LOCUS Exported 3992 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter. ACCESSION . VERSION . KEYWORDS pICPIS-CB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3992) TITLE plasmid for first generation adenovirus JOURNAL Unpublished REFERENCE 2 (bases 1 to 3992) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3992 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(24..41) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(24..40) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(33..55) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 181..636 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(268..287) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 478..499 /label=F1ori-F /note="F1 origin, forward primer" promoter 714..818 /gene="bla" /label=AmpR promoter CDS 819..1679 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1037..1056) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1850..2438 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2339..2358 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2592..2609 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2726..2747 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2762..2792 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2800..2816 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2805..2827 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2824..2840 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2824..2840 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 2858..2876 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 2858..2875 /label=SP6 /note="SP6 promoter, forward primer" intron 3580..3676 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" polyA_signal 3804..3885 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3841..3860) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3895..3914 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3990..17) /label=T7 /note="T7 promoter, forward primer" promoter complement(3991..17) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac 661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa 721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac 2881 tcaagctatg catggcgcgc ctaactataa cggtcctaag gtagcgacgc gccgaacgcg 2941 tggtacctct ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 3001 accccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 3061 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 3121 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 3181 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct actcgaggcc 3241 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 3301 attttttaat tattttgtgc agcgatgggg gcgggggggg gggggggggg ggcgcgcgcc 3361 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc 3421 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc 3481 ctataaaaag cgaagcgcgc ggcgggcggg agcgggatca gccaccgcgg tggcggccct 3541 agagtcgatc gaggaactga aaaaccagaa agttaactgg taagtttagt ctttttgtct 3601 tttatttcag gtccccgatc cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg 3661 ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg 3721 tacccgcggc cgatccaccg gtcgggatcc gctagcgttt aaacgcggcc gctcgagaat 3781 tctatcgata agtaagtgac tagaacttgt ttattgcagc ttataatggt tacaaataaa 3841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 3901 tgtccaaact catctgcacg cgtggcgcgt atctatgtcg ggtgcggaga aagaggtaat 3961 gaaatggcag gcgcgccggg cccaattcgc cc //