LOCUS Exported 16482 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).. ACCESSION . VERSION . KEYWORDS pEMS1307 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16482) AUTHORS Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM TITLE A regulatory toolbox of MiniPromoters to drive selective expression in the brain. JOURNAL Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PUBMED 20807748 REFERENCE 2 (bases 1 to 16482) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16482 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" regulatory 202..211 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 208..924 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 208..924 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(253..274) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(514..533) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 861..882 /label=EGFP-C /note="EGFP, forward primer" CDS 937..954 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" CDS 967..987 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 991..1011 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1015..1035 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 1042..1089 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." polyA_signal 1212..1293 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1249..1268) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8938..8959 /label=F1ori-F /note="F1 origin, forward primer" promoter 9122..9226 /gene="bla" /label=AmpR promoter CDS 9227..10087 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(14294..14316) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 14402..14542 /label=bom /note="basis of mobility region from pBR322" primer_bind 14457..14476 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(14557..14574) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(14728..15316) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(14808..14827) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(15487..16347) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(16348..16452) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac gcgtggcgcg ccgaagttcc tattccgaag ttcctattct 181 tcaaaaggta taggaacttc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc 421 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 661 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 721 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 781 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac 841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 901 ctcggcatgg acgagctgta caagttattt ctgaactgct gcccgggctg ctgcatggaa 961 ccggatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa 1021 aagaagagaa aggtaatata agaagttcct attccgaagt tcctattctc tagaaagtat 1081 aggaacttca gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt 1141 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 1201 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1261 caaatttcac aaataaagca tttttttcac tgcgaattct cgtaaaactc ttcatggcag 1321 tagttattat tctctctctc tctctttttc ttttttcttg agacaggata tttctctgtt 1381 gcccaggctg gagtgcagtg gcacagtctt ggctcactgc agcctggacc tcctgggctc 1441 aagccatcct cccacctcag cctcccaagt agctggggct acaggcacat ggccaccagg 1501 cccagataat ttttcatttt tgtagagact gagtctcacc atgttaccca ggtttattat 1561 tctcattttt tagatgaaga gactgaggtc cagagaagct caatgacttg cctagtttta 1621 caaatctcct gccatcacat acccctcagc gtccttaata agagggaggc caccaactat 1681 gtgctgggca ctgtggtgga tgctggagct atagggttga gtatataaga aatggtgttg 1741 ctggagcaac tgttgcttgc ttacctgacc tatctgagaa ttaattagca ggggaacata 1801 tttttgtttt cagattcaat ataagaactt gtgtgggcaa aaataaagat cagtagtaat 1861 aacagtagtt cccatttgct gactgtactg tcctaagtgc atatatatat acatacacac 1921 acgcatacct atactcctct aatactcaaa atgatcctgt ttatgtattg ttaatatgct 1981 cattttattt ttaaattttt atttattttt atttttattt atttttgaga cggagtctca 2041 ttctgtcgcg gaggctgaag tgcagtggtg cgatctcagc tcagtgcgac ctccgcctcc 2101 cgggttcaag tgattctcct gcctcagcct ccggactagc tgggattaca ggcgcccgcc 2161 tccacgccca gctaattttt gtatttttag tagagatggg gtttcgccat gttggccagg 2221 ctggtctcgt actcctgacc ttgagtgatc cacctgcctc ggcctcccaa agtgctggga 2281 ttacaggcat gagccaccgc gccgggctaa tatgctcatt ttagtgaggc aaaaatagag 2341 gctcagagtc tgatttgtac aaaactacag agcagttaag tgtcctctca gatgtgtacc 2401 ctgatctggg tgactctagg actctaggtc tcaactgtta caaccagtta agggtttggg 2461 gaagcactgg gccaagagtc aggaaaatgg aagccacagg tagtgcaagg tcttgggaat 2521 gggacgtctg gtccaaggat tcacgcgatg actggaaccc gaagagccgg ggcccggttt 2581 acggccgcca tgaagcaacg cgcgccggta ggtttgggaa tcagggagcc ctctgaatag 2641 gagactgagt tgggagggaa aggggcttcg ctgggggagc ctcggcttct tctgggagaa 2701 aattcccacg gctacctagt gagcctgcaa actggtaggc gccggcgtag gcgcgcgggc 2761 ggggccgggg gcggggcctg cggggcgtgg cggggcgggc agagggcggg gcctgcttct 2821 cctcagcttc aggcggctgc gacgagccct caggcgaacc tctcggcttt cccgcgcggc 2881 gccgcctctt gctgcgcctc cgcctcctcc tctgctccgc caccggcttc ctcctcctga 2941 gcagtcagcc cgcgcgccgg ccggctccgt tatggcgacc cgcagccctg gcgtcgtggt 3001 gagcagctcg gcctgccggc cctggccggt tcaggcccac gcggcaggtg gcggccgggc 3061 cctgaggcgc gggatcgatc catgacaccc ccccgccccc catatctcac tataattcac 3121 tgtcaaatgg cttttatgat ttggagtttg gggatacttt taaagacagg gtcttggtag 3181 ctcctagatg gcttgggatt cacttagtaa cccttgctgg cctaaaattc aggaacttct 3241 acctcatctt cccaagtact gggttagtag ggatgtccag catatttagc ttgctttaat 3301 gattttgctg tacttttttc tgtgtgtaca ggtgcatgta tatttataaa actaacataa 3361 tatattttac ccccaaagta ccataaccct aattaatgct attaaatgtt ttgtatgctg 3421 ggctctgggc atactaatat aaccaatatt atacaatgca aaacaatttt ggaatatctt 3481 ctgtaaaatt atcttattag taagttgtaa ccatctcaga cttctaagtt tgagtttgta 3541 aaggatcagt tttttttggt gacctacaca cacacacaca cacacacaca cacacacaca 3601 cacagggggg tggattctga gaatttagag aatttagatg gttaatacca gtttttattt 3661 ttattctgtg tgatatttat ttattaagat ttatttattt attacatgta ggtacactgt 3721 atctgtcttc agacacacca gaagagggca tcagatctcg ttacagatgg ttgtgagtca 3781 ccatgtggtt gctgggattt gaactcagga cctctggaag aacagtcagt gctcttaacc 3841 gttgagccat ctcaccagcc ccgtgatatt ttatttaact tactagaata tactgtgtta 3901 aaacatattt taggggattg gggagatggc ccattctgta aagtgtctgc catgtcatct 3961 tcaggacctg cgtttgtaac cccagcatgg agaagacaga gacagggaga cccctgggct 4021 tcctggcaag ctagactagc aaatttggtt aactaggttc aatgagagac tcagtctcaa 4081 aacataagaa gtgaattttt tagacccaat ttcaatctct gaactccata cgtatgcaca 4141 tgtacctgca tataaatata tatacacacc cataaacaca tgcacacaca aaaaatggtt 4201 caaaagttct taaaatgtat aagagtagaa agtgtagagc cgggagtggt ggcgcacgcc 4261 tttaatccca gcacttggga gcagaggcag gtggatttct gagttcgagg ccagcctggt 4321 ctacagagtg agttccagga cagccagagc tatacagaga aatcctgcct caaaaaacca 4381 aaaataaagt gtatatacta atctttcatg cctaaaatat ttatttttgc cacatttttc 4441 atacctcttt aatatttttc tttttctctt tgtgcttcaa aggaaatttt gtgtcatgtt 4501 ttacttttca ttcttgactc tattcagcag taagacgcag catggctttc ttagagaagt 4561 ataatctatt acaaaacggt aacatttaac tgctctacat accctcttgt agctaaattc 4621 ctctgttaaa ctaagactta ttatctaaac gtaattaaat gttgatatct tataatgctc 4681 cactttgaaa cagctgtctt tcttttgcag attagcgatg atgaaccagg ttatgaccta 4741 gatttgtttt gtatacctaa tcattatgcc gaggatttgg aaaaagtgtt tattcctcat 4801 ggactgatta tggacaggtt agtaagacct cgattgaagt tatttttaga gggtgtgtgt 4861 gtggtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtaagagag agaggagagg 4921 catgtgtgat atatatgtga agtgtattgc atgtacatgg aatccagaga aggacattag 4981 gtgtcctact ctgttgctct cttattccct tgaatcagtg tctcttgctg aacctggggc 5041 atacatactg atttggctag gctgcctggc tagcaagacc cagggatctg cttgtctctg 5101 ccccccagtg ctgacgttac aagcaggtgc aaccacatct ggtttttata tgggtactgg 5161 gggatctgaa cttagggcct gatgcgtgcc caagtgctct cacccgccaa gccacctctc 5221 cagccaaagg gttttttttc ttgtattaat tctaacttag caactgttga gtagttgatg 5281 tttgaatata agctaagcca acttattaga ataaatgttt tctatttttt acttgtatgt 5341 gtgagtgcct gcatgtatgt atgtgaagca tatgtattcc aggatgtcag aattgtgatg 5401 gattccctgg aactggagtt acagaccatt gtgaaccact gtgtatgggt gctgggaact 5461 gaactttagg tcctctgcaa aaacagtaag gatcttaact gataaactat ctttctagcc 5521 ctaaggcttt ctacttttaa agttcgtttc tctttctctc tctctctctc tctctctctc 5581 tctctctccc tctccctccc tcctcctcct cctcctcttt cccccctctc gcctcctctt 5641 tctctctccc ctccctctct ctctctcaca cacacacaca gacagagaga ggagagagaa 5701 aggaagcctt actaattaat tacctagttt tagacataac tagtgggaat ccatagtaag 5761 taaatcaaca ttcttgaaga gaggttagaa gaattggctc tcgttgtcca tcgaggctgt 5821 ataagattat tcttctgttt tctcagctta ccatacatat tttctacgta gacaatacca 5881 attcaaatac actgtacgta aagtatatgc gcacacacac acacacacac acacacacac 5941 acacacaagt tacatattgt catatagtac taaaatttat ggactttttc atattaatac 6001 atctagagct atcatgtttt ttatctttta aaaattactt tacggggcta cagagatgtc 6061 tcagcagtta ggagcactga ctgctcttcc agaggtcctg agttcaattc ccagcaacca 6121 catggtggct cacaaccatc tgtaatggga tctgatccac tcttatggtg tgtctgaaga 6181 cagtgacagt gtactcatat acatgaaata aatctttaaa aaattacttt accatcaggg 6241 tatatatata tatatatatg tgtgtgtgtg tgtgtgtgtg tatgaagtat ttgctatttg 6301 agagggtctc actgtttagc tcagtctggc ttggaacgtt ctgtatagcc cagactggcc 6361 tcaactcagc agtccccctg aaaggctctc atatgtggag attacaggta tgtgctactg 6421 tgcccagccc tacatgttac tttgtttttg aaatagggta tcaaatagct tagactggct 6481 tctaagtcag tgtgtagcta aggatggtct gagcttctga acctgctact tcaactcctg 6541 gtgtgccatc atggccagtt tgcacagttg tgagagtaga actcaggacc tcctatgtgt 6601 tactagacat gttctctacc agctgagcta catcccaatt cgcctacatt tacttttatt 6661 ttttaaaaga tttatttatg tatacagcac tctgcctgca tgtacatctg catgccagaa 6721 gagggcacca gatctcatca tacatagttg tgagccacca tgtggttgct ggggattgaa 6781 ctcagggcct ctggaagagc agtcagtgct cttaacctct gagccatctc tgtagccccc 6841 tacatttatt ttaaagatta taggctattc tattcataga tgtgccataa ctgatttaac 6901 cagatcttca ttattttgta cttagtagag tatattagag ggaatgaatc ttaaatacac 6961 attttgacac ttgctcagct ttagacaagt aaaaatcact ggtcaaggaa atggtgctta 7021 gtggatgctt ctgaactgtc aaattgtcct ccaacaagtt acatcatttt atacttccaa 7081 cgttatgtga gagttcattt ttctttaggc tcttctagta ttttatattt gagaaataaa 7141 atttaaccaa aatttgccaa tctagtaagc ttgtttttat ttgtatcttt aaattttatg 7201 atagatgtta tagtgtactc tcctctcccc tcccctcccc tctcctcctc agcaggatct 7261 tgttatgtag ccctaaggga tctaatactt actctgtagt ccaggctgtc cttcagctca 7321 tgagacacac catcatgctt ggcatgtttc ttgttctgaa aggtatctgg ttttaatttt 7381 ggttgagctg gtgtgttggt gtatatctgt aatctcagca caggcaagta gagacaagga 7441 ttatgattac agtccagcct gggctattta gagggattct ctttaatctt tcctcatgcc 7501 ccaaaatctt acctttggta tatgaaaaat agtctccact tctgcaaaat attgctttat 7561 gaagtaagaa ttaattccct tcatagagac aaggaatgtg tcctgtaaaa gtttaatgtg 7621 taagaagtat ttgttataaa agataaatat tcagaatctt ctttttaatt cctgatttta 7681 tttctatagg actgaaagac ttgctcgaga tgtcatgaag gagatgggag gccatcacat 7741 tgtggccctc tgtgtgctca aggggggcta taagttcttt gctgacctgc tggattacat 7801 taaagcactg aatagaaata gtgatagatc cattcctatg actgtagatt ttatcagact 7861 gaagagctac tgtgtaagta taattaactt ataattaaaa aaatagggcc attctagttt 7921 tatctatatt ttttttaaac ttgtgcaaac tatgctacat aatcaactta cttgattatg 7981 taatatatat agtaatagtt ggaattacta ttattacata tttatttttc tgtgctgggg 8041 tagaacccag ggaactaagt actgtaataa ccgagcttca ttcatcctag tcatttttaa 8101 tttttttttt tttttttgtt ttttcgagac aaggtttctc tgtgtagccc tggctgtcct 8161 ggaactcact ctgtagacca ggctggcctc gaactcagaa atctgcctgc ctctgcctcc 8221 cagtgctgag attaatgacg tgcaccacca ctgcctggca tttttttaaa gttttaattt 8281 gagacccttt ctcactaact tgctcacact atcctcgaac tcattacgtg ggctggtcta 8341 gaaatttcag tcaacttgcc tcagcttctc aagtagctgg gattacaggc aggtgctacc 8401 acttctggcc atgatcattg ttgttgttgc tattattatt attattattt cactgatact 8461 tcatatcaca acacagaata gtaactgtag ttcatgacca acaaaaaagt tgtacagttt 8521 ctctgttgtc ttattttgat ctggattttg atttgtttca tggcactggg caggctaatc 8581 tcaaactgca tggctcaaat agtccttctg tcttaatctc ccatgttctg gaagtatagg 8641 tatcactaca cctagctgta ttttcatatt ttaaaaaaaa cgcctaagaa ttgctattga 8701 atgaggcgga gagatggctc agcagtcaca agtacttgta gaggactggg gtttggttac 8761 tagcacctca aagatggctt acaattatct gtagctccaa ttccagagga tctgtgtggt 8821 aaagctgcat gtatatgggc atgcaggtaa aactgctaaa catataaaaa tagctcagtc 8881 ttaagagaat tggtcgactt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 8941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 9001 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 9061 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 9121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 9181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 9241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 9301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 9361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 9421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 9481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 9541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 9601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 9661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 9721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 9781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 9841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 9901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 9961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 10021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 10081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 10141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 10201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 10261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagg 10321 tcgagtaggg ataacagggt aatggatcct cgttgggcac ctgcactcat gtacgcaggc 10381 ccacatatag atgtacacac gcatatcatt caaaatagaa attatttata caagtagtgc 10441 gttggagagc tggctcagga gttgttaatg cttgctaccc ttgcaaagga cccaagttca 10501 atttccagca cctgtgttgg gcatctcaca accacatgta aatctagttt caggggattt 10561 cacccctctt ctgaactctt caggcactta tacccacaaa tggacatctc cacatagaga 10621 cacatacttt taaaaaagta atctagggtc tagagaaatg gttatagacc acggactggg 10681 atcagttccc agaagccaca tcctgtggct cacaattgcc tgtgaatcaa gttctagatc 10741 tggtggcctc gtctagcctg cagtcatcaa caaacacatg cacacatcca tacacacata 10801 atttaaaaga aagcgaaagt aacctataat gctaaacaaa gcacattctc agtgtagact 10861 gcagtggtga tgaaccttga aaatatatta atagaagcca gacacgtaag tcacaaatgg 10921 tataactcaa tttatatgaa attcccagag agtccaaagt taaaaaaaac aaaaaggcag 10981 attattggtt gtcagtagct acagagatgg gacattgaag atattaatag gtatagggtt 11041 gaggattcat ggcctttccc tcttccatgt tagcatgtct attgttgtta accttattca 11101 actcatgtta gggcaatcat attgatgaga cttaatgagt atagcttgac attcctagaa 11161 gacagagtct cacatcagac tctctgatcc tctgactctt agaatctttc aacaccctct 11221 tctgaaatga tctctgagcc tttggttgtg actgaattat gagaaagaat tttatgttga 11281 atgaatttta cttcagcatt tttacaaaac tgaagctggg gatggcgttt catgcgtgca 11341 gtccctacca tttgcctata agcctggagg aggcagaagt agaattaggc ttttcaggga 11401 catcttcagc tgcatcatga gcttgaggac aacttgagct acataagatt cccatcccca 11461 cccccacaca cacacacaca tctagtatgt acctagtatg cagtcttttc atatgtttaa 11521 aatgtagaaa ggtatatgtg aagtctccct tttccccaca acttagtccc tccaaagtat 11581 tgcatcagtt tccttgtaca tgattttatg catatataag atacactatt tctctctctc 11641 tctctctcct tccctccttc cctccaacct ctgggtatga gttgagggtt atgtatgttt 11701 caagttgtaa taagaattgc cactcaaaca tttcatccca ttttaagtta agtttgtctg 11761 attttccact ttagaagggc agccatgatg gctcatgcct ataatcccat cctagcactt 11821 gtaagcctaa tacataagac tggaggattg tcatcagctt gaggctagcc tggtctacct 11881 aatgagttcc agccagccaa gagtagaata taagaccctg tctccaaaaa atgtaccaaa 11941 ggggctggag gggtaactca gtggttaaga acagtagctg ctcttccaga ggacctgggt 12001 tcaattccca gcacccacat ggcagcttgc aactatctgt aactgtccag ctttgggata 12061 tccgacacca ttataccaat tacataaaat gaaaaaattg taaaatgtgc taaaatgagt 12121 ggggaggggg agcatggtgt ggttctgtgg gcttgggatt ttgccatgaa tccatgacta 12181 tatctcagca atatgtggat tatttctgga aacctgtggg gcatgggtgt atgggaagag 12241 gggagagaga gagagcccgc atccagccag agttcctgtg ctctgggcag gcggacgtgg 12301 gaggactgct ggacgatttc cacttggccc tgggtgggca tctggctgtg tgaagccact 12361 gaccccactc agcggggggt agataagggg catttcccat accaggttct cagactccag 12421 cattccacgc tggatacagc agaagagctg agaacagaat aggggcccca gggagacact 12481 ctcgtcccag agataaagaa ggcagaggga gagagattcc tacgcaggcg agaggcctta 12541 gtctgggcct tgatgaacag agacggtcta tggttttaga gctttattgt agaaaggcag 12601 ggggaaagag agaaggtaga aagagagaga ccagccatgg ccaagaggag agaagggggg 12661 aaaggagaga gagaagaaag gctagagagt aagaaaggtt aaagagacag aagtaaaagc 12721 ttagagtaag agagagagag agagcaagag ggagagtaag aaaagtaaaa gcttaagaga 12781 gcgaggaggg gccagcccct ctaatagtgg gcttgttatc ttgttgttgc taggtaactg 12841 tgaagcgtct agcctgaagg ccagaagctt gggacattgt ctatgtgact gaaagccatg 12901 agcctctact gtgggggctg tggaggtggt aacttggaca ggagccaggg gttccaggag 12961 acatgaggga atgccttctg tcccatgtag gtaggaatta ccaccaccgg ctttcaccgt 13021 agttcactgg agactgtttg tacatagccc attgccccac agaaaccagg cactcactgt 13081 ttcatttgca taagaattag aaggctttat tttggctggg attggtgact tgccccagaa 13141 attccagctt ttgggaagcc gaatcctgga gaaccaggag ttcaaggtca tcctcaggta 13201 cctcatttgt tcaagaccag cctgtgctac catgagacct tgtccctact cccctcaacc 13261 ccaaaacacg ctgttttcat tttataggtt gtactaacca tggaggatgg tcatttgcct 13321 ttctcttgat ttcagaagcg tagcgcgggc tttgcaccat gatctagtgt ttccagatag 13381 gcttgcctct ccacagccag gcttatcctg cttcagtccc atgtttggca agggcaacat 13441 atgactgaag aacttgcata gccttatggt gtatcatgac tcttagcccc agtttataac 13501 tgtctaacag ctggtggctt ttgtgaaaaa ggaaagcatc atttgaactt ttaacttttc 13561 agctgtttgt tttgactttg cataaatact gagactagct aggaaatgag cttttgtttc 13621 atttgctaaa aatgattttt ttttcatgag gcaatttcaa gcaatttgaa tttagcaatc 13681 tctacttgtc tgaaaagaag ttattaatta aaaaaaaaca aacaaacagg aacctattat 13741 gctggctagt cacatgaaag aatccaaatg aacacagtca aggctgaatc aacacatgaa 13801 attattttct aattccccaa attacattta tgctaattag tatgtagtaa aacattttaa 13861 ttgtttccaa tgttaaacat tacagaaata attttgatgt tttcctaata acccagcctt 13921 tgatcatgtt tgcatttatt tgattgttgt cagtttatta aataattaag taagagcaac 13981 aagataaaat gacttcatga gagtcctttt ctgtaactta gaacgtcagt agtcatagga 14041 actgcggtcg ttcccttacc tttcagaagt aggctaaaga gttgaacgca aaggtggttt 14101 ttcgaatctt cctgatcctt aagcccccac taggtttata ttctatattt gatatcggat 14161 tcgaacccct cgagcttagg atccggaacc cttaatataa ctaagttatt aggtccctcg 14221 aagaggttca ctagtactgg ccattgcggc cgcttcgagg ctgcctcgcg cgtttcggtg 14281 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 14341 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 14401 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 14461 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 14521 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 14581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 14641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 14701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 14761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 14821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 14881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 14941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 15001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 15061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 15121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 15181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 15241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 15301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 15361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 15421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 15481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 15541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 15601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 15661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 15721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 15781 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 15841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 15901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 15961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 16021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 16081 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 16141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 16201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 16261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 16321 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 16381 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 16441 aggggttccg cgcacatttc cccgaaaagt gccacctgac gg //