LOCUS       Exported                 787 bp ds-DNA     linear   SYN 02-JUL-2021
DEFINITION  Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    PX458_HHEX_2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 787)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     131..150
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        230..305
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        402..687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
       61 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      121 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      181 atatatcttg tggaaaggac gaaacaccgt ctgaacatgc caatgccagg ttttagagct
      241 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      301 ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt ccgtttttag
      361 cgcgtgcgcc aattctgcag acaaatggct ctagaggtac ccgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat agtaacgcca
      481 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      541 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      601 cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      661 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      721 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      781 gcagcga
//
