LOCUS Exported 1118 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion. ACCESSION . VERSION . KEYWORDS pCRISPRyl SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1118) AUTHORS Schwartz CM, Hussain MS, Blenner M, Wheeldon I TITLE Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica. JOURNAL ACS Synth Biol. 2015 Dec 29. PUBMED 26714206 REFERENCE 2 (bases 1 to 1118) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1118 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 4..251 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind 217..235 /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" CDS complement(262..282) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 cttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat 61 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt 121 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac 181 tccttccttt tcggttagag cggatgtgga gggagggcgt gaatgtaagc gtgacataac 241 taattacatg aggctagctt acacctttcg cttcttcttg gggtcggctc gagagtcgcc 301 gcccagctga gacaggtcga ttcgggtctc gtacaggccg gtgatagact ggtggatcag 361 ggtggcgtcc agcacctcct tggtagaggt gtatcgcttt cggtcgatgg tggtgtcgaa 421 gtacttgaag gcagcggggg ctcccaggtt ggtcagggtg aacaggtgaa tgatgttctc 481 agcctgctct cgaatgggct tgtctcggtg cttgttgtaa gcagacagca ccttatccag 541 gttggcgtca gccaggatca ctcgcttaga gaactcagag atctgctcga tgatctcgtc 601 caggtagtgc ttatgctgct ccacgaagag ctgcttctgc tcgttatcct cgggagagcc 661 cttcagcttc tcgtaatggg aggcgaggta caggaagttg acgtacttgg agggcagggc 721 cagctcgttt cccttctgca gctcgccggc agaggccagc attcgctttc gtccgttctc 781 cagctcgaac agagagtact tggggagctt gataatcaga tctttcttca cctccttgta 841 tcccttggcc tccaggaagt caatggggtt cttctcgaaa gaagatcgct ccatgatggt 901 aatgcccagc agctccttca cagacttcag cttcttagac ttgcccttct ccaccttggc 961 caccaccagc acagagtaag ccacggtggg agagtcgaaa cctccgtact tcttggggtc 1021 ccagtccttc tttcgggcga tcagcttgtc agagtttcgc ttgggcagga tggactcctt 1081 agagaaacct ccggtctgca cctcggtctt cttcacga //