LOCUS Exported 18098 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).. ACCESSION . VERSION . KEYWORDS pEMS1277 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 18098) AUTHORS Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM TITLE A regulatory toolbox of MiniPromoters to drive selective expression in the brain. JOURNAL Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PUBMED 20807748 REFERENCE 2 (bases 1 to 18098) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..18098 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 134..437 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 439..716 /label=chicken beta-actin promoter primer_bind 1659..1681 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" regulatory 1818..1827 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1824..2540 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1824..2540 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1869..1890) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2130..2149) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2477..2498 /label=EGFP-C /note="EGFP, forward primer" CDS 2553..2570 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" CDS 2583..2603 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2607..2627 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2631..2651 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 2658..2705 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." polyA_signal 2828..2909 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2865..2884) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10554..10575 /label=F1ori-F /note="F1 origin, forward primer" promoter 10738..10842 /gene="bla" /label=AmpR promoter CDS 10843..11703 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(15910..15932) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 16018..16158 /label=bom /note="basis of mobility region from pBR322" primer_bind 16073..16092 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(16173..16190) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(16344..16932) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(16424..16443) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(17103..17963) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(17964..18068) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctctgc aggcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 421 tcatcgctat taacatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc 481 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg 541 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 601 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 661 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga 721 gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 961 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 1021 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 1081 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 1141 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 1201 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 1261 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 1321 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg 1381 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 1441 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 1501 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 1621 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 1681 cggcggctct agacaattgt actaaccttc ttctctttcc tctcctgaca ggttggtgta 1741 cagtagcttc caccacgcgt ggcgcgccga agttcctatt ccgaagttcc tattcttcaa 1801 aaggtatagg aacttccgcc accatggtga gcaagggcga ggagctgttc accggggtgg 1861 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 1921 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 1981 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 2041 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 2101 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 2161 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 2221 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 2281 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 2341 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 2401 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 2461 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 2521 gcatggacga gctgtacaag ttatttctga actgctgccc gggctgctgc atggaaccgg 2581 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga 2641 agagaaaggt aatataagaa gttcctattc cgaagttcct attctctaga aagtatagga 2701 acttcagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact 2761 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 2821 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 2881 tttcacaaat aaagcatttt tttcactgcg aattctcgta aaactcttca tggcagtagt 2941 tattattctc tctctctctc tttttctttt ttcttgagac aggatatttc tctgttgccc 3001 aggctggagt gcagtggcac agtcttggct cactgcagcc tggacctcct gggctcaagc 3061 catcctccca cctcagcctc ccaagtagct ggggctacag gcacatggcc accaggccca 3121 gataattttt catttttgta gagactgagt ctcaccatgt tacccaggtt tattattctc 3181 attttttaga tgaagagact gaggtccaga gaagctcaat gacttgccta gttttacaaa 3241 tctcctgcca tcacataccc ctcagcgtcc ttaataagag ggaggccacc aactatgtgc 3301 tgggcactgt ggtggatgct ggagctatag ggttgagtat ataagaaatg gtgttgctgg 3361 agcaactgtt gcttgcttac ctgacctatc tgagaattaa ttagcagggg aacatatttt 3421 tgttttcaga ttcaatataa gaacttgtgt gggcaaaaat aaagatcagt agtaataaca 3481 gtagttccca tttgctgact gtactgtcct aagtgcatat atatatacat acacacacgc 3541 atacctatac tcctctaata ctcaaaatga tcctgtttat gtattgttaa tatgctcatt 3601 ttatttttaa atttttattt atttttattt ttatttattt ttgagacgga gtctcattct 3661 gtcgcggagg ctgaagtgca gtggtgcgat ctcagctcag tgcgacctcc gcctcccggg 3721 ttcaagtgat tctcctgcct cagcctccgg actagctggg attacaggcg cccgcctcca 3781 cgcccagcta atttttgtat ttttagtaga gatggggttt cgccatgttg gccaggctgg 3841 tctcgtactc ctgaccttga gtgatccacc tgcctcggcc tcccaaagtg ctgggattac 3901 aggcatgagc caccgcgccg ggctaatatg ctcattttag tgaggcaaaa atagaggctc 3961 agagtctgat ttgtacaaaa ctacagagca gttaagtgtc ctctcagatg tgtaccctga 4021 tctgggtgac tctaggactc taggtctcaa ctgttacaac cagttaaggg tttggggaag 4081 cactgggcca agagtcagga aaatggaagc cacaggtagt gcaaggtctt gggaatggga 4141 cgtctggtcc aaggattcac gcgatgactg gaacccgaag agccggggcc cggtttacgg 4201 ccgccatgaa gcaacgcgcg ccggtaggtt tgggaatcag ggagccctct gaataggaga 4261 ctgagttggg agggaaaggg gcttcgctgg gggagcctcg gcttcttctg ggagaaaatt 4321 cccacggcta cctagtgagc ctgcaaactg gtaggcgccg gcgtaggcgc gcgggcgggg 4381 ccgggggcgg ggcctgcggg gcgtggcggg gcgggcagag ggcggggcct gcttctcctc 4441 agcttcaggc ggctgcgacg agccctcagg cgaacctctc ggctttcccg cgcggcgccg 4501 cctcttgctg cgcctccgcc tcctcctctg ctccgccacc ggcttcctcc tcctgagcag 4561 tcagcccgcg cgccggccgg ctccgttatg gcgacccgca gccctggcgt cgtggtgagc 4621 agctcggcct gccggccctg gccggttcag gcccacgcgg caggtggcgg ccgggccctg 4681 aggcgcggga tcgatccatg acaccccccc gccccccata tctcactata attcactgtc 4741 aaatggcttt tatgatttgg agtttgggga tacttttaaa gacagggtct tggtagctcc 4801 tagatggctt gggattcact tagtaaccct tgctggccta aaattcagga acttctacct 4861 catcttccca agtactgggt tagtagggat gtccagcata tttagcttgc tttaatgatt 4921 ttgctgtact tttttctgtg tgtacaggtg catgtatatt tataaaacta acataatata 4981 ttttaccccc aaagtaccat aaccctaatt aatgctatta aatgttttgt atgctgggct 5041 ctgggcatac taatataacc aatattatac aatgcaaaac aattttggaa tatcttctgt 5101 aaaattatct tattagtaag ttgtaaccat ctcagacttc taagtttgag tttgtaaagg 5161 atcagttttt tttggtgacc tacacacaca cacacacaca cacacacaca cacacacaca 5221 ggggggtgga ttctgagaat ttagagaatt tagatggtta ataccagttt ttatttttat 5281 tctgtgtgat atttatttat taagatttat ttatttatta catgtaggta cactgtatct 5341 gtcttcagac acaccagaag agggcatcag atctcgttac agatggttgt gagtcaccat 5401 gtggttgctg ggatttgaac tcaggacctc tggaagaaca gtcagtgctc ttaaccgttg 5461 agccatctca ccagccccgt gatattttat ttaacttact agaatatact gtgttaaaac 5521 atattttagg ggattgggga gatggcccat tctgtaaagt gtctgccatg tcatcttcag 5581 gacctgcgtt tgtaacccca gcatggagaa gacagagaca gggagacccc tgggcttcct 5641 ggcaagctag actagcaaat ttggttaact aggttcaatg agagactcag tctcaaaaca 5701 taagaagtga attttttaga cccaatttca atctctgaac tccatacgta tgcacatgta 5761 cctgcatata aatatatata cacacccata aacacatgca cacacaaaaa atggttcaaa 5821 agttcttaaa atgtataaga gtagaaagtg tagagccggg agtggtggcg cacgccttta 5881 atcccagcac ttgggagcag aggcaggtgg atttctgagt tcgaggccag cctggtctac 5941 agagtgagtt ccaggacagc cagagctata cagagaaatc ctgcctcaaa aaaccaaaaa 6001 taaagtgtat atactaatct ttcatgccta aaatatttat ttttgccaca tttttcatac 6061 ctctttaata tttttctttt tctctttgtg cttcaaagga aattttgtgt catgttttac 6121 ttttcattct tgactctatt cagcagtaag acgcagcatg gctttcttag agaagtataa 6181 tctattacaa aacggtaaca tttaactgct ctacataccc tcttgtagct aaattcctct 6241 gttaaactaa gacttattat ctaaacgtaa ttaaatgttg atatcttata atgctccact 6301 ttgaaacagc tgtctttctt ttgcagatta gcgatgatga accaggttat gacctagatt 6361 tgttttgtat acctaatcat tatgccgagg atttggaaaa agtgtttatt cctcatggac 6421 tgattatgga caggttagta agacctcgat tgaagttatt tttagagggt gtgtgtgtgg 6481 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta agagagagag gagaggcatg 6541 tgtgatatat atgtgaagtg tattgcatgt acatggaatc cagagaagga cattaggtgt 6601 cctactctgt tgctctctta ttcccttgaa tcagtgtctc ttgctgaacc tggggcatac 6661 atactgattt ggctaggctg cctggctagc aagacccagg gatctgcttg tctctgcccc 6721 ccagtgctga cgttacaagc aggtgcaacc acatctggtt tttatatggg tactggggga 6781 tctgaactta gggcctgatg cgtgcccaag tgctctcacc cgccaagcca cctctccagc 6841 caaagggttt tttttcttgt attaattcta acttagcaac tgttgagtag ttgatgtttg 6901 aatataagct aagccaactt attagaataa atgttttcta ttttttactt gtatgtgtga 6961 gtgcctgcat gtatgtatgt gaagcatatg tattccagga tgtcagaatt gtgatggatt 7021 ccctggaact ggagttacag accattgtga accactgtgt atgggtgctg ggaactgaac 7081 tttaggtcct ctgcaaaaac agtaaggatc ttaactgata aactatcttt ctagccctaa 7141 ggctttctac ttttaaagtt cgtttctctt tctctctctc tctctctctc tctctctctc 7201 tctccctctc cctccctcct cctcctcctc ctctttcccc cctctcgcct cctctttctc 7261 tctcccctcc ctctctctct ctcacacaca cacacagaca gagagaggag agagaaagga 7321 agccttacta attaattacc tagttttaga cataactagt gggaatccat agtaagtaaa 7381 tcaacattct tgaagagagg ttagaagaat tggctctcgt tgtccatcga ggctgtataa 7441 gattattctt ctgttttctc agcttaccat acatattttc tacgtagaca ataccaattc 7501 aaatacactg tacgtaaagt atatgcgcac acacacacac acacacacac acacacacac 7561 acaagttaca tattgtcata tagtactaaa atttatggac tttttcatat taatacatct 7621 agagctatca tgttttttat cttttaaaaa ttactttacg gggctacaga gatgtctcag 7681 cagttaggag cactgactgc tcttccagag gtcctgagtt caattcccag caaccacatg 7741 gtggctcaca accatctgta atgggatctg atccactctt atggtgtgtc tgaagacagt 7801 gacagtgtac tcatatacat gaaataaatc tttaaaaaat tactttacca tcagggtata 7861 tatatatata tatatgtgtg tgtgtgtgtg tgtgtgtatg aagtatttgc tatttgagag 7921 ggtctcactg tttagctcag tctggcttgg aacgttctgt atagcccaga ctggcctcaa 7981 ctcagcagtc cccctgaaag gctctcatat gtggagatta caggtatgtg ctactgtgcc 8041 cagccctaca tgttactttg tttttgaaat agggtatcaa atagcttaga ctggcttcta 8101 agtcagtgtg tagctaagga tggtctgagc ttctgaacct gctacttcaa ctcctggtgt 8161 gccatcatgg ccagtttgca cagttgtgag agtagaactc aggacctcct atgtgttact 8221 agacatgttc tctaccagct gagctacatc ccaattcgcc tacatttact tttatttttt 8281 aaaagattta tttatgtata cagcactctg cctgcatgta catctgcatg ccagaagagg 8341 gcaccagatc tcatcataca tagttgtgag ccaccatgtg gttgctgggg attgaactca 8401 gggcctctgg aagagcagtc agtgctctta acctctgagc catctctgta gccccctaca 8461 tttattttaa agattatagg ctattctatt catagatgtg ccataactga tttaaccaga 8521 tcttcattat tttgtactta gtagagtata ttagagggaa tgaatcttaa atacacattt 8581 tgacacttgc tcagctttag acaagtaaaa atcactggtc aaggaaatgg tgcttagtgg 8641 atgcttctga actgtcaaat tgtcctccaa caagttacat cattttatac ttccaacgtt 8701 atgtgagagt tcatttttct ttaggctctt ctagtatttt atatttgaga aataaaattt 8761 aaccaaaatt tgccaatcta gtaagcttgt ttttatttgt atctttaaat tttatgatag 8821 atgttatagt gtactctcct ctcccctccc ctcccctctc ctcctcagca ggatcttgtt 8881 atgtagccct aagggatcta atacttactc tgtagtccag gctgtccttc agctcatgag 8941 acacaccatc atgcttggca tgtttcttgt tctgaaaggt atctggtttt aattttggtt 9001 gagctggtgt gttggtgtat atctgtaatc tcagcacagg caagtagaga caaggattat 9061 gattacagtc cagcctgggc tatttagagg gattctcttt aatctttcct catgccccaa 9121 aatcttacct ttggtatatg aaaaatagtc tccacttctg caaaatattg ctttatgaag 9181 taagaattaa ttcccttcat agagacaagg aatgtgtcct gtaaaagttt aatgtgtaag 9241 aagtatttgt tataaaagat aaatattcag aatcttcttt ttaattcctg attttatttc 9301 tataggactg aaagacttgc tcgagatgtc atgaaggaga tgggaggcca tcacattgtg 9361 gccctctgtg tgctcaaggg gggctataag ttctttgctg acctgctgga ttacattaaa 9421 gcactgaata gaaatagtga tagatccatt cctatgactg tagattttat cagactgaag 9481 agctactgtg taagtataat taacttataa ttaaaaaaat agggccattc tagttttatc 9541 tatatttttt ttaaacttgt gcaaactatg ctacataatc aacttacttg attatgtaat 9601 atatatagta atagttggaa ttactattat tacatattta tttttctgtg ctggggtaga 9661 acccagggaa ctaagtactg taataaccga gcttcattca tcctagtcat ttttaatttt 9721 tttttttttt tttgtttttt cgagacaagg tttctctgtg tagccctggc tgtcctggaa 9781 ctcactctgt agaccaggct ggcctcgaac tcagaaatct gcctgcctct gcctcccagt 9841 gctgagatta atgacgtgca ccaccactgc ctggcatttt tttaaagttt taatttgaga 9901 ccctttctca ctaacttgct cacactatcc tcgaactcat tacgtgggct ggtctagaaa 9961 tttcagtcaa cttgcctcag cttctcaagt agctgggatt acaggcaggt gctaccactt 10021 ctggccatga tcattgttgt tgttgctatt attattatta ttatttcact gatacttcat 10081 atcacaacac agaatagtaa ctgtagttca tgaccaacaa aaaagttgta cagtttctct 10141 gttgtcttat tttgatctgg attttgattt gtttcatggc actgggcagg ctaatctcaa 10201 actgcatggc tcaaatagtc cttctgtctt aatctcccat gttctggaag tataggtatc 10261 actacaccta gctgtatttt catattttaa aaaaaacgcc taagaattgc tattgaatga 10321 ggcggagaga tggctcagca gtcacaagta cttgtagagg actggggttt ggttactagc 10381 acctcaaaga tggcttacaa ttatctgtag ctccaattcc agaggatctg tgtggtaaag 10441 ctgcatgtat atgggcatgc aggtaaaact gctaaacata taaaaatagc tcagtcttaa 10501 gagaattggt cgactttttc gccctttgac gttggagtcc acgttcttta atagtggact 10561 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 10621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 10681 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 10741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 10801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 10861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 10921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 10981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 11041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 11101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 11161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 11221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 11281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 11341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 11401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 11461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 11521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 11581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 11641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 11701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 11761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 11821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 11881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accaggtcga 11941 gtagggataa cagggtaatg gatcctcgtt gggcacctgc actcatgtac gcaggcccac 12001 atatagatgt acacacgcat atcattcaaa atagaaatta tttatacaag tagtgcgttg 12061 gagagctggc tcaggagttg ttaatgcttg ctacccttgc aaaggaccca agttcaattt 12121 ccagcacctg tgttgggcat ctcacaacca catgtaaatc tagtttcagg ggatttcacc 12181 cctcttctga actcttcagg cacttatacc cacaaatgga catctccaca tagagacaca 12241 tacttttaaa aaagtaatct agggtctaga gaaatggtta tagaccacgg actgggatca 12301 gttcccagaa gccacatcct gtggctcaca attgcctgtg aatcaagttc tagatctggt 12361 ggcctcgtct agcctgcagt catcaacaaa cacatgcaca catccataca cacataattt 12421 aaaagaaagc gaaagtaacc tataatgcta aacaaagcac attctcagtg tagactgcag 12481 tggtgatgaa ccttgaaaat atattaatag aagccagaca cgtaagtcac aaatggtata 12541 actcaattta tatgaaattc ccagagagtc caaagttaaa aaaaacaaaa aggcagatta 12601 ttggttgtca gtagctacag agatgggaca ttgaagatat taataggtat agggttgagg 12661 attcatggcc tttccctctt ccatgttagc atgtctattg ttgttaacct tattcaactc 12721 atgttagggc aatcatattg atgagactta atgagtatag cttgacattc ctagaagaca 12781 gagtctcaca tcagactctc tgatcctctg actcttagaa tctttcaaca ccctcttctg 12841 aaatgatctc tgagcctttg gttgtgactg aattatgaga aagaatttta tgttgaatga 12901 attttacttc agcattttta caaaactgaa gctggggatg gcgtttcatg cgtgcagtcc 12961 ctaccatttg cctataagcc tggaggaggc agaagtagaa ttaggctttt cagggacatc 13021 ttcagctgca tcatgagctt gaggacaact tgagctacat aagattccca tccccacccc 13081 cacacacaca cacacatcta gtatgtacct agtatgcagt cttttcatat gtttaaaatg 13141 tagaaaggta tatgtgaagt ctcccttttc cccacaactt agtccctcca aagtattgca 13201 tcagtttcct tgtacatgat tttatgcata tataagatac actatttctc tctctctctc 13261 tctccttccc tccttccctc caacctctgg gtatgagttg agggttatgt atgtttcaag 13321 ttgtaataag aattgccact caaacatttc atcccatttt aagttaagtt tgtctgattt 13381 tccactttag aagggcagcc atgatggctc atgcctataa tcccatccta gcacttgtaa 13441 gcctaataca taagactgga ggattgtcat cagcttgagg ctagcctggt ctacctaatg 13501 agttccagcc agccaagagt agaatataag accctgtctc caaaaaatgt accaaagggg 13561 ctggaggggt aactcagtgg ttaagaacag tagctgctct tccagaggac ctgggttcaa 13621 ttcccagcac ccacatggca gcttgcaact atctgtaact gtccagcttt gggatatccg 13681 acaccattat accaattaca taaaatgaaa aaattgtaaa atgtgctaaa atgagtgggg 13741 agggggagca tggtgtggtt ctgtgggctt gggattttgc catgaatcca tgactatatc 13801 tcagcaatat gtggattatt tctggaaacc tgtggggcat gggtgtatgg gaagagggga 13861 gagagagaga gcccgcatcc agccagagtt cctgtgctct gggcaggcgg acgtgggagg 13921 actgctggac gatttccact tggccctggg tgggcatctg gctgtgtgaa gccactgacc 13981 ccactcagcg gggggtagat aaggggcatt tcccatacca ggttctcaga ctccagcatt 14041 ccacgctgga tacagcagaa gagctgagaa cagaataggg gccccaggga gacactctcg 14101 tcccagagat aaagaaggca gagggagaga gattcctacg caggcgagag gccttagtct 14161 gggccttgat gaacagagac ggtctatggt tttagagctt tattgtagaa aggcaggggg 14221 aaagagagaa ggtagaaaga gagagaccag ccatggccaa gaggagagaa ggggggaaag 14281 gagagagaga agaaaggcta gagagtaaga aaggttaaag agacagaagt aaaagcttag 14341 agtaagagag agagagagag caagagggag agtaagaaaa gtaaaagctt aagagagcga 14401 ggaggggcca gcccctctaa tagtgggctt gttatcttgt tgttgctagg taactgtgaa 14461 gcgtctagcc tgaaggccag aagcttggga cattgtctat gtgactgaaa gccatgagcc 14521 tctactgtgg gggctgtgga ggtggtaact tggacaggag ccaggggttc caggagacat 14581 gagggaatgc cttctgtccc atgtaggtag gaattaccac caccggcttt caccgtagtt 14641 cactggagac tgtttgtaca tagcccattg ccccacagaa accaggcact cactgtttca 14701 tttgcataag aattagaagg ctttattttg gctgggattg gtgacttgcc ccagaaattc 14761 cagcttttgg gaagccgaat cctggagaac caggagttca aggtcatcct caggtacctc 14821 atttgttcaa gaccagcctg tgctaccatg agaccttgtc cctactcccc tcaaccccaa 14881 aacacgctgt tttcatttta taggttgtac taaccatgga ggatggtcat ttgcctttct 14941 cttgatttca gaagcgtagc gcgggctttg caccatgatc tagtgtttcc agataggctt 15001 gcctctccac agccaggctt atcctgcttc agtcccatgt ttggcaaggg caacatatga 15061 ctgaagaact tgcatagcct tatggtgtat catgactctt agccccagtt tataactgtc 15121 taacagctgg tggcttttgt gaaaaaggaa agcatcattt gaacttttaa cttttcagct 15181 gtttgttttg actttgcata aatactgaga ctagctagga aatgagcttt tgtttcattt 15241 gctaaaaatg attttttttt catgaggcaa tttcaagcaa tttgaattta gcaatctcta 15301 cttgtctgaa aagaagttat taattaaaaa aaaacaaaca aacaggaacc tattatgctg 15361 gctagtcaca tgaaagaatc caaatgaaca cagtcaaggc tgaatcaaca catgaaatta 15421 ttttctaatt ccccaaatta catttatgct aattagtatg tagtaaaaca ttttaattgt 15481 ttccaatgtt aaacattaca gaaataattt tgatgttttc ctaataaccc agcctttgat 15541 catgtttgca tttatttgat tgttgtcagt ttattaaata attaagtaag agcaacaaga 15601 taaaatgact tcatgagagt ccttttctgt aacttagaac gtcagtagtc ataggaactg 15661 cggtcgttcc cttacctttc agaagtaggc taaagagttg aacgcaaagg tggtttttcg 15721 aatcttcctg atccttaagc ccccactagg tttatattct atatttgata tcggattcga 15781 acccctcgag cttaggatcc ggaaccctta atataactaa gttattaggt ccctcgaaga 15841 ggttcactag tactggccat tgcggccgct tcgaggctgc ctcgcgcgtt tcggtgatga 15901 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 15961 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 16021 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 16081 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 16141 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 16201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 16261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 16321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 16381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 16441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 16501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 16561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 16621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 16681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 16741 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 16801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 16861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 16921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 16981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 17041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 17101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 17161 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 17221 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 17281 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 17341 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 17401 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 17461 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 17521 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 17581 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 17641 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 17701 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 17761 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 17821 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 17881 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 17941 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 18001 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 18061 gttccgcgca catttccccg aaaagtgcca cctgacgg //