LOCUS Exported 19512 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of the eGFP reporter gene was not detected in the brain and eye.. ACCESSION . VERSION . KEYWORDS pEMS1180 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 19512) AUTHORS Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM TITLE A regulatory toolbox of MiniPromoters to drive selective expression in the brain. JOURNAL Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PUBMED 20807748 REFERENCE 2 (bases 1 to 19512) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..19512 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" regulatory 3246..3255 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3252..3968 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3252..3968 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3297..3318) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3558..3577) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3905..3926 /label=EGFP-C /note="EGFP, forward primer" CDS 3981..3998 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" CDS 4011..4031 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4035..4055 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4059..4079 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 4086..4119 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 4242..4323 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4279..4298) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11968..11989 /label=F1ori-F /note="F1 origin, forward primer" promoter 12152..12256 /gene="bla" /label=AmpR promoter CDS 12257..13117 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(17324..17346) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 17432..17572 /label=bom /note="basis of mobility region from pBR322" primer_bind 17487..17506 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(17587..17604) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(17758..18346) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(17838..17857) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(18517..19377) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(19378..19482) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcctt gtggaagcta caattcaaga ggagatttgg gtgggtacac 181 agtcaaacca tgtcagcctg tgactgcttt gacctacaga acatgacaga agtcatgtgc 241 cagttccagg cttagccttt aagaggctgc cctttgcttc ctcctgcttg gaacacagcc 301 accatactgg gaggaagtcc aagcagctac atggaggaga atgaaggccc tggccactgt 361 ctccagctga ggcctgagcc tccaagcagc actcattcac cagccatgag tctgcttcct 421 tgaaagtgga tctgccatgc ctggaggggc caccccaagt gatgctgcat ggagcagaaa 481 tgagctatcc ccacccatac cgcaaaatca tattgtttta cgccactaag ttttggggca 541 atttcttatg tggcaataaa tacccgaaac attgctcgtg ggaggctggg agggagaact 601 ttgccaggct ctgtggaccc tgagctgggg cctgggcagt ggctgggtga agcctagtgc 661 aggtttgaca gctgttcccg tgttttgttt cctgcgaaaa tcacagcaac ctccttccca 721 ccctcgcccc cactgcaaac agtgttcagt ctccctggtg atgcagcccc actcggtggc 781 cagctgctgg gcacggggct gccgttcgga cagcttgcaa gacagctgtg ctcccggaaa 841 tggagggccc tggcgatggt aattggcaat gtccttcctt tgggaagtca gcctgattct 901 tcaaatacaa taacagggtt tgagaattta atcttccccc cagggatact atttattctg 961 ttattcatgc atcacggaga agaaagctct taccactaag gatctgtcaa cttgattttc 1021 tcttaatcag cgcttatgtc catctttctg ccagatctat gtcttctttg acaaaaacat 1081 ggagcatagc agttcttttc ctcagcacaa cctagacccc aaatactttg tgagtcgagg 1141 ttgtgccact gcactccagc ctgggcaaca gagcaagact ctgtctcaaa aaaaaacaca 1201 aaaaaaaata tgttgggtcc ctgagaagca ggtcagagct tttcctgcac agctcaggcc 1261 tccaggtgga agcacccctc tctcttccca ctgcccctcc ttgtggcccc ccagctgcct 1321 gtcctcctcc tgcacaccca catcctggta gccagacggg ttcccatatg gccctgggtg 1381 ctgctgatgg gcctgactca gggccaggaa acaggggcag ctgtccaggg cctgtcgctg 1441 agtcacctac ttcccgcccc cacccctccc aactgcaggc gtcagcctct ggcaaggcca 1501 ttagcagccc ctcttcctcc aggctgagct acccagaaaa cccccagctg gatgagtcac 1561 ccagggcaca ggtggattca cggactcccg gctgtgaccc tgcctctgac cagcaaggag 1621 gtccctgagg aagctcatct cccatgggat gagttcacca aggagcagga ggggaggcta 1681 catggtgggg ggcggggctg ggggctggga aggagaggct tccctggcac agagcccagg 1741 gcagtgaagg agcgggccgg gggtggtctg ttggagaaag tgaatagttg ttataccgta 1801 ttgtttagga aataatgaca aggaaaaaag tctgtacgta ttcgtacaga gaaacccatc 1861 ctaggcctac ctacaaggac ccagtccaca gtgggttgaa cccacagcca gggaactcac 1921 agatacagag gggccaactg taatgggtct ccgtgagggt ctaactccgc aagggcaggg 1981 accctgctgc tgatttcacc actgcagctc cactgccagg caatggacgg ttgagctccc 2041 gtgcagtggg catggcccag gtgacccttg acccttgctt gagttctcag cttcagggtg 2101 gtttttcctg gtggtggaca gcgcttctaa ggaagatggg ctggctcgaa ggcagcgggg 2161 gacatcaggc tttctgcttg atttcgatta ctctaatggt gacccacttt gtctgctgca 2221 cactgggctg tcttcaagat gacaggttga caatgactgc cattgggtca ttactgagca 2281 aagagccatt agcacactgg ctgtggttcc agcacagtct gtgccgggag gcaggtgtct 2341 gtcatcccag gtgtgctggc ctcatttgtc tgaaggaatc tgccgacctc aggaaacccc 2401 agcacgcctt cagaccactg ccttgcttgt ctgtagcctt cccatggtca ccgcacttcc 2461 gggccagggt cccagccagg gtttataccg agacccactc acccagacta agcgggaagc 2521 aagacggtgg gtgtcatccc acgtggaaaa agaacttagg gacctggagg aatcaggcat 2581 gggcatgttt attttgaata catgtgtcta attattcaat ggagctttct taaatgaatt 2641 caaaatacat gtgaagattt gtttccagtt gcttaatgtc tttttaatag ggaggctctg 2701 accaggagca gtggggttga cgccacagcg atgacttggg ccacgggtct gggggctctc 2761 ccgcggggcg attgggctct gtctttcggg attagggtct ctcccacagg gtttggggtt 2821 ctctctgcag atttggggga gtctctcccg cgggatgagg gggggctctc cgccgcaccc 2881 gcctatgatg gcccagagga cccgagctcg ggtcactccc gtcgccttcg gggctcggcg 2941 agggtgcagg aggcgggcgc tgagccggtg acgcgactcc gggcggctcc cgggcgcgcc 3001 gcatatatag cagcggcggc ggtggcggcg gccacaccgg gcggcggaca cgtggaggga 3061 cccggcccgc gccttctgcc cctgctgccg gccgcgccat gcggtgagcg ccccaggccg 3121 ccagagccca cccgacccgg cccgacgcct ggacctgccg cccagacccg ccaccgcacc 3181 cggaccccga cgctccgaac ccggcgcgcc gaagttccta ttcttcaaaa ggtataggaa 3241 cttccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 3301 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 3361 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 3421 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 3481 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 3541 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 3601 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 3661 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 3721 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 3781 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 3841 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 3901 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 3961 tgtacaagtt atttctgaac tgctgcccgg gctgctgcat ggaaccggat ccaaaaaaga 4021 agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtaa 4081 tataagaagt tcctattctc tagaaagtat aggaacttca gcggccgcga ctctagatca 4141 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 4201 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 4261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 4321 tgcgaattct cgtaaaactc ttcatggcag tagttattat tctctctctc tctctttttc 4381 ttttttcttg agacaggata tttctctgtt gcccaggctg gagtgcagtg gcacagtctt 4441 ggctcactgc agcctggacc tcctgggctc aagccatcct cccacctcag cctcccaagt 4501 agctggggct acaggcacat ggccaccagg cccagataat ttttcatttt tgtagagact 4561 gagtctcacc atgttaccca ggtttattat tctcattttt tagatgaaga gactgaggtc 4621 cagagaagct caatgacttg cctagtttta caaatctcct gccatcacat acccctcagc 4681 gtccttaata agagggaggc caccaactat gtgctgggca ctgtggtgga tgctggagct 4741 atagggttga gtatataaga aatggtgttg ctggagcaac tgttgcttgc ttacctgacc 4801 tatctgagaa ttaattagca ggggaacata tttttgtttt cagattcaat ataagaactt 4861 gtgtgggcaa aaataaagat cagtagtaat aacagtagtt cccatttgct gactgtactg 4921 tcctaagtgc atatatatat acatacacac acgcatacct atactcctct aatactcaaa 4981 atgatcctgt ttatgtattg ttaatatgct cattttattt ttaaattttt atttattttt 5041 atttttattt atttttgaga cggagtctca ttctgtcgcg gaggctgaag tgcagtggtg 5101 cgatctcagc tcagtgcgac ctccgcctcc cgggttcaag tgattctcct gcctcagcct 5161 ccggactagc tgggattaca ggcgcccgcc tccacgccca gctaattttt gtatttttag 5221 tagagatggg gtttcgccat gttggccagg ctggtctcgt actcctgacc ttgagtgatc 5281 cacctgcctc ggcctcccaa agtgctggga ttacaggcat gagccaccgc gccgggctaa 5341 tatgctcatt ttagtgaggc aaaaatagag gctcagagtc tgatttgtac aaaactacag 5401 agcagttaag tgtcctctca gatgtgtacc ctgatctggg tgactctagg actctaggtc 5461 tcaactgtta caaccagtta agggtttggg gaagcactgg gccaagagtc aggaaaatgg 5521 aagccacagg tagtgcaagg tcttgggaat gggacgtctg gtccaaggat tcacgcgatg 5581 actggaaccc gaagagccgg ggcccggttt acggccgcca tgaagcaacg cgcgccggta 5641 ggtttgggaa tcagggagcc ctctgaatag gagactgagt tgggagggaa aggggcttcg 5701 ctgggggagc ctcggcttct tctgggagaa aattcccacg gctacctagt gagcctgcaa 5761 actggtaggc gccggcgtag gcgcgcgggc ggggccgggg gcggggcctg cggggcgtgg 5821 cggggcgggc agagggcggg gcctgcttct cctcagcttc aggcggctgc gacgagccct 5881 caggcgaacc tctcggcttt cccgcgcggc gccgcctctt gctgcgcctc cgcctcctcc 5941 tctgctccgc caccggcttc ctcctcctga gcagtcagcc cgcgcgccgg ccggctccgt 6001 tatggcgacc cgcagccctg gcgtcgtggt gagcagctcg gcctgccggc cctggccggt 6061 tcaggcccac gcggcaggtg gcggccgggc cctgaggcgc gggatcgatc catgacaccc 6121 ccccgccccc catatctcac tataattcac tgtcaaatgg cttttatgat ttggagtttg 6181 gggatacttt taaagacagg gtcttggtag ctcctagatg gcttgggatt cacttagtaa 6241 cccttgctgg cctaaaattc aggaacttct acctcatctt cccaagtact gggttagtag 6301 ggatgtccag catatttagc ttgctttaat gattttgctg tacttttttc tgtgtgtaca 6361 ggtgcatgta tatttataaa actaacataa tatattttac ccccaaagta ccataaccct 6421 aattaatgct attaaatgtt ttgtatgctg ggctctgggc atactaatat aaccaatatt 6481 atacaatgca aaacaatttt ggaatatctt ctgtaaaatt atcttattag taagttgtaa 6541 ccatctcaga cttctaagtt tgagtttgta aaggatcagt tttttttggt gacctacaca 6601 cacacacaca cacacacaca cacacacaca cacagggggg tggattctga gaatttagag 6661 aatttagatg gttaatacca gtttttattt ttattctgtg tgatatttat ttattaagat 6721 ttatttattt attacatgta ggtacactgt atctgtcttc agacacacca gaagagggca 6781 tcagatctcg ttacagatgg ttgtgagtca ccatgtggtt gctgggattt gaactcagga 6841 cctctggaag aacagtcagt gctcttaacc gttgagccat ctcaccagcc ccgtgatatt 6901 ttatttaact tactagaata tactgtgtta aaacatattt taggggattg gggagatggc 6961 ccattctgta aagtgtctgc catgtcatct tcaggacctg cgtttgtaac cccagcatgg 7021 agaagacaga gacagggaga cccctgggct tcctggcaag ctagactagc aaatttggtt 7081 aactaggttc aatgagagac tcagtctcaa aacataagaa gtgaattttt tagacccaat 7141 ttcaatctct gaactccata cgtatgcaca tgtacctgca tataaatata tatacacacc 7201 cataaacaca tgcacacaca aaaaatggtt caaaagttct taaaatgtat aagagtagaa 7261 agtgtagagc cgggagtggt ggcgcacgcc tttaatccca gcacttggga gcagaggcag 7321 gtggatttct gagttcgagg ccagcctggt ctacagagtg agttccagga cagccagagc 7381 tatacagaga aatcctgcct caaaaaacca aaaataaagt gtatatacta atctttcatg 7441 cctaaaatat ttatttttgc cacatttttc atacctcttt aatatttttc tttttctctt 7501 tgtgcttcaa aggaaatttt gtgtcatgtt ttacttttca ttcttgactc tattcagcag 7561 taagacgcag catggctttc ttagagaagt ataatctatt acaaaacggt aacatttaac 7621 tgctctacat accctcttgt agctaaattc ctctgttaaa ctaagactta ttatctaaac 7681 gtaattaaat gttgatatct tataatgctc cactttgaaa cagctgtctt tcttttgcag 7741 attagcgatg atgaaccagg ttatgaccta gatttgtttt gtatacctaa tcattatgcc 7801 gaggatttgg aaaaagtgtt tattcctcat ggactgatta tggacaggtt agtaagacct 7861 cgattgaagt tatttttaga gggtgtgtgt gtggtgtgtg tgtgtgtgtg tgtgtgtgtg 7921 tgtgtgtgtg tgtaagagag agaggagagg catgtgtgat atatatgtga agtgtattgc 7981 atgtacatgg aatccagaga aggacattag gtgtcctact ctgttgctct cttattccct 8041 tgaatcagtg tctcttgctg aacctggggc atacatactg atttggctag gctgcctggc 8101 tagcaagacc cagggatctg cttgtctctg ccccccagtg ctgacgttac aagcaggtgc 8161 aaccacatct ggtttttata tgggtactgg gggatctgaa cttagggcct gatgcgtgcc 8221 caagtgctct cacccgccaa gccacctctc cagccaaagg gttttttttc ttgtattaat 8281 tctaacttag caactgttga gtagttgatg tttgaatata agctaagcca acttattaga 8341 ataaatgttt tctatttttt acttgtatgt gtgagtgcct gcatgtatgt atgtgaagca 8401 tatgtattcc aggatgtcag aattgtgatg gattccctgg aactggagtt acagaccatt 8461 gtgaaccact gtgtatgggt gctgggaact gaactttagg tcctctgcaa aaacagtaag 8521 gatcttaact gataaactat ctttctagcc ctaaggcttt ctacttttaa agttcgtttc 8581 tctttctctc tctctctctc tctctctctc tctctctccc tctccctccc tcctcctcct 8641 cctcctcttt cccccctctc gcctcctctt tctctctccc ctccctctct ctctctcaca 8701 cacacacaca gacagagaga ggagagagaa aggaagcctt actaattaat tacctagttt 8761 tagacataac tagtgggaat ccatagtaag taaatcaaca ttcttgaaga gaggttagaa 8821 gaattggctc tcgttgtcca tcgaggctgt ataagattat tcttctgttt tctcagctta 8881 ccatacatat tttctacgta gacaatacca attcaaatac actgtacgta aagtatatgc 8941 gcacacacac acacacacac acacacacac acacacaagt tacatattgt catatagtac 9001 taaaatttat ggactttttc atattaatac atctagagct atcatgtttt ttatctttta 9061 aaaattactt tacggggcta cagagatgtc tcagcagtta ggagcactga ctgctcttcc 9121 agaggtcctg agttcaattc ccagcaacca catggtggct cacaaccatc tgtaatggga 9181 tctgatccac tcttatggtg tgtctgaaga cagtgacagt gtactcatat acatgaaata 9241 aatctttaaa aaattacttt accatcaggg tatatatata tatatatatg tgtgtgtgtg 9301 tgtgtgtgtg tatgaagtat ttgctatttg agagggtctc actgtttagc tcagtctggc 9361 ttggaacgtt ctgtatagcc cagactggcc tcaactcagc agtccccctg aaaggctctc 9421 atatgtggag attacaggta tgtgctactg tgcccagccc tacatgttac tttgtttttg 9481 aaatagggta tcaaatagct tagactggct tctaagtcag tgtgtagcta aggatggtct 9541 gagcttctga acctgctact tcaactcctg gtgtgccatc atggccagtt tgcacagttg 9601 tgagagtaga actcaggacc tcctatgtgt tactagacat gttctctacc agctgagcta 9661 catcccaatt cgcctacatt tacttttatt ttttaaaaga tttatttatg tatacagcac 9721 tctgcctgca tgtacatctg catgccagaa gagggcacca gatctcatca tacatagttg 9781 tgagccacca tgtggttgct ggggattgaa ctcagggcct ctggaagagc agtcagtgct 9841 cttaacctct gagccatctc tgtagccccc tacatttatt ttaaagatta taggctattc 9901 tattcataga tgtgccataa ctgatttaac cagatcttca ttattttgta cttagtagag 9961 tatattagag ggaatgaatc ttaaatacac attttgacac ttgctcagct ttagacaagt 10021 aaaaatcact ggtcaaggaa atggtgctta gtggatgctt ctgaactgtc aaattgtcct 10081 ccaacaagtt acatcatttt atacttccaa cgttatgtga gagttcattt ttctttaggc 10141 tcttctagta ttttatattt gagaaataaa atttaaccaa aatttgccaa tctagtaagc 10201 ttgtttttat ttgtatcttt aaattttatg atagatgtta tagtgtactc tcctctcccc 10261 tcccctcccc tctcctcctc agcaggatct tgttatgtag ccctaaggga tctaatactt 10321 actctgtagt ccaggctgtc cttcagctca tgagacacac catcatgctt ggcatgtttc 10381 ttgttctgaa aggtatctgg ttttaatttt ggttgagctg gtgtgttggt gtatatctgt 10441 aatctcagca caggcaagta gagacaagga ttatgattac agtccagcct gggctattta 10501 gagggattct ctttaatctt tcctcatgcc ccaaaatctt acctttggta tatgaaaaat 10561 agtctccact tctgcaaaat attgctttat gaagtaagaa ttaattccct tcatagagac 10621 aaggaatgtg tcctgtaaaa gtttaatgtg taagaagtat ttgttataaa agataaatat 10681 tcagaatctt ctttttaatt cctgatttta tttctatagg actgaaagac ttgctcgaga 10741 tgtcatgaag gagatgggag gccatcacat tgtggccctc tgtgtgctca aggggggcta 10801 taagttcttt gctgacctgc tggattacat taaagcactg aatagaaata gtgatagatc 10861 cattcctatg actgtagatt ttatcagact gaagagctac tgtgtaagta taattaactt 10921 ataattaaaa aaatagggcc attctagttt tatctatatt ttttttaaac ttgtgcaaac 10981 tatgctacat aatcaactta cttgattatg taatatatat agtaatagtt ggaattacta 11041 ttattacata tttatttttc tgtgctgggg tagaacccag ggaactaagt actgtaataa 11101 ccgagcttca ttcatcctag tcatttttaa tttttttttt tttttttgtt ttttcgagac 11161 aaggtttctc tgtgtagccc tggctgtcct ggaactcact ctgtagacca ggctggcctc 11221 gaactcagaa atctgcctgc ctctgcctcc cagtgctgag attaatgacg tgcaccacca 11281 ctgcctggca tttttttaaa gttttaattt gagacccttt ctcactaact tgctcacact 11341 atcctcgaac tcattacgtg ggctggtcta gaaatttcag tcaacttgcc tcagcttctc 11401 aagtagctgg gattacaggc aggtgctacc acttctggcc atgatcattg ttgttgttgc 11461 tattattatt attattattt cactgatact tcatatcaca acacagaata gtaactgtag 11521 ttcatgacca acaaaaaagt tgtacagttt ctctgttgtc ttattttgat ctggattttg 11581 atttgtttca tggcactggg caggctaatc tcaaactgca tggctcaaat agtccttctg 11641 tcttaatctc ccatgttctg gaagtatagg tatcactaca cctagctgta ttttcatatt 11701 ttaaaaaaaa cgcctaagaa ttgctattga atgaggcgga gagatggctc agcagtcaca 11761 agtacttgta gaggactggg gtttggttac tagcacctca aagatggctt acaattatct 11821 gtagctccaa ttccagagga tctgtgtggt aaagctgcat gtatatgggc atgcaggtaa 11881 aactgctaaa catataaaaa tagctcagtc ttaagagaat tggtcgactt tttcgccctt 11941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 12001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 12061 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 12121 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 12181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 12241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 12301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 12361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 12421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 12481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 12541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 12601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 12661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 12721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 12781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 12841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 12901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 12961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 13021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 13081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 13141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 13201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 13261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 13321 cttgcaaaca aaaaaaccac cgctaccagg tcgagtaggg ataacagggt aatggatcct 13381 cgttgggcac ctgcactcat gtacgcaggc ccacatatag atgtacacac gcatatcatt 13441 caaaatagaa attatttata caagtagtgc gttggagagc tggctcagga gttgttaatg 13501 cttgctaccc ttgcaaagga cccaagttca atttccagca cctgtgttgg gcatctcaca 13561 accacatgta aatctagttt caggggattt cacccctctt ctgaactctt caggcactta 13621 tacccacaaa tggacatctc cacatagaga cacatacttt taaaaaagta atctagggtc 13681 tagagaaatg gttatagacc acggactggg atcagttccc agaagccaca tcctgtggct 13741 cacaattgcc tgtgaatcaa gttctagatc tggtggcctc gtctagcctg cagtcatcaa 13801 caaacacatg cacacatcca tacacacata atttaaaaga aagcgaaagt aacctataat 13861 gctaaacaaa gcacattctc agtgtagact gcagtggtga tgaaccttga aaatatatta 13921 atagaagcca gacacgtaag tcacaaatgg tataactcaa tttatatgaa attcccagag 13981 agtccaaagt taaaaaaaac aaaaaggcag attattggtt gtcagtagct acagagatgg 14041 gacattgaag atattaatag gtatagggtt gaggattcat ggcctttccc tcttccatgt 14101 tagcatgtct attgttgtta accttattca actcatgtta gggcaatcat attgatgaga 14161 cttaatgagt atagcttgac attcctagaa gacagagtct cacatcagac tctctgatcc 14221 tctgactctt agaatctttc aacaccctct tctgaaatga tctctgagcc tttggttgtg 14281 actgaattat gagaaagaat tttatgttga atgaatttta cttcagcatt tttacaaaac 14341 tgaagctggg gatggcgttt catgcgtgca gtccctacca tttgcctata agcctggagg 14401 aggcagaagt agaattaggc ttttcaggga catcttcagc tgcatcatga gcttgaggac 14461 aacttgagct acataagatt cccatcccca cccccacaca cacacacaca tctagtatgt 14521 acctagtatg cagtcttttc atatgtttaa aatgtagaaa ggtatatgtg aagtctccct 14581 tttccccaca acttagtccc tccaaagtat tgcatcagtt tccttgtaca tgattttatg 14641 catatataag atacactatt tctctctctc tctctctcct tccctccttc cctccaacct 14701 ctgggtatga gttgagggtt atgtatgttt caagttgtaa taagaattgc cactcaaaca 14761 tttcatccca ttttaagtta agtttgtctg attttccact ttagaagggc agccatgatg 14821 gctcatgcct ataatcccat cctagcactt gtaagcctaa tacataagac tggaggattg 14881 tcatcagctt gaggctagcc tggtctacct aatgagttcc agccagccaa gagtagaata 14941 taagaccctg tctccaaaaa atgtaccaaa ggggctggag gggtaactca gtggttaaga 15001 acagtagctg ctcttccaga ggacctgggt tcaattccca gcacccacat ggcagcttgc 15061 aactatctgt aactgtccag ctttgggata tccgacacca ttataccaat tacataaaat 15121 gaaaaaattg taaaatgtgc taaaatgagt ggggaggggg agcatggtgt ggttctgtgg 15181 gcttgggatt ttgccatgaa tccatgacta tatctcagca atatgtggat tatttctgga 15241 aacctgtggg gcatgggtgt atgggaagag gggagagaga gagagcccgc atccagccag 15301 agttcctgtg ctctgggcag gcggacgtgg gaggactgct ggacgatttc cacttggccc 15361 tgggtgggca tctggctgtg tgaagccact gaccccactc agcggggggt agataagggg 15421 catttcccat accaggttct cagactccag cattccacgc tggatacagc agaagagctg 15481 agaacagaat aggggcccca gggagacact ctcgtcccag agataaagaa ggcagaggga 15541 gagagattcc tacgcaggcg agaggcctta gtctgggcct tgatgaacag agacggtcta 15601 tggttttaga gctttattgt agaaaggcag ggggaaagag agaaggtaga aagagagaga 15661 ccagccatgg ccaagaggag agaagggggg aaaggagaga gagaagaaag gctagagagt 15721 aagaaaggtt aaagagacag aagtaaaagc ttagagtaag agagagagag agagcaagag 15781 ggagagtaag aaaagtaaaa gcttaagaga gcgaggaggg gccagcccct ctaatagtgg 15841 gcttgttatc ttgttgttgc taggtaactg tgaagcgtct agcctgaagg ccagaagctt 15901 gggacattgt ctatgtgact gaaagccatg agcctctact gtgggggctg tggaggtggt 15961 aacttggaca ggagccaggg gttccaggag acatgaggga atgccttctg tcccatgtag 16021 gtaggaatta ccaccaccgg ctttcaccgt agttcactgg agactgtttg tacatagccc 16081 attgccccac agaaaccagg cactcactgt ttcatttgca taagaattag aaggctttat 16141 tttggctggg attggtgact tgccccagaa attccagctt ttgggaagcc gaatcctgga 16201 gaaccaggag ttcaaggtca tcctcaggta cctcatttgt tcaagaccag cctgtgctac 16261 catgagacct tgtccctact cccctcaacc ccaaaacacg ctgttttcat tttataggtt 16321 gtactaacca tggaggatgg tcatttgcct ttctcttgat ttcagaagcg tagcgcgggc 16381 tttgcaccat gatctagtgt ttccagatag gcttgcctct ccacagccag gcttatcctg 16441 cttcagtccc atgtttggca agggcaacat atgactgaag aacttgcata gccttatggt 16501 gtatcatgac tcttagcccc agtttataac tgtctaacag ctggtggctt ttgtgaaaaa 16561 ggaaagcatc atttgaactt ttaacttttc agctgtttgt tttgactttg cataaatact 16621 gagactagct aggaaatgag cttttgtttc atttgctaaa aatgattttt ttttcatgag 16681 gcaatttcaa gcaatttgaa tttagcaatc tctacttgtc tgaaaagaag ttattaatta 16741 aaaaaaaaca aacaaacagg aacctattat gctggctagt cacatgaaag aatccaaatg 16801 aacacagtca aggctgaatc aacacatgaa attattttct aattccccaa attacattta 16861 tgctaattag tatgtagtaa aacattttaa ttgtttccaa tgttaaacat tacagaaata 16921 attttgatgt tttcctaata acccagcctt tgatcatgtt tgcatttatt tgattgttgt 16981 cagtttatta aataattaag taagagcaac aagataaaat gacttcatga gagtcctttt 17041 ctgtaactta gaacgtcagt agtcatagga actgcggtcg ttcccttacc tttcagaagt 17101 aggctaaaga gttgaacgca aaggtggttt ttcgaatctt cctgatcctt aagcccccac 17161 taggtttata ttctatattt gatatcggat tcgaacccct cgagcttagg atccggaacc 17221 cttaatataa ctaagttatt aggtccctcg aagaggttca ctagtactgg ccattgcggc 17281 cgcttcgagg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 17341 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 17401 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 17461 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 17521 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 17581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 17641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 17701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 17761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 17821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 17881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 17941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 18001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 18061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 18121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 18181 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 18241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 18301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 18361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 18421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 18481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 18541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 18601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 18661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 18721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 18781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat 18841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 18901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 18961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 19021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 19081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga 19141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 19201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 19261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 19321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 19381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 19441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 19501 gccacctgac gg //