LOCUS       Exported                1055 bp ds-DNA     linear   SYN 23-AUG-2021
DEFINITION  LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of 
            transcriptional repression.
ACCESSION   .
VERSION     .
KEYWORDS    LEXY TR (without KRAB domain) (pDN162)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             513..566
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(1027..1045)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tccgccacca tgaaagctct gacagccagg cagcaggaag
      121 tgttcgatct gattagagac cacatatcac agaccggtat gccacctaca agggcagaga
      181 ttgcacagcg ccttggtttt cgctcaccga atgccgccga agaacacctc aaggctcttg
      241 cacgcaaggg cgttatcgag attgtgtctg gggcctccag aggcatcagg ctcctccagg
      301 aagaggaaga gggcctcccc ttggtcgggc gcgtggcagc tggtgagcca gggggctccg
      361 gaggaagtga gagcggaggc cccgctgacg ccctggacga ctttgacctg gatatgcttc
      421 ccgcagatgc cctggacgac tttgacttgg acatgctgcc cgcagatgcg ctggatgact
      481 ttgatctgga catgctgcct ggtggtagcg gcgaaggtcg agggagcctg ctaacatgtg
      541 gcgatgttga ggagaaccct ggtcccgggg ccgccgccaa gagggttaag ctcgacggcg
      601 gtagtatgaa ggcccttact gcaaggcagc aagaggtctt tgacctgatt cgagaccata
      661 tttcccagac aggtatgccc ccgacgaggg cagagattgc acagcggctg ggattccggt
      721 ccccgaatgc tgcggaggaa cacttgaagg ccctggcccg aaaaggtgtg attgagattg
      781 tctcaggagc atctcggggc ataagattgc tgcaggagga agaggagggc cttcctcttg
      841 taggacgcgt agccgctggg gaacccggag gatccggcgg ctctgtgagc aagggcgagg
      901 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      961 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1021 agaccgccaa gctgaaggtg accaagggtg gcccc
//
