaatttt atgttgaatg 15061 aattttactt cagcattttt acaaaactga agctggggat ggcgtttcat gcgtgcagtc 15121 cctaccattt gcctataagc ctggaggagg cagaagtaga attaggcttt tcagggacat 15181 cttcagctgc atcatgagct tgaggacaac ttgagctaca taagattccc atccccaccc 15241 ccacacacac acacacatct agtatgtacc tagtatgcag tcttttcata tgtttaaaat 15301 gtagaaaggt atatgtgaag tctccctttt ccccacaact tagtccctcc aaagtattgc 15361 atcagtttcc ttgtacatga ttttatgcat atataagata cactatttct ctctctctct 15421 ctctccttcc ctccttccct ccaacctctg ggtatgagtt gagggttatg tatgtttcaa 15481 gttgtaataa gaattgccac tcaaacattt catcccattt taagttaagt ttgtctgatt 15541 ttccacttta gaagggcagc catgatggct catgcctata atcccatcct agcacttgta 15601 agcctaatac ataagactgg aggattgtca tcagcttgag gctagcctgg tctacctaat 15661 gagttccagc cagccaagag tagaatataa gaccctgtct ccaaaaaatg taccaaaggg 15721 gctggagggg taactcagtg gttaagaaca gtagctgctc ttccagagga cctgggttca 15781 attcccagca cccacatggc agcttgcaac tatctgtaac tgtccagctt tgggatatcc 15841 gacaccatta taccaattac ataaaatgaa aaaattgtaa aatgtgctaa aatgagtggg 15901 gagggggagc atggtgtggt tctgtgggct tgggattttg ccatgaatcc atgactatat 15961 ctcagcaata tgtggattat ttctggaaac ctgtggggca tgggtgtatg ggaagagggg 16021 agagagagag agcccgcatc cagccagagt tcctgtgctc tgggcaggcg gacgtgggag 16081 gactgctgga cgatttccac ttggccctgg gtgggcatct ggctgtgtga agccactgac 16141 cccactcagc ggggggtaga taaggggcat ttcccatacc aggttctcag actccagcat 16201 tccacgctgg atacagcaga agagctgaga acagaatagg ggccccaggg agacactctc 16261 gtcccagaga taaagaaggc agagggagag agattcctac gcaggcgaga ggccttagtc 16321 tgggccttga tgaacagaga cggtctatgg ttttagagct ttattgtaga aaggcagggg 16381 gaaagagaga aggtagaaag agagagacca gccatggcca agaggagaga aggggggaaa 16441 ggagagagag aagaaaggct agagagtaag aaaggttaaa gagacagaag taaaagctta 16501 gagtaagaga gagagagaga gcaagaggga gagtaagaaa agtaaaagct taagagagcg 16561 aggaggggcc agcccctcta atagtgggct tgttatcttg ttgttgctag gtaactgtga 16621 agcgtctagc ctgaaggcca gaagcttggg acattgtcta tgtgactgaa agccatgagc 16681 ctctactgtg ggggctgtgg aggtggtaac ttggacagga gccaggggtt ccaggagaca 16741 tgagggaatg ccttctgtcc catgtaggta ggaattacca ccaccggctt tcaccgtagt 16801 tcactggaga ctgtttgtac atagcccatt gccccacaga aaccaggcac tcactgtttc 16861 atttgcataa gaattagaag gctttatttt ggctgggatt ggtgacttgc cccagaaatt 16921 ccagcttttg ggaagccgaa tcctggagaa ccaggagttc aaggtcatcc tcaggtacct 16981 catttgttca agaccagcct gtgctaccat gagaccttgt ccctactccc ctcaacccca 17041 aaacacgctg ttttcatttt ataggttgta ctaaccatgg aggatggtca tttgcctttc 17101 tcttgatttc agaagcgtag cgcgggcttt gcaccatgat ctagtgtttc cagataggct 17161 tgcctctcca cagccaggct tatcctgctt cagtcccatg tttggcaagg gcaacatatg 17221 actgaagaac ttgcatagcc ttatggtgta tcatgactct tagccccagt ttataactgt 17281 ctaacagctg gtggcttttg tgaaaaagga aagcatcatt tgaactttta acttttcagc 17341 tgtttgtttt gactttgcat aaatactgag actagctagg aaatgagctt ttgtttcatt 17401 tgctaaaaat gatttttttt tcatgaggca atttcaagca atttgaattt agcaatctct 17461 acttgtctga aaagaagtta ttaattaaaa aaaaacaaac aaacaggaac ctattatgct 17521 ggctagtcac atgaaagaat ccaaatgaac acagtcaagg ctgaatcaac acatgaaatt 17581 attttctaat tccccaaatt acatttatgc taattagtat gtagtaaaac attttaattg 17641 tttccaatgt taaacattac agaaataatt ttgatgtttt cctaataacc cagcctttga 17701 tcatgtttgc atttatttga ttgttgtcag tttattaaat aattaagtaa gagcaacaag 17761 ataaaatgac ttcatgagag tccttttctg taacttagaa cgtcagtagt cataggaact 17821 gcggtcgttc ccttaccttt cagaagtagg ctaaagagtt gaacgcaaag gtggtttttc 17881 gaatcttcct gatccttaag cccccactag gtttatattc tatatttgat atcggattcg 17941 aacccctcga gcttaggatc cggaaccctt aatataacta agttattagg tccctcgaag 18001 aggttcacta gtactggcca ttgcggccgc ttcgaggctg cctcgcgcgt ttcggtgatg 18061 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 18121 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 18181 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 18241 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 18301 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 18361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 18421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 18481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 18541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 18601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 18661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 18721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 18781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 18841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 18901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 18961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 19021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 19081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 19141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 19201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 19261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 19321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 19381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 19441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 19501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 19561 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 19621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 19681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 19741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 19801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 19861 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 19921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 19981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 20041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 20101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 20161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 20221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgg //