LOCUS Exported 3193 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION GoldenBraid level 1 Alpha plant expression vector. . ACCESSION . VERSION . KEYWORDS pDGB1_alpha2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3193) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3193) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3193 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 125..144 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 338..360 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 352..369 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 353..369 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 370..426 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(439..455) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(439..455) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(452..474) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 463..479 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(487..517) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 532..553 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 632..656 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(747..1335) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(827..846) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1506..2321) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 2229..2248 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 2313..2332 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 2612..3047 /label=pSa ori /note="origin of replication from bacterial plasmid pSa" misc_feature 3174..3 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" ORIGIN 1 taacaagctt cgtctcagtc aggagagaga cccacagctt gtctgtaagc ggatgccggg 61 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 121 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 181 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 241 tgggaagggc gatcggcgca ggcctcttcg ctattacgcc agctggcgaa agggggatgt 301 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 361 acggccagtg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc 421 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 481 tccacacaac atacgagccg gaagcatata gtgtaaagcc tggggtgcct aatgagtgag 541 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 601 ccagctgggg tctctcgctt gagacgaagc ttgacaggat atattggcgg gtaaactaag 661 tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa ggccaggaac 721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 1201 atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc 1261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta acattggtct 1501 agtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta 1561 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag 1621 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata 1681 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg 1741 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca 1801 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt 1861 gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga 1921 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca 1981 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat 2041 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc 2101 cagtttagtc tgaccatctc atctgtaaca acattggcaa cgctaccttt gccatgtttc 2161 agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc 2221 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat 2281 cgcggccttg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg 2341 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa 2401 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg 2461 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc 2521 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc 2581 tggctggatg atggggcgat tcaggcgatc cccatccaac agcccgccgt cgagcgggct 2641 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc 2701 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg 2761 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg 2821 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc 2881 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat 2941 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac 3001 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac 3061 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc 3121 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg 3181 atatattgtg gtg //