LOCUS       Exported                1106 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Self-inactivating retrovirus vector containing the DsRed-Express2 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    pSIR-DsRed-Express2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(151..828)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(213..236)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(411..430)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     628..648
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
       61 cctggaccac tgatatcctg tctttaacaa attggactaa tcgcgggatc tccagcatga
      121 gatccccgcg ctggaggatc atccagccat ctactggaac aggtggtggc gggcctcggc
      181 gcgctcgtac tgctccacca cggtgtagtc ctcgttgtgg gaggtgatgt ccagcttgga
      241 gtccacgtag tagtagccgg gcagcttcac gggcttcttg gccatgtaga ttgacttgaa
      301 ctccaccagg tagtggccgc cgcccttcag cttcagcgcc ttgtggatct cgcccttcag
      361 cacgccgtcg cgggggtaca ggcgctcggt ggagggctcc cagcccagag tcttcttctg
      421 cattacgggg ccgtcggagg ggaagttcac gccgatgaac ttcacgtggt agatgaaggt
      481 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
      541 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc
      601 ggggtgcttc acgtacacct tggagccgta ctggaactgg ggggacagga tgtcccaggc
      661 gaagggcagg gggccgccct tggtcacctg cagcttggcg gtctgggtgc cctcgtaggg
      721 cttgccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
      781 caccttgaag cgcatgaagg gcttgatgac gttctcagtg ctatccatgg tggatcagat
      841 cgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
      901 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
      961 ctcgacgcgc tttgtgaggc ctctggcctg aggcagcgcc tttatacgac agttggcgga
     1021 ccgaactgag aacctgaaag aagtcggcgg gaagtcccgc cccggtgggg gaggggaaat
     1081 ctaaagggcc aaaccgaaat aggggg
//
