LOCUS       Exported                 741 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Express gene of interest under CB promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-CB-IRES-copGFP-T2A-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 741)
  TITLE     pCDH-CB-IRES-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(28..627)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(111..131)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     608..627
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(628..681)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atccagaggt tgattgtcga cgaattctca ggcaccgggc ttgcgggtca tgcaccaggt
       61 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
      121 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
      181 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
      241 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
      301 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
      361 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
      421 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
      481 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
      541 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
      601 gcgcaccgtg ggcttgtact cggtcattgg gccaggattc tcctcgacgt caccgcatgt
      661 tagcagactt cctctgccct ctccactgcc aagcttgcga gatccggtgg agccgggtcc
      721 ggcggtgccg tccacggcag a
//
