LOCUS Exported 4992 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pDelila (GB0079) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4992) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 4992) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4992 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(2042..2060) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2043..2060) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2078..2094) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2078..2094) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2091..2113) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2102..2118 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2126..2156) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2171..2192 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2309..2326) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2480..3068) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2560..2579) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3239..4099) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3862..3881 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4100..4204) /gene="bla" /label=AmpR promoter rep_origin complement(4282..4737) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4419..4440) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4631..4650 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4863..4885 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4877..4894 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4878..4894 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 4901..4920 /label=T7 /note="T7 promoter, forward primer" promoter 4901..4919 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 4950..4959 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 4954..4963 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgaagctc aagacttcat agtaactttc tgaagagctt gtttgatcac acttgctgat 61 gcaaccttca atcccttgca cttggctttt atggtaatgg atataattcc atctctgttg 121 gaagattgaa cagtttcgga atccaaactt agtcgtctta cggcttccat cacttcaagc 181 aatacaaact ccttggaaga acaactcacg acaatcaaca catccttgtt tgtaatgttc 241 acagttatat tatctgttaa ggaatctttc aatcgacctc tgctatttac ggctccaatc 301 ttgtccgtat cagaagcctt tctcttgttt gtcaacggtt tcttgacatt acttgtcctt 361 gttgtgccat aattatcgga ggtcctctca atggcatcgt gcagtttagt ttttgtagtt 421 gattcccgcc cccggccctt taccattttg ttagattcca gctcgtcgac tttcctctca 481 agccctctca agtaatctat tgtatggtct agtattgata ctttgtcaac cttgccaccg 541 gatgggacta gggatgcaag aatcataaac cgttcgttta ttttctctct gcgttttctc 601 tctgacaaga cgtggtttct atcaatttca tcagccgttg gctttgcaag gcagtcactg 661 ttgccttttt gtttaccagc atcaagcctg gagttttcat gcattctagc tacttcaaaa 721 agtactttct tcagaaatct ttgtgaggtt ccgcttcggg gaacatgagt acccgacgat 781 ccatccttgt tccaactaac gaagcttgat tctctattcc catttctgaa gtagggacca 841 agaaccaact gatgggaact cttcaaaaga ttggaaagta ccccttgata atggacctcg 901 tcgccttgga cacccgtgtt ttctatctgc tgattgcatt tttgagtgct gtgcatacaa 961 ttatttgtct ccggtgcccc ttttccatca gaaagtggag caaaagactc tagattttca 1021 tgagtttgag atatacagtc actagaattc atagaactat tgagacaatt gctgattgca 1081 tcatccatga aaggccagct ttgtgtttga ggaacctccc cattgatgcc ttctgccaaa 1141 ttcgattcgt ctatgagtaa attgtctgca aagtcatcca aactgttatc aggagaacat 1201 atgttcgtgt ctggacaatt caaaagctga tcaagatcgt tttctggtat attagcatgt 1261 tcaagcgctt cacaaatgag gtcattgttg tttgtaatac tgttggagac atagttggga 1321 atcttgggaa cggtggcagg actgtccaag aatgaagttt ttatatgctg gattagattc 1381 aaatcctccg gtactagctc tgttgctccc agctcaacta ctccttctgc atatggaaag 1441 cacacaactg tctgaattga cgcactcttt gcaagcaaag aacgcgagaa aactttggtg 1501 tccgcacgat gagcgttgca tagccatact gcttgatttc gtgctaatgt tcttccaggc 1561 aacccttggc caatattgaa tatgaaagac atgcaaacca agaaaaacca ctcagcatca 1621 gtgaggtctt ctggtgataa tgcagcagta ggccttttag cttgtgtgtt ggtttcacca 1681 agtgaaagag actcataaag ttctctcaat tgatcacttc tctgcaatcc cagctgatcg 1741 ggattcaatt cgacagattg tacagttttt cgagttttaa tatctccatt gtagaaccca 1801 tcaccccact ccaagacccc tggttgtgca actgaattgg accagaaaat tgcataactc 1861 cattggatac ttctaacagc aatagcaagt tgcttcctca aattctcagg cactatcttt 1921 tggttttgga taccagtagc cattcgagac cactcatcgc ccaatcacta gtgaattcgc 1981 ggccgcctgc aggtcgacca tatgggagag ctcccacgcg ttggatgcat agcttgagta 2041 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 2101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 2161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 2221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 2281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 2341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 2401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 2461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 2521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 2581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 2641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 2701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 2761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 2821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 2881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 2941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 3181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 3241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 3301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 3361 gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc 3421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 3481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 3541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 3601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 3661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 3721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 3781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 3841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 3901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 3961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 4021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 4081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 4141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 4201 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta 4261 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 4321 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 4381 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 4441 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgctggcc 4501 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 4561 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 4621 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 4681 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca 4741 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 4801 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 4861 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg 4921 cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattgg 4981 gcgatgagtg gt //