LOCUS Exported 875 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Bacterial expression of Pfu DNA polymerase. ACCESSION . VERSION . KEYWORDS pET16B.Pfu SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 875) AUTHORS Goldman S, Kim R, Hung LW, Jancarik J, Kim SH TITLE Purification, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus DNA polymerase. JOURNAL Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8. PUBMED 9757114 REFERENCE 2 (bases 1 to 875) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..875 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 52..71 /label=T7 /note="T7 promoter, forward primer" promoter 52..70 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 71..95 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 110..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 145..174 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 187..198 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac 61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact 121 ttaagaagga gatataccat gggccatcat catcatcatc atcatcatca tcacagcagc 181 ggccatatcg aaggtcgtca tatgatttta gatgtggatt acataactga agaaggaaaa 241 cctgttatta ggctattcaa aaaagagaac ggaaaattta agatagagca tgatagaact 301 tttagaccat acatttacgc tcttctcagg gatgattcaa agattgaaga agttaagaaa 361 ataacggggg aaaggcatgg aaagattgtg agaattgttg atgtagagaa ggttgagaaa 421 aagtttctcg gcaagcctat taccgtgtgg aaactttatt tggaacatcc ccaagatgtt 481 cccactatta gagaaaaagt tagagaacat ccagcagttg tggacatctt cgaatacgat 541 attccatttg caaagagata cctcatcgac aaaggcctaa taccaatgga gggggaagaa 601 gagctaaaga ttcttgcctt cgatatagaa accctctatc acgaaggaga agagtttgga 661 aaaggcccaa ttataatgat tagttatgca gatgaaaatg aagcaaaggt gattacttgg 721 aaaaacatag atcttccata cgttgaggtt gtatcaagcg agagagagat gataaagaga 781 tttctcagga ttatcaggga gaaggatcct gacattatag ttacttataa tggagactca 841 ttcgacttcc cacatttagc gaaaagggca gaaaa //