LOCUS Exported 11153 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus. ACCESSION . VERSION . KEYWORDS pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11153) AUTHORS Gaglia G, Lahav G TITLE Constant rate of p53 tetramerization in response to DNA damage controls the p53 response. JOURNAL Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168. PUBMED 25344068 REFERENCE 2 (bases 1 to 11153) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11153 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" LTR 2449..2629 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2676..2801 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3294..3527 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3712..3756 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4023..4140 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4180..4200 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 4201..5379 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 4432..5370 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 5327..5347 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 5385..5409 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6592..6616) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind 7523..7544 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(7591..7611) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(7649..7669) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7654..7983 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7834..7969 /label=SV40 ori /note="SV40 origin of replication" CDS 8057..9082 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" misc_feature 9235..9823 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9288..9308) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9706..9717) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9898..10131 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 10203..10324 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10240..10259) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10294..10313 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 10343..10478 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(10510..10529) /label=T7 /note="T7 promoter, forward primer" promoter complement(10511..10529) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(10539..10556) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(10539..10555) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10548..10570) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 10696..11151 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10783..10802) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10993..11014 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaatttaa ccctcactaa agggaacaaa agctggagct gcaagcttaa 2221 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 2281 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 2341 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 2401 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg 2461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 2521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 2581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 2641 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 2701 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 2761 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 2821 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 2881 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 2941 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 3001 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 3061 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 3121 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 3181 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 3241 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 3301 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 3361 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 3421 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 3481 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 3541 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 3601 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 3661 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 3721 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 3781 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 3841 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 3901 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 3961 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 4021 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 4081 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt 4141 atcgataagc ttgatatcac catgattacg ccaagctatc aactttgtat agaaaagttg 4201 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 4261 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 4321 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 4381 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 4441 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 4501 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 4561 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 4621 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 4681 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 4741 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 4801 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 4861 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 4921 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 4981 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 5041 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 5101 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 5161 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 5221 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 5281 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 5341 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattcaagt ttgtacaaaa 5401 aagcaggctt catggaggag ccgcagtcag atcctagcgt cgagccccct ctgagtcagg 5461 aaacattttc agacctatgg aaactacttc ctgaaaacaa cgttctgtcc cccttgccgt 5521 cccaagcaat ggatgatttg atgctgtccc cggacgatat tgaacaatgg ttcactgaag 5581 acccaggtcc agatgaagct cccagaatgc cagaggctgc tccccgcgtg gcccctgcac 5641 cagcagctcc tacaccggcg gcccctgcac cagccccctc ctggcccctg tcatcttctg 5701 tcccttccca gaaaacctac cagggcagct acggtttccg tctgggcttc ttgcattctg 5761 ggacagccaa gtctgtgact tgcacgtact cccctgccct caacaagatg ttttgccaac 5821 tggccaagac ctgccctgtg cagctgtggg ttgattccac acccccgccc ggcacccgcg 5881 tccgcgccat ggccatctac aagcagtcac agcacatgac ggaggttgtg aggcgctgcc 5941 cccaccatga gcgctgctca gatagcgatg gtctggcccc tcctcagcat cttatccgag 6001 tggagggaaa tttgcgtgtg gagtatttgg atgacagaaa cacttttcga catagtgtgg 6061 tggtgcccta tgagccgcct gaggttggct ctgactgtac caccatccac tacaactaca 6121 tgtgtaacag ttcctgcatg ggcggcatga accggaggcc catcctcacc atcatcacac 6181 tggaggatag ctctggaaac ctactgggac ggaacagctt tgaggtgcgt gtttgtgcct 6241 gtcctgggag agaccggcgc acagaggaag agaatctccg caagaaaggg gagcctcacc 6301 acgagctgcc cccagggagc actaagcgag cactgcccaa caacaccagc tcctctcccc 6361 agccaaagaa gaaaccactg gatggagaat atttcaccct tcagatccgt gggcgtgagc 6421 gcttcgagat gttccgagag ccgaatgagg ccttggaact caaggatgcc caggctggga 6481 aggagccagg ggggagcagg gctcactcca gccacctgaa gtccaaaaag ggtcagtcta 6541 cctcccgcca taaaaaactc atgttcaaga cagaagggcc tgactcagac gacccagctt 6601 tcttgtacaa agtggccatg gtgtctgagc tgattaagga gaacatgcac atgaagctgt 6661 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc 6721 cctacgaggg cacccagacc atgagaatca aggccgtcga gggcggccct ctccccttcg 6781 ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc aaccacaccc 6841 agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca 6901 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct 6961 gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc 7021 agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct gacggcggcc 7081 tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact 7141 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact 7201 atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc 7261 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac agaggaggag 7321 gtggaggtgg aggtggggga gccaagaacg gcatcaaggc caacttcaag atccgccaca 7381 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 7441 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag 7501 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 7561 ctctcggcat ggacgagctg tacaagtaat caactttatt atacatagtt gataattcac 7621 tggccgtcga gatccggact agtcctgtgg aatgtgtgtc agttagggtg tggaaagtcc 7681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 7981 aaaaagcttg ccagctgggg cgccctctgg taaggttggg aagccctgca gatctctcgt 8041 ccgggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 8101 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 8161 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 8221 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 8281 ggaagtgctt gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc 8341 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 8401 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 8461 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 8521 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 8581 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 8641 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 8701 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 8761 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 8821 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 8881 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 8941 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 9001 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 9061 tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg 9121 cctcagccag caactcgcgc gagcctagca aggcatcgat aagcttgata tcgaattcct 9181 gcagcccggg ggatccacta gttaacgcgt cgaccagcta gctggccagt cgacaatcaa 9241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 9301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 9361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 9421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 9481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 9541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 9601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 9661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 9721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 9781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac 9841 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 9901 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct 9961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 10021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 10081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 10141 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 10201 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 10261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 10321 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 10381 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 10441 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgcg tcgagacgta 10501 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 10561 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 10621 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 10681 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 10741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 10801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 10861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 10921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 10981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 11041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 11101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcc //