LOCUS       Exported                 641 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Theta replicating shuttle vector for E. coli and gram positive 
            bacteria. High copy number in gram positives. Erythromycin 
            resistance, recommended to use BHI agar plates for E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    pTRKH2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 641)
  AUTHORS   O'Sullivan DJ, Klaenhammer TR
  TITLE     High- and low-copy-number Lactococcus shuttle cloning vectors with 
            features for clone screening.
  JOURNAL   Gene. 1993 Dec 31;137(2):227-31.
  PUBMED    8299952
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    113..134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        149..179
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    187..203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     192..214
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     211..227
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     211..227
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             223..465
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSISRSRARESLAAGRRIPGNSLAVVLQRRDWENPGVTQ
                     LNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     primer_bind     complement(306..323)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(306..322)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(315..337)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aatatttcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat
      241 tcatcgatat ctagatctcg agctcgcgaa agcttggctg caggtcgacg gatccccggg
      301 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      361 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagaatcg atacgatttt
      481 gaagtggcaa cagataaaaa aaagcagttt aaaattgttg ctgaactttt aaaacaagca
      541 aatacaatca ttgtcgcaac agatagcgac agagaaggcg aaaacattgc ctggtcgatc
      601 attcataaag caaatgcctt ttctaaagat aaaacgtata a
//
