LOCUS Exported 15102 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Cre for circuit mapping and genetic manipulation. ACCESSION . VERSION . KEYWORDS RabV CVS-N2c(deltaG)-Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15102) AUTHORS Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A TITLE Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. JOURNAL Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. PUBMED 26804990 REFERENCE 2 (bases 1 to 15102) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15102 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 3325..4356 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" CDS 6531..6542 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS complement(8334..8345) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(11542..11560) /label=T7 Term /note="T7 terminator, reverse primer" terminator 11556..11603 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(11631..11647) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11631..11647) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11644..11666) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11655..11671 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11679..11709) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11724..11745 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(11862..11879) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12033..12621) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12113..12132) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(12792..13652) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 13415..13434 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(13653..13757) /gene="bla" /label=AmpR promoter rep_origin 13784..14239 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(13871..13890) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 14081..14102 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 14365..14387 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 14379..14396 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 14380..14396 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 14466..14769 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 14770..14973 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 14924..14944 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 15018..15037 /label=T7 /note="T7 promoter, forward primer" promoter 15018..15036 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa 61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt 121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa 181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt 241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc 301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga 421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca 481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa 541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc 601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg 661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc 781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat 841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca 901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg 1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga 1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag 1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc 1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg 1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg 1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca 1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact 1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa 1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta 1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc 1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga 1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca 2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc 2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga 2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt 2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc 2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag 2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga 2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg 2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga 2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat 2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga 2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg 2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg 2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg 2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct 3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc 3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg 3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag 3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc 3301 tcaaaagacc ccgggaaagc caccatgtcc aatttactga ccgtacacca aaatttgcct 3361 gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg 3421 gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg 3481 gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc 3541 gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg 3601 ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct 3661 gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa 3721 caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc 3781 gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta 3841 cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga 3901 atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca 3961 cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat 4021 gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc 4081 accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt 4141 tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt 4201 gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct 4261 ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca 4321 ggggcaatgg tgcgcctgct ggaagatggc gattaagcta gcggcgccta gccggtcatc 4381 cttttgacga ttccagtccc gaggataacc tcctctcggg attgggggga atctttggat 4441 ccagtagtcc tccttgaact ccgtccaaca gggcagattc aagagtcata agactttcat 4501 taatcatttc agttgatcag acatggtcgt gtagattctc ttaatacggg agatcttcta 4561 gcagtttcag tgaccaacgg tgctttcatt ctccaggaac tgataccaaa ggttgtggac 4621 aggccaaggg gtacttcgga tgactctgtg cttgggcaca gaaagaggtc gtagtgtgcc 4681 ccccgatagc ggactcgaca tgaatcaact aagaaaggca atctgcctcc catgatggac 4741 ataagcaata gttcacaacc atcttgcatc tcagtgaagt gtacataact attgagggct 4801 gggtcatcta agcatttcag tcgagaaaaa aactgtagac caaaagaaca actagcaaca 4861 cttctcatcc agagacccat atcaagatgc tagatccggg agaggtttat gatgacccta 4921 ttgatccaat tgagtcagag gctgaaccca gaggaacccc cactgtcccc aacatcttga 4981 ggaactccga ctacaatctc aattctcctt tgatagagga tcctgccaaa ctaatgttag 5041 aatggttgaa gacagggaac agaccttatc ggatgacttt gacagacaat tgctccaggt 5101 cttacaaagt tttgaaagat tatttcaaga aagtagattt gggttctctc aaagtgggcg 5161 gaactgctgc acagtcaatg gtttctctct ggttgtgtgg tgcccactct gaatcaaaca 5221 ggagccggag atgtataacc gacttggccc atttctattc caagtcatcc cccatagaga 5281 agctattgaa ttgtacgcta ggaaacagag gcctgagaat cccaccagag ggggtgttaa 5341 attgcctcga gagggtcaat tatgacaagg catttgggag gtatctggcc aacacgtatt 5401 cctcttactt gtttttccat gtaatcacct tatacatgaa tgccttagac tgggaagagg 5461 aaaagaccat cctggcatta tggaaagata taacctcagt ggataccgag aaggacttgg 5521 tcaaattcaa agatcaaata tggggactgt tgattgtgac aaaggacttt gtttactctc 5581 agagttcaaa ctgtcttttt gacagaaact acacactgat gctaaaggat cttttcttgt 5641 ctcgattcaa ctccttaatg attctgcttt ctccccctga gccccgatac tcagatgact 5701 taatatctca gctgtgccag ctatacatcg ctggggatca agtcttgtcc atgtgtggga 5761 actccggcta tgaagtcatc aaaatattgg agccatatgt cgtgaacagt ttggtccaga 5821 gggcagagaa gtttaggcct ctcatccacc ccttgggaga ctttcctatg ttcataaaag 5881 acaaggtgaa tcaacttgaa gggacttttg gtcccagtgc aaagaggttt tttagggttt 5941 tagatcaatt cgacaacata catgacttag tatttgtgta tggctgttac agacattggg 6001 ggcaccccta tatagattat cggaagggtc tgtcgaaact atatgatcaa gttcacatta 6061 agaaagtaat agataagtcc taccaggagt gtttagcaag tgacttggcc agaaggatcc 6121 tcagatgggg atttgacaag tactccaaat ggtatctaga ttcgagattc cttgcccgag 6181 accacccctt gactccttat gtcaagaccc aaacatggcc acccaaacat atagtagact 6241 tggtggggga cacatggcat aagctcccga tcacgcagat ctttgaaatt cctgaatcaa 6301 tggacccgtc agagatactg gatgacaaat cacattcttt caccagaaca aggttagctt 6361 cttggctgtc agagaaccga ggggggcctg ttcctagcga gaaggtcatt atcacggccc 6421 tgtctaagcc acctgtcaat ccccgagagt ttttgaaatc tatagacctc ggaggattgc 6481 cagatgagga tttgataatt ggcctcaaac caaaggaacg ggagttgaag atcgagggcc 6541 gattctttgc tctaatgtca tggaatctaa gattatattt tgtcatcacc gaaaagctct 6601 tggccaacta cattttgcca ctttttgacg cactgactat gacagacaac ctgaacaagg 6661 tgttcaaaaa gttgatcgac agggtcaccg ggcaagggct tttggactat tctagggtca 6721 catacgcatt tcacctggac tatgagaaat ggaacaatca tcaaagattg gagtcaacag 6781 aggatgtatt ctctgtccta gatcaggtgt ttggattgaa gagggtgttt tctagaacac 6841 acgagttttt tcagaagtcc tggatctatt attcagacag atcagacctc attgggttat 6901 gggaggacca aatatattgc ttggatatgt ctaacggccc aacctgctgg aatggccaag 6961 atggcgggct agagggcttg cggcagaagg gctggagtct agtcagttta ttaatgatag 7021 atagagaatc tcaaaccagg aacacaagaa ccaagatact agctcaagga gacaaccagg 7081 ttctgtgtcc tacatacatg ttgtcaccgg gattgtctca agaggggctt ctctatgagt 7141 tagagagcat atcaaggaat gcactctcaa tataccgagc tatcgaggaa ggagcatcta 7201 agctggggct gatcatcaag aaggaagaga ccatgtgtag ttatgacttt ctcatatatg 7261 ggaagacccc cttatttcga ggcaacatat tggtgcctga atccaaaaga tgggcccgag 7321 tctcttgcat ctctaacgac caaatagtca acctcgccaa tataatgtcg acagtatcca 7381 ccaatgcgct gacagtggca caacactctc aatctctgat caaacctatg agggattttc 7441 tgctcatgtc agtacaggca gttttccact acctgttgtt tagcccaatc ctaaaaggca 7501 gagtttataa gattctgagt gctgaagggg agagctttct cctagccatg tcgcggataa 7561 tctacctaga tccttctttg ggaggggtgt ctggaatgtc tctcgggagg ttccatatac 7621 gtcagttctc agaccctgtc tctgaagggt tgtcattctg gagagagatc tggttaggct 7681 ctcatgagtc ctggattcac gcgttgtgtc aggaggccgg gaaccccgat cttggagaga 7741 gaacactcga gagcttcact cgccttttag aagatcctac taccttaaat atcaaaggag 7801 gggccagccc taccattcta ctcaaggatg ctatcagaaa ggctctgtac gacgaggtgg 7861 acaaggtgga aaattcagag tttcgagagg caatcctgct gtccaagacc catagagata 7921 actttatact ctttttaaaa tctgttgagc ctctgttccc tcgatttctc agtgagctct 7981 tcagttcgtc tttcttggga ataccggagt caatcattgg actgatacaa aactcccgga 8041 caataagaag gcagtttaga aagagtctct caagaacttt agaagagtcc ttctacaact 8101 cagagatcca cgggattaat cggataaccc agacacctca aagggtcgga agggtgtggc 8161 cttgctcttc agagagggcg gatctactta gggagatctc ttgggggagg aaagtggtag 8221 gcacgacagt tcctcaccct tccgagatgt tggagttgtt tcccaaatcc tccatttcct 8281 gcacttgtgg agcaacaggg ggaggcaatc ctagagtctc tgtatcagta ctcccgtcct 8341 tcgatcagtc atttttctca cggggccccc taaagggata cttgggctcg tccacctcta 8401 tgtcaaccca gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaaaaggg 8461 ctctatcgtt aaaagaatct ataaactggt tcatcaatag gaattccaat ttggctcaaa 8521 ctctaattgg aaacatcatg tctctgacag gccctgactt ccctctagaa gaggcccctg 8581 ttttcaaacg gacagggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag 8641 ggtattcttc tgtttgccct aaccttctct cccatatctc tgttagtaca gacactatgt 8701 ctgatttgac ccaaaacggg aagaactatg atttcatgtt tcagccattg atgctttatg 8761 cgcaaacatg gacatcggaa ctggtacaga gggatacaag acttagagac tccacgtttc 8821 actggcacct tcggtgcaac agatgtgtga ggcccattga tgatataaca ctggaaactt 8881 ctcagatctt cgagttcccg gatgtgtcaa aaaggatatc caggatggtt tctggagctg 8941 tccctcaatt tcagaagctt cctgatatcc gtctaagacc aggtgatttt gaatccctaa 9001 gtggtagaga aaagtctcgc catatagggt cagctcaggg gctcttatac tcaatcttag 9061 tagcaattca cgactcagga tacaatgatg ggaccatctt ccctgtcaac atatacggca 9121 aagtttcccc cagagactat ttgagagggc ttgcaagagg gatcttgata gggtcctcga 9181 tttgcttctt gacacgaatg acaaatatta acattaacag acctcttgaa ttgatctcag 9241 gggtaatttc ctatattctc ctgaggctgg ataatcatcc ctctctgtat ataatgctca 9301 gagaaccgtc tcttagagga gaaatattct ctatccctca gaaaatcccc gccgcttacc 9361 caaccactat gagagaaggc aacagatcga ttttgtgtta cctccaacac gtgctacgct 9421 atgagcgaga ggcaatcacg gcgtccccgg agaatgactg gctgtggatc ttctcagact 9481 tcagaagtgt gaaaatgacg tacttgaccc tcattactta ccagtctcac ctcctactcc 9541 agagggttga gagaaacttg tctaagagta tgagagctac tctgcgacaa atgggttcct 9601 taatgaggca agtgctgggt gggcacggag aagatacctt ggagtcagac gatgacattc 9661 aacgattact aaaagactct ttgcgaaaga caaggtgggt ggatcaagag gtgcgccatg 9721 cagctagaac catgaatgga gattacagcc ccgacaagaa agtatcccgt aaggtaggat 9781 gttcagaatg ggtctgctct gctcaacaga ttgccgtctc cacctcagcc aacccggccc 9841 ctgtctcaga gcttgacatt agggccctct ctaagcggtt tcaaaacccc ttgatctcgg 9901 gcctgagagt ggttcagtgg gcaaccggtg cccattataa gcttaagcct attctagatg 9961 atctaaatgt tttcccatct ctctgtcttg taattggaga cgggtcaggg ggaatatcaa 10021 gggcagttct caacatgttt ccagattcta agcttgtgtt caacagccta ttggaggtga 10081 atgatctgat ggcttccgga acacatccac tgcctccttc agcaatcatg agtggaggag 10141 atgacatcat ctccagagtg atagactttg actcaatatg ggagaaaccg tccgacctga 10201 ggaactcggc cacctggaga tacttccagt cggttcaaaa acaggtcaac atgtcgtatg 10261 acctcattat ttgtgatgca gaagttactg atattgcatc tatcaaccgg ataactctgt 10321 tgatgtctga tttcgcattg tctatagatg gaccacttta tctggtcttc aaaacttacg 10381 ggactatgct agtaaacccg gactataaag ctattcaaca tctgtcaaga gcgttccctt 10441 cggtcacagg gtttgtaacc caagtaactt catccttttc ttctgagcta tacctccggt 10501 tctctaaacg aggaaagttt ttcagggacg ccgagtactt gacctcttcc acccttcgag 10561 agatgagcct tgtgttgttc aattgcagca gccccaaaag tgagatgcag agagctcgtt 10621 ccttaaacta tcaagacctg gtaaggggat ttcctgaaga gatcatatca aatccttaca 10681 acgaaatgat cataactctg attgacaatg atgtagagtc cttcctagtc cacaagatgg 10741 tggatgatct tgagctacag aggggaactc tgtctaaagt ggctatcatt atatccatca 10801 tgatcgtttt ttccaataga gtcttcaaca tttccaaacc tttgactgac cccttgttct 10861 atccaccgtc tgatcctaaa atcctgaggc acttcaacat atgttgcagt actatgatgt 10921 atctatctac cgctttaggc gacgtcccta acttcgcaag acttcatgac ctgtataaca 10981 gacctataac ttgttacttc agaaaacaag ttattcgagg gaatatttat ctatcttgga 11041 gttggtccga tgatacccca gtgttcaaga gagtagcctg taattctagc ttgagtctgt 11101 catctcactg gatcaggttg atctacaaga tagtgaagac taccagactc attggcagca 11161 taaaagacct atcaggagag gtagaacgac atcttcatgg gtataacaga tggatcaccc 11221 tcgaggatat ccgatctaga tcatccctac tagattacag ttgtttgtaa gccggatatt 11281 accgaaagcc tgtgcatgct aaaattcttg tatgatgcat cttgaaaaaa acaagatctt 11341 gaatccggac ctctggttgt ttgattgttt tttccatctt tattgttttt ttgttaagcg 11401 tgggtcggca tggcatctcc acctcctcgc ggtccgacct gggcatccga aggaggacgc 11461 acgtccactc ggatggctaa gggaggggcg gggatccggc tgctaacaaa gcccgaaagg 11521 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta 11581 aacgggtctt gaggggtttt ttgctgaaag tcgcgcttgg cgtaatcatg gtcatagctg 11641 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 11701 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 11761 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 11821 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 11881 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 11941 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 12001 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 12061 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 12121 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 12181 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 12241 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 12301 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 12361 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 12421 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 12481 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 12541 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 12601 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 12661 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 12721 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 12781 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 12841 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 12901 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 12961 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 13021 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 13081 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 13141 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 13201 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 13261 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 13321 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 13381 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 13441 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 13501 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 13561 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 13621 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 13681 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 13741 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc 13801 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 13861 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 13921 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 13981 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 14041 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 14101 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 14161 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 14221 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat 14281 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 14341 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc 14401 gcgcctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 14461 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 14521 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 14581 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 14641 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 14701 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 14761 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 14821 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 14881 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 14941 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 15001 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagattaag 15061 cgtctgatga gtccgtgagg acgaaacccg gcgtaccggg tc //