LOCUS       Exported                6470 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of mutated L. gasseri gusA from strong Ppgm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pTRK892
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Duong T, Miller MJ, Barrangou R, Azcarate-Peril MA, Klaenhammer TR
  TITLE     Construction of vectors for inducible and constitutive gene 
            expression in Lactobacillus.
  JOURNAL   Microb Biotechnol. 2011 May;4(3):357-67. doi: 
            10.1111/j.1751-7915.2010.00200.x. Epub 2010 Sep 1.
  PUBMED    21375708
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(5002..5013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 taaaagcccc ctgacgaaag tcgaaggggg tttttatttt ggtttgatgt tgcgattaat
       61 agcaatacaa ttgcaataaa caaaatgatc ttccttcagg ttatgaccat ctgtgccagt
      121 tcgtaatgtc tggtcaactt tccgactctg agaaacttct ggaatcgcta gagaatttct
      181 ggaatgggat tcaggagtgg acagaacgac acggatatat agtggatgtg tcaaaacgca
      241 taccattttg aacgatgacc tctaataatt gttaatcatg ttggttacgt atttattaac
      301 ttctcctagt attagtaatt atcatggctg tcatggcgca ttaacggaat aaagggtgtg
      361 cttaaatcgg gccattttgc gtaataagaa aaaggattaa ttatgagcga attgaattaa
      421 taataaggta atagatttac attagaaaat gaaaggggat tttatgcgtg agaatgttac
      481 agtctatccc ggcaatagtt acccttatta tcaagataag aaagaaaagg atttttcgct
      541 acgctcaaat cctttaaaaa aacacaaaag accacatttt ttaatgtggt ctttattctt
      601 caactaaagc acccattagt agatcttatt aagattaccg ttatccgtga aaaacgagtg
      661 gtagcaattg ctaaataaca aaaagagtat gagtttttgc tcatactctt tttgttattt
      721 gtgcaaatac cgctctactt gtataattag aacaagtata taaggaaagt agccgaatat
      781 gtttaaaatt attgttgaat tattcttatt agtaattatt tcagctgcaa ttacgccatt
      841 tattagagat ctaatcgatg catgccatgg tgcgacaagt aataaactaa acaaaacaac
      901 tacaaaatat ttctttttgt ttttcatgat ttttacactt ctcttagtat gcttttgtta
      961 taagttagca caaaaaagca gaaaataaaa agtagaaata aaaaaagatg tttttttgcc
     1021 catatctcta tgaaaaaaac tgtgaaatgt gtaaaatatg gatgaaacat tgaatttaaa
     1081 aggagatatt tcatgtcaaa attagtttta atccgtcacg gtcaaagtga atggaacctt
     1141 tcaaaccaat ttactggttg ggttgacgtt aacctttcag aaaaaggtgt tgaagaagct
     1201 aagaaggctg aattctacta gaaaggaaaa tcatctatgg aatctgcact atatccaatt
     1261 caaaataaat atcggtttaa cactttaatg aatggcactt ggcaatttga aactgatcct
     1321 aactctgttg gtcttgacga gggatggaat aaagagttgc ctgatcctga agaaatgcct
     1381 gtaccaggta cgtttgcaga attaactact aagcgagacc gtaaatacta tactggagac
     1441 ttttggtatc aaaaagactt ctttattcct tcatttctaa agaagaaaga actttatatc
     1501 cgttttggtt cggttactca tcgcgcaaaa gtatttatta atggacatga agtcggtcaa
     1561 catgaaggtg gttttttacc atttcaagta aaaatttcaa attatattaa ttacgaccaa
     1621 actaatcgtg taactgtttt agtcaataac gaattatctg aaaaagctat tccttgcggc
     1681 accgaagaaa tcttagataa cggtcaaaaa cttgctcaac cttattttga tttcttcaat
     1741 tattctggca ttatgcggaa tgtctggctc ttagcacttc ctcaaagcca aatcactaat
     1801 tttaaactaa attatcaatt agcaaataat aaggcaacaa ttacctacaa tatcgaggca
     1861 aataataatg ctgaatttaa agtaacactt ttcgataatc aaaaagaagt agcgtgtgct
     1921 acttctaaaa atactagtag tttaacaatt aagaatccgc acctttggag tccaaacgat
     1981 ccgtattcat acaaaataaa gattgaaatg ctcgaagacg gaaaaacagt tgacgaatac
     2041 acagataaaa ttggtatccg cacagttaaa attgtgaatg ataaaatctt gctcaataat
     2101 cacccaattt atttaaaagg ctttggcaag cacgaagatt ttaatgtttt aggcaaagca
     2161 gttaacgaaa gcattatcaa acgcgactac gaatgcatga aatggattgg cgctaactgt
     2221 tttagaagca gtcactatcc ttacgccgaa gaatggtatc aatatgccga taaatatggc
     2281 tttttaatta ttgatgaagt acccgctgtt ggtcttaatc gttcaataac taactttctt
     2341 aatgtaacta attctaatca gtcgcacttt tttgcttcga aaactgtgcc tgaattaaaa
     2401 aaggtccatg aacaagaaat aaaagaaatg atcgatcgcg accagcgtca cccttcagtg
     2461 attgcctgga gtttattcaa tgaaccagaa tcaactactc aagaatccta tgactatttc
     2521 aaagatattt ttgcctttgc gagaaaattg gatccacaaa atcgtcctta tactggaact
     2581 ttagttatgg gtagcggtcc aaaagtggat aagcttcacc cactttgtga ctttgtctgc
     2641 ttaaaccgtt attatggttg gtacgttgct ggtggtcctg aaatcgttaa tgctaaaaag
     2701 atgctggaag atgaactaga cggctggcaa aacttaaagc ttaataaacc atttgtcttt
     2761 actgagtttg gcgctgatac attatcttct tctcatcgcc ttccagatga aatgtggagc
     2821 caagaatatc aaaatgaata ttatcaaatg tattttgata tatttaagaa atatccattt
     2881 atttgtggcg aattagtttg gaactttgct gactttaaga cgagtgaagg aatcatgcgt
     2941 gttggtggta acgctaaagg aatttttact cgcgatcgtg aacctaaaga tattgccttt
     3001 accttgaaaa agagatggca acaattaaat taatatttta gtttttacaa ataatcgtgc
     3061 tcaattaaaa ataatcgata tcattttagt tcatttgata tcgattattt gattatgggc
     3121 gcgatttttt attgattttg ataataattt ctaactaaga aatgtttcat taatttatga
     3181 aactaatatc ttgtttctta aacaaatcat atacaactaa gtctgatgaa tccatctaga
     3241 gcggccgctc tagaagctta tacactgata ataacatcaa gacgcaattt tatacaacgt
     3301 taaaaatatc ctttttcgca ctttaagttt gttattttaa tctaaagaaa aaagagacta
     3361 gtatactagc ctcctttttc ttcagattat ttttttccat gctctctagc atatttggcc
     3421 atctgaatac ctgcttgtcg accaaaaatc acagtttcag caatagaatt atctagattc
     3481 aggaattgtc agataggcct aatgactggc ttttataata tgagataatg ccgactgtac
     3541 tttttacagt cggttttcta atgtcactaa cctgccccgt tagttgaaga aggtttttat
     3601 attacagctc cagatcgatt cacaaaaaat aggcacacga aaaacaagtt aagggatgca
     3661 gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa
     3721 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa
     3781 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt
     3841 atacttttgt ttatctttgt gtgatattct tgattttttt ctacttaatc tgataagtga
     3901 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat
     3961 atcaacttct gccattaaaa gtaatgccaa tgagcgtttt gtatttaata atcttttagc
     4021 aaacccgtat tccacgatta aataaatctc attagctata ctatcaaaaa caattttgcg
     4081 tattatatcc gtacttatgt tataaggtat attaccatat attttatagg attggttttt
     4141 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag
     4201 tttattttct gtagttttgc ataatttatg gtctatttca atggcagtta cgaaattaca
     4261 cctctttact aattcaaggg taaaatggcc ttttcctgag ccgatttcaa agatattatc
     4321 atgttcattt aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa
     4381 gttttgactg tgttttatat ttttctcgtt cattataacc ctctttaatt tggttatatg
     4441 aattttgctt attaacgatt cattataacc acttattttt tgtttggttg ataatgaact
     4501 gtgctgatta caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata
     4561 attatagcac gagctctgat aaatatgaac atgatgagtg atcgttaaat ttatactgca
     4621 atcggatgcg attattgaat aaaagatatg agagatttat ctaatttctt ttttcttgta
     4681 aaaaaagaaa gttcttaaag gttttatagt tttggtcgta gagcacacgg tttaacgact
     4741 taattacgaa gtaaataagt ctagtgtgtt agactttatg aaatctatat acgtttatat
     4801 atatttatta tccgattttt tattaaaacg tctcaaaatc gtttctgaga cgttttagcg
     4861 tttatttcgt ttagttatcg gcataatcgt taaaacaggc gttatcgtag cgtaaaagcc
     4921 cttgagcgta gcgtggcttt gcagcgaaga tgttgtctgt tagattatga aagccgatga
     4981 ctgaatgaaa taataagcgc agcgcccttc tatttcggtt ggaggaggct caagggagta
     5041 tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat tttgccgagc
     5101 ggtagcgctg gaaaattttt gaaaaaaatt tggaatttgg aaaaaaatgg ggggaaagga
     5161 agcgaatttt gcttccgtac tacgaccccc cattaagtgc cgagtgccaa tttttgtgcc
     5221 aaaaacgctc tatcccaact ggctcaaggg tttaaggggt ttttcaatcg ccaacgaatc
     5281 gccaacgttt tcgccaacgt tttttataaa tctatattta agtagcttta ttgttgtttt
     5341 tatgattaca aagtgataca ctaactttat aaaattattt gattggagtt ttttaaatgg
     5401 tgatttcaga atcgaaaaaa agagttatga tttctctgac aaaagagcaa gataaaaaat
     5461 taacagatat ggcgaaacaa aaaggttttt caaaatctgc ggttgcggcg ttagctatag
     5521 aagaatatgc aagaaaggaa tcagaacaaa aaaaataagc gaaagctcgc gtttttagaa
     5581 ggatacgagt tttcgctact tgtttttgat aaggtaatta tatcatggct attaaaaata
     5641 ctaaagctag aaattttgga tttttattat atcctgactc aattcctaat gattggaaag
     5701 aaaaattaga gagtttgggc gtatctatgg ctgtcagtcc tttacacgat atggacgaaa
     5761 aaaaagataa agatacatgg aatagtagtg atgttatacg aaatggaaag cactataaaa
     5821 aaccacacta tcacgttata tatattgcac gaaatcctgt aacaatagaa agcgttagga
     5881 acaagattaa gcgaaaattg gggaatagtt cagttgctca tgttgagata cttgattata
     5941 tcaaaggttc atatgaatat ttgactcatg aatcaaagga cgctattgct aagaataaac
     6001 atatatacga caaaaaagat attttgaaca ttaatgattt tgatattgac cgctatataa
     6061 cacttgatga aagccaaaaa agagaattga agaatttact tttagatata gtggatgact
     6121 ataatttggt aaatacaaaa gatttaatgg cttttattcg ccttagggga gcggagtttg
     6181 gaattttaaa tacgaatgat gtaaaagata ttgtttcaac aaactctagc gcctttagat
     6241 tatggtttga gggcaattat cagtgtggat atagagcaag ttatgcaaag gttcttgatg
     6301 ctgaaacggg ggaaataaaa tgacaaacaa agaaaaagag ttatttgctg aaaatgagga
     6361 attaaaaaaa gaaattaagg acttaaaaga gcgtattgaa agatacagag aaatggaagt
     6421 tgaattaagt acaacaatag atttattgag aggagggatt attgaataaa
//
