LOCUS Exported 601 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses high specificity SpCas9. Px330-like plasmid.. ACCESSION . VERSION . KEYWORDS eSpCas9(1.1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 601) AUTHORS Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F TITLE Rationally engineered Cas9 nucleases with improved specificity. JOURNAL Science. 2015 Dec 1. pii: aad5227. PUBMED 26628643 REFERENCE 2 (bases 1 to 601) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..601 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(303..323) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(330..395) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" regulatory 395..404 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg gttcttccgt 61 ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt ttcgccgctg 121 tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt gttgcccagc 181 accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca gcccacagag 241 ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgg ctgctgggac tccgtggata 301 ccgaccttcc gcttcttctt tggggccatc ttatcgtcat cgtctttgta atcaatatca 361 tgatccttgt agtctccgtc gtggtcctta tagtccatgg tggcaccggt ccaacctgaa 421 aaaaagtgat ttcaggcagg tgctccaggt aattaaacat taatacccca ccaaccaacc 481 atcccttaaa cccttacctc ttgctcagct aattacagcc cggaggagaa gggccgtccc 541 gcccgctcac ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg 601 c //