LOCUS Exported 9368 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion. ACCESSION . VERSION . KEYWORDS MSCV-NSD3-short del(1-100) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9368) AUTHORS Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, Pappin DJ, Joshua-Tor L, Vakoc CR TITLE NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 Chromatin Remodeler. JOURNAL Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 10.1016/j.molcel.2015.10.033. PUBMED 26626481 REFERENCE 2 (bases 1 to 9368) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9368 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 579..920 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 987..1403 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1333..1355 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1371..1387 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 3066..3131 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" promoter 3148..3647 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(3183..3206) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 3580..3599 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 3668..4267 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3668..3687) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4164..4184 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 4345..4897 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4489..4506) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4716..4735 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4909..5628 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4954..4975) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5215..5234) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5562..5583 /label=EGFP-C /note="EGFP, forward primer" LTR 5730..6244 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" primer_bind complement(6413..6429) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6413..6429) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6426..6448) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6437..6453 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6461..6491) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6506..6527 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6644..6661) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6815..7403) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6895..6914) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7574..8434) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8197..8216 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8435..8539) /gene="bla" /label=AmpR promoter primer_bind 8607..8625 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8663..8685) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8785..8804 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8998..9020 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 9148..9167 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" LTR 9367..515 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" ORIGIN 1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1381 ccctcactcc ttctctaggc gccggaatta gatctaccat gtttggtgca gttagaaact 1441 ttagccccac tgactattat cattcagaaa ttccaaacac aagaccacat gaaattctgg 1501 aaaaaccttc ccctccacag ccaccacctc ctccttcggt accacaaact gtgattccaa 1561 agaagactgg ctcacctgaa attaaactaa aaataaccaa aactatccag aatggcaggg 1621 aattgtttga gtcttccctt tgtggagacc ttttaaatga agtacaggca agtgagcaca 1681 cgaaatcaaa gcatgaaagc agaaaagaaa agaggaaaaa aagcaacaag catgactcat 1741 caagatctga agagcgcaag tcacacaaaa tccccaaatt agaaccagag gaacaaaata 1801 gaccaaatga gagggttgac actgtatcag aaaaaccaag ggaagaacca gtactaaaag 1861 aggaagcccc agttcagcca atactatctt ctgttccaac aacggaagtg tccactggtg 1921 ttaagtttca ggttggcgat cttgtgtggt ccaaggtggg aacctatcct tggtggcctt 1981 gtatggtttc aagtgatccc cagcttgagg ttcatactaa aattaacaca agaggtgccc 2041 gagaatatca tgtccagttt tttagcaacc agccagagag ggcgtgggtt catgaaaaac 2101 gggtacgaga gtataaaggt cataaacagt atgaagaatt actggctgag gcaaccaaac 2161 aagccagcaa tcactctgag aaacaaaaga ttcggaaacc ccgacctcag agagaacgtg 2221 ctcagtggga tattggcatt gcccatgcag agaaagcatt gaaaatgact cgagaagaaa 2281 gaatagaaca gtatactttt atttacattg ataaacagcc tgaagaggct ttatcccaag 2341 caaaaaagag tgttgcctcc aaaaccgaag ttaaaaaaac ccgacgacca agatctgtgc 2401 tgaatactca gccagaacag accaatgcag gggaggtggc ctcctcactc tcaagtactg 2461 aaattcggag acatagccag aggcggcaca caagtgcgga agaggaagag ccaccgcctg 2521 ttaaaatagc ctggaaaact gcggcagcaa ggaaatcctt accagcttcc attacgatgc 2581 acaaagggag cctggatttg cagaagtgta acatgtctcc agttgtgaaa attgaacaag 2641 tgtttgctct tcagaatgct acaggggatg ggaaatttat cgatcaattt gtttattcaa 2701 caaagggaat tggtaacaaa acagaaataa gtgtcagggg gcaagacagg cttataattt 2761 ctacaccaaa ccagagaaat gaaaagccaa cgcagagtgt atcatctcct gaagcaacat 2821 ctggttctac aggctcagta gaaaagaagc aacagagaag atcaattaga actcgttctg 2881 aatcagagaa atccactgag gttgtgccaa agaagaagat caaaaaggag caggttgaaa 2941 cagttcctca ggctacagtg aagactggat tacagaaagg gtcggcggac cggggagtgc 3001 agggctctgt cagattcagt gacagctccg tctccgcagc gattgaggaa actgtggacg 3061 gaggcgatta caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg 3121 acgatgacaa gtgatgagaa ttctaccggg taggggaggc gcttttccca aggcagtctg 3181 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc 3241 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg 3301 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt 3361 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg 3421 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg 3481 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca 3541 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc 3601 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa 3661 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 3721 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 3781 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 3841 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 3901 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 3961 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 4021 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 4081 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 4141 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 4201 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 4261 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc 4321 atccaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt 4381 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 4441 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 4501 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 4561 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 4621 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 4681 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 4741 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 4801 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg 4861 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag 4921 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 4981 ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg ccacctacgg caagctgacc 5041 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 5101 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 5161 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 5221 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 5281 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 5341 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcg 5401 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 5461 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 5521 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 5581 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaatg aattaattaa 5641 gaattatcaa gcttatcgat accgtcgacc tgcagccaag cttatcgata aaataaaaga 5701 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc 5761 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt 5821 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta 5881 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct 5941 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc 6001 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct 6061 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac 6121 tgcgtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg 6181 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt 6241 tcatgggtaa cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt 6301 aagtaatcct gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat 6361 agttcattat ggacagcgca gaagagctgg ggagaattaa ttcgtaatca tggtcatagc 6421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 7261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8461 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8521 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 8581 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 8641 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 8701 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 8761 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 8821 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 8881 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 8941 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 9001 agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa 9061 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc 9121 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc 9181 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt 9241 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct 9301 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa 9361 ggggggaa //