LOCUS       Exported                7601 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses DREADDs Gq receptor under oxytocin promoter control.
ACCESSION   .
VERSION     .
KEYWORDS    AAV-mOXT-hM3Dq-mCherry-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Penagarikano O, Lazaro MT, Lu XH, Gordon A, Dong H, Lam HA, Peles E,
            Maidment NT, Murphy NP, Yang XW, Golshani P, Geschwind DH
  TITLE     Exogenous and evoked oxytocin restores social behavior in the 
            Cntnap2 mouse model of autism.
  JOURNAL   Sci Transl Med. 2015 Jan 21;7(271):271ra8. doi: 
            10.1126/scitranslmed.3010257.
  PUBMED    25609168
REFERENCE   2  (bases 1 to 7601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     protein_bind    1114..1147
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      2945..2954
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2951..3661
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3096..3114)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3364..3383
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3670..3703)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3728..4316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3781..3801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4199..4210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4348..4824
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4547..4566)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4864..5004
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4864..4993
                     /label=AAV2 ITR
     rep_origin      5079..5534
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5166..5185)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5376..5397
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5551..5570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5670..5692
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5730..5748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5816..5920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5921..6781
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6139..6158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6952..7540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7441..7460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgtcg ctcacacact aggcaagtac
      181 tctgctactg ggctgcatgt aacacccttc ccatgcagac ctatgcagac ctgcagccca
      241 aacctgaaat gtacccagag ctgcccaatt gattgcacca aaatgggcca accattatat
      301 gtggccacct gagaagggta tgaccttggc acaaatggct tgctgtagct gaggccacct
      361 gttggccaca ctccagcagc tgatggccac tgttttctca aacaggagtc taggcaccta
      421 gtgtggtagt ggataactaa actcagcatt tgggagacag aagcagatgg agcgctgtga
      481 gttcaaggcc agcctggtct acacagcagg ttctaataca gagttttaaa tactagtgta
      541 attttccttt tgctgtaatt tttctttctt tttattttaa tttggtctgt taactgtgtt
      601 ttggttttga tctctactga gactttgttt tacgggctag ttagaagagc tgaggtgcat
      661 tcagagattg aacaagacgc catctttttc cccatctaag tttcaggtcc atgtaagggc
      721 tccctcactc acttgcccta tcctgcctta ttctgagata ttggatatct gtgaaaaaca
      781 gctcctggct agggcgcacc tccaacccct cccaagtctc tctagcctct tgtagcctag
      841 gccacccctt ccaggctgct tctcttttga gttccaggtc attagcagag acgatgacct
      901 tgaccctagc ccagaccctg caaatgaagg gcctgcctct aaacagcgtg gaacaatttg
      961 acccaagaga ccttctgtga ccagtcatgc tgtcaccctc tttagacagt gctccaccat
     1021 ggcagtgccc agacataaaa aggtcggtct gggccggaga aaccatcacc tacagcggat
     1081 ctcagactga gcaccatcgc cgtcgactcc ggaataactt cgtataggat actttatacg
     1141 aagttatgct agcgccacca tgaccttgca caataacagt acaacctcgc ctttgtttcc
     1201 aaacatcagc tcctcctgga tacacagccc ctccgatgca gggctgcccc cgggaaccgt
     1261 cactcatttc ggcagctaca atgtttctcg agcagctggc aatttctcct ctccagacgg
     1321 taccaccgat gaccctctgg gaggtcatac cgtctggcaa gtggtcttca tcgctttctt
     1381 aacgggcatc ctggccttgg tgaccatcat cggcaacatc ctggtaattg tgtcatttaa
     1441 ggtcaacaag cagctgaaga cggtcaacaa ctacttcctc ttaagcctgg cctgtgccga
     1501 tctgattatc ggggtcattt caatgaatct gtttacgacc tacatcatca tgaatcgatg
     1561 ggccttaggg aacttggcct gtgacctctg gcttgccatt gactgcgtag ccagcaatgc
     1621 ctctgttatg aatcttctgg tcatcagctt tgacagatac ttttccatca cgaggccgct
     1681 cacgtaccga gccaaacgaa caacaaagag agccggtgtg atgatcggtc tggcttgggt
     1741 catctccttt gtcctttggg ctcctgccat cttgttctgg caatactttg ttggaaagag
     1801 aactgtgcct ccgggagagt gcttcattca gttcctcagt gagcccacca ttacttttgg
     1861 cacagccatc gctggttttt atatgcctgt caccattatg actattttat actggaggat
     1921 ctataaggaa actgaaaagc gtaccaaaga gcttgctggc ctgcaagcct ctgggacaga
     1981 ggcagagaca gaaaactttg tccaccccac gggcagttct cgaagctgca gcagttacga
     2041 acttcaacag caaagcatga aacgctccaa caggaggaag tatggccgct gccacttctg
     2101 gttcacaacc aagagctgga aacccagctc cgagcagatg gaccaagacc acagcagcag
     2161 tgacagttgg aacaacaatg atgctgctgc ctccctggag aactccgcct cctccgacga
     2221 ggaggacatt ggctccgaga cgagagccat ctactccatc gtgctcaagc ttccgggtca
     2281 cagcaccatc ctcaactcca ccaagttacc ctcatcggac aacctgcagg tgcctgagga
     2341 ggagctgggg atggtggact tggagaggaa agccgacaag ctgcaggccc agaagagcgt
     2401 ggacgatgga ggcagttttc caaaaagctt ctccaagctt cccatccagc tagagtcagc
     2461 cgtggacaca gctaagactt ctgacgtcaa ctcctcagtg ggtaagagca cggccactct
     2521 acctctgtcc ttcaaggaag ccactctggc caagaggttt gctctgaaga ccagaagtca
     2581 gatcactaag cggaaaagga tgtccctggt caaggagaag aaagcggccc agaccctcag
     2641 tgcgatcttg cttgccttca tcatcacttg gaccccatac aacatcatgg ttctggtgaa
     2701 caccttttgt gacagctgca tacccaaaac cttttggaat ctgggctact ggctgtgcta
     2761 catcaacagc accgtgaacc ccgtgtgcta tgctctgtgc aacaaaacat tcagaaccac
     2821 tttcaagatg ctgctgctgt gccagtgtga caaaaaaaag aggcgcaagc agcagtacca
     2881 gcagagacag tcggtcattt ttcacaagcg cgcacccgag caggccttga aggatccccc
     2941 ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3001 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3061 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3121 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3181 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3241 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3301 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3361 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     3421 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     3481 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3541 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3601 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     3661 aggcgcgcca taacttcgta taatgtatgc tatacgaagt tatgaattcg atatcaagct
     3721 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3781 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3841 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3901 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3961 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4021 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4081 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4141 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4201 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4261 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     4321 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     4381 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     4441 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     4501 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     4561 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     4621 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     4681 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     4741 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     4801 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4861 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4921 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4981 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     5041 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     5101 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5161 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5221 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5281 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5341 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5401 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     5461 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5521 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5581 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5641 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5701 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5761 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5821 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5881 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5941 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6001 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6061 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6121 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6181 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6241 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6301 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6361 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6421 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6481 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6541 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6601 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6661 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6721 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6781 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6841 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6901 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6961 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7021 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7081 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7141 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7201 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7261 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7321 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7381 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7441 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7501 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7561 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
