LOCUS Exported 15876 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulation. ACCESSION . VERSION . KEYWORDS RabV CVS-N2c(deltaG)-mCherry-P2A-Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15876) AUTHORS Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A TITLE Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. JOURNAL Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. PUBMED 26804990 REFERENCE 2 (bases 1 to 15876) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15876 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 3319..3328 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3325..4032 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(3470..3488) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3738..3757 /label=mCherry-F /note="mCherry, forward primer" CDS 4042..4098 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 4099..5130 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" CDS 7305..7316 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS complement(9108..9119) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(12316..12334) /label=T7 Term /note="T7 terminator, reverse primer" terminator 12330..12377 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(12405..12421) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(12405..12421) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(12418..12440) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 12429..12445 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(12453..12483) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12498..12519 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(12636..12653) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12807..13395) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12887..12906) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13566..14426) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 14189..14208 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(14427..14531) /gene="bla" /label=AmpR promoter rep_origin 14558..15013 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(14645..14664) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 14855..14876 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 15139..15161 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 15153..15170 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 15154..15170 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 15240..15543 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 15544..15747 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 15698..15718 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 15792..15811 /label=T7 /note="T7 promoter, forward primer" promoter 15792..15810 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa 61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt 121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa 181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt 241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc 301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga 421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca 481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa 541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc 601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg 661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc 781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat 841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca 901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg 1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga 1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag 1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc 1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg 1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg 1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca 1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact 1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa 1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta 1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc 1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga 1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca 2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc 2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga 2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt 2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc 2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag 2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga 2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg 2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga 2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat 2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga 2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg 2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg 2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg 2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct 3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc 3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg 3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag 3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc 3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggataa catggccatc 3361 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc 3421 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 3481 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac 3541 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc 3601 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg 3661 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc 3721 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc 3781 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg 3841 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg 3901 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac 3961 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac 4021 gagctgtaca agggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg 4081 gaggagaacc ctggacctat gtccaattta ctgaccgtac accaaaattt gcctgcatta 4141 ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc 4201 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca 4261 tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat 4321 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag 4381 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca 4441 ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct 4501 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc 4561 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata 4621 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta 4681 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc 4741 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg 4801 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc 4861 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc 4921 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga 4981 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc 5041 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca 5101 atggtgcgcc tgctggaaga tggcgattaa gctagcggcg cctagccggt catccttttg 5161 acgattccag tcccgaggat aacctcctct cgggattggg gggaatcttt ggatccagta 5221 gtcctccttg aactccgtcc aacagggcag attcaagagt cataagactt tcattaatca 5281 tttcagttga tcagacatgg tcgtgtagat tctcttaata cgggagatct tctagcagtt 5341 tcagtgacca acggtgcttt cattctccag gaactgatac caaaggttgt ggacaggcca 5401 aggggtactt cggatgactc tgtgcttggg cacagaaaga ggtcgtagtg tgccccccga 5461 tagcggactc gacatgaatc aactaagaaa ggcaatctgc ctcccatgat ggacataagc 5521 aatagttcac aaccatcttg catctcagtg aagtgtacat aactattgag ggctgggtca 5581 tctaagcatt tcagtcgaga aaaaaactgt agaccaaaag aacaactagc aacacttctc 5641 atccagagac ccatatcaag atgctagatc cgggagaggt ttatgatgac cctattgatc 5701 caattgagtc agaggctgaa cccagaggaa cccccactgt ccccaacatc ttgaggaact 5761 ccgactacaa tctcaattct cctttgatag aggatcctgc caaactaatg ttagaatggt 5821 tgaagacagg gaacagacct tatcggatga ctttgacaga caattgctcc aggtcttaca 5881 aagttttgaa agattatttc aagaaagtag atttgggttc tctcaaagtg ggcggaactg 5941 ctgcacagtc aatggtttct ctctggttgt gtggtgccca ctctgaatca aacaggagcc 6001 ggagatgtat aaccgacttg gcccatttct attccaagtc atcccccata gagaagctat 6061 tgaattgtac gctaggaaac agaggcctga gaatcccacc agagggggtg ttaaattgcc 6121 tcgagagggt caattatgac aaggcatttg ggaggtatct ggccaacacg tattcctctt 6181 acttgttttt ccatgtaatc accttataca tgaatgcctt agactgggaa gaggaaaaga 6241 ccatcctggc attatggaaa gatataacct cagtggatac cgagaaggac ttggtcaaat 6301 tcaaagatca aatatgggga ctgttgattg tgacaaagga ctttgtttac tctcagagtt 6361 caaactgtct ttttgacaga aactacacac tgatgctaaa ggatcttttc ttgtctcgat 6421 tcaactcctt aatgattctg ctttctcccc ctgagccccg atactcagat gacttaatat 6481 ctcagctgtg ccagctatac atcgctgggg atcaagtctt gtccatgtgt gggaactccg 6541 gctatgaagt catcaaaata ttggagccat atgtcgtgaa cagtttggtc cagagggcag 6601 agaagtttag gcctctcatc caccccttgg gagactttcc tatgttcata aaagacaagg 6661 tgaatcaact tgaagggact tttggtccca gtgcaaagag gttttttagg gttttagatc 6721 aattcgacaa catacatgac ttagtatttg tgtatggctg ttacagacat tgggggcacc 6781 cctatataga ttatcggaag ggtctgtcga aactatatga tcaagttcac attaagaaag 6841 taatagataa gtcctaccag gagtgtttag caagtgactt ggccagaagg atcctcagat 6901 ggggatttga caagtactcc aaatggtatc tagattcgag attccttgcc cgagaccacc 6961 ccttgactcc ttatgtcaag acccaaacat ggccacccaa acatatagta gacttggtgg 7021 gggacacatg gcataagctc ccgatcacgc agatctttga aattcctgaa tcaatggacc 7081 cgtcagagat actggatgac aaatcacatt ctttcaccag aacaaggtta gcttcttggc 7141 tgtcagagaa ccgagggggg cctgttccta gcgagaaggt cattatcacg gccctgtcta 7201 agccacctgt caatccccga gagtttttga aatctataga cctcggagga ttgccagatg 7261 aggatttgat aattggcctc aaaccaaagg aacgggagtt gaagatcgag ggccgattct 7321 ttgctctaat gtcatggaat ctaagattat attttgtcat caccgaaaag ctcttggcca 7381 actacatttt gccacttttt gacgcactga ctatgacaga caacctgaac aaggtgttca 7441 aaaagttgat cgacagggtc accgggcaag ggcttttgga ctattctagg gtcacatacg 7501 catttcacct ggactatgag aaatggaaca atcatcaaag attggagtca acagaggatg 7561 tattctctgt cctagatcag gtgtttggat tgaagagggt gttttctaga acacacgagt 7621 tttttcagaa gtcctggatc tattattcag acagatcaga cctcattggg ttatgggagg 7681 accaaatata ttgcttggat atgtctaacg gcccaacctg ctggaatggc caagatggcg 7741 ggctagaggg cttgcggcag aagggctgga gtctagtcag tttattaatg atagatagag 7801 aatctcaaac caggaacaca agaaccaaga tactagctca aggagacaac caggttctgt 7861 gtcctacata catgttgtca ccgggattgt ctcaagaggg gcttctctat gagttagaga 7921 gcatatcaag gaatgcactc tcaatatacc gagctatcga ggaaggagca tctaagctgg 7981 ggctgatcat caagaaggaa gagaccatgt gtagttatga ctttctcata tatgggaaga 8041 cccccttatt tcgaggcaac atattggtgc ctgaatccaa aagatgggcc cgagtctctt 8101 gcatctctaa cgaccaaata gtcaacctcg ccaatataat gtcgacagta tccaccaatg 8161 cgctgacagt ggcacaacac tctcaatctc tgatcaaacc tatgagggat tttctgctca 8221 tgtcagtaca ggcagttttc cactacctgt tgtttagccc aatcctaaaa ggcagagttt 8281 ataagattct gagtgctgaa ggggagagct ttctcctagc catgtcgcgg ataatctacc 8341 tagatccttc tttgggaggg gtgtctggaa tgtctctcgg gaggttccat atacgtcagt 8401 tctcagaccc tgtctctgaa gggttgtcat tctggagaga gatctggtta ggctctcatg 8461 agtcctggat tcacgcgttg tgtcaggagg ccgggaaccc cgatcttgga gagagaacac 8521 tcgagagctt cactcgcctt ttagaagatc ctactacctt aaatatcaaa ggaggggcca 8581 gccctaccat tctactcaag gatgctatca gaaaggctct gtacgacgag gtggacaagg 8641 tggaaaattc agagtttcga gaggcaatcc tgctgtccaa gacccataga gataacttta 8701 tactcttttt aaaatctgtt gagcctctgt tccctcgatt tctcagtgag ctcttcagtt 8761 cgtctttctt gggaataccg gagtcaatca ttggactgat acaaaactcc cggacaataa 8821 gaaggcagtt tagaaagagt ctctcaagaa ctttagaaga gtccttctac aactcagaga 8881 tccacgggat taatcggata acccagacac ctcaaagggt cggaagggtg tggccttgct 8941 cttcagagag ggcggatcta cttagggaga tctcttgggg gaggaaagtg gtaggcacga 9001 cagttcctca cccttccgag atgttggagt tgtttcccaa atcctccatt tcctgcactt 9061 gtggagcaac agggggaggc aatcctagag tctctgtatc agtactcccg tccttcgatc 9121 agtcattttt ctcacggggc cccctaaagg gatacttggg ctcgtccacc tctatgtcaa 9181 cccagctatt ccatgcatgg gaaaaagtca ctaatgttca tgtggtgaaa agggctctat 9241 cgttaaaaga atctataaac tggttcatca ataggaattc caatttggct caaactctaa 9301 ttggaaacat catgtctctg acaggccctg acttccctct agaagaggcc cctgttttca 9361 aacggacagg gtcagccttg cataggttca agtctgccag atacagcgaa ggagggtatt 9421 cttctgtttg ccctaacctt ctctcccata tctctgttag tacagacact atgtctgatt 9481 tgacccaaaa cgggaagaac tatgatttca tgtttcagcc attgatgctt tatgcgcaaa 9541 catggacatc ggaactggta cagagggata caagacttag agactccacg tttcactggc 9601 accttcggtg caacagatgt gtgaggccca ttgatgatat aacactggaa acttctcaga 9661 tcttcgagtt cccggatgtg tcaaaaagga tatccaggat ggtttctgga gctgtccctc 9721 aatttcagaa gcttcctgat atccgtctaa gaccaggtga ttttgaatcc ctaagtggta 9781 gagaaaagtc tcgccatata gggtcagctc aggggctctt atactcaatc ttagtagcaa 9841 ttcacgactc aggatacaat gatgggacca tcttccctgt caacatatac ggcaaagttt 9901 cccccagaga ctatttgaga gggcttgcaa gagggatctt gatagggtcc tcgatttgct 9961 tcttgacacg aatgacaaat attaacatta acagacctct tgaattgatc tcaggggtaa 10021 tttcctatat tctcctgagg ctggataatc atccctctct gtatataatg ctcagagaac 10081 cgtctcttag aggagaaata ttctctatcc ctcagaaaat ccccgccgct tacccaacca 10141 ctatgagaga aggcaacaga tcgattttgt gttacctcca acacgtgcta cgctatgagc 10201 gagaggcaat cacggcgtcc ccggagaatg actggctgtg gatcttctca gacttcagaa 10261 gtgtgaaaat gacgtacttg accctcatta cttaccagtc tcacctccta ctccagaggg 10321 ttgagagaaa cttgtctaag agtatgagag ctactctgcg acaaatgggt tccttaatga 10381 ggcaagtgct gggtgggcac ggagaagata ccttggagtc agacgatgac attcaacgat 10441 tactaaaaga ctctttgcga aagacaaggt gggtggatca agaggtgcgc catgcagcta 10501 gaaccatgaa tggagattac agccccgaca agaaagtatc ccgtaaggta ggatgttcag 10561 aatgggtctg ctctgctcaa cagattgccg tctccacctc agccaacccg gcccctgtct 10621 cagagcttga cattagggcc ctctctaagc ggtttcaaaa ccccttgatc tcgggcctga 10681 gagtggttca gtgggcaacc ggtgcccatt ataagcttaa gcctattcta gatgatctaa 10741 atgttttccc atctctctgt cttgtaattg gagacgggtc agggggaata tcaagggcag 10801 ttctcaacat gtttccagat tctaagcttg tgttcaacag cctattggag gtgaatgatc 10861 tgatggcttc cggaacacat ccactgcctc cttcagcaat catgagtgga ggagatgaca 10921 tcatctccag agtgatagac tttgactcaa tatgggagaa accgtccgac ctgaggaact 10981 cggccacctg gagatacttc cagtcggttc aaaaacaggt caacatgtcg tatgacctca 11041 ttatttgtga tgcagaagtt actgatattg catctatcaa ccggataact ctgttgatgt 11101 ctgatttcgc attgtctata gatggaccac tttatctggt cttcaaaact tacgggacta 11161 tgctagtaaa cccggactat aaagctattc aacatctgtc aagagcgttc ccttcggtca 11221 cagggtttgt aacccaagta acttcatcct tttcttctga gctatacctc cggttctcta 11281 aacgaggaaa gtttttcagg gacgccgagt acttgacctc ttccaccctt cgagagatga 11341 gccttgtgtt gttcaattgc agcagcccca aaagtgagat gcagagagct cgttccttaa 11401 actatcaaga cctggtaagg ggatttcctg aagagatcat atcaaatcct tacaacgaaa 11461 tgatcataac tctgattgac aatgatgtag agtccttcct agtccacaag atggtggatg 11521 atcttgagct acagagggga actctgtcta aagtggctat cattatatcc atcatgatcg 11581 ttttttccaa tagagtcttc aacatttcca aacctttgac tgaccccttg ttctatccac 11641 cgtctgatcc taaaatcctg aggcacttca acatatgttg cagtactatg atgtatctat 11701 ctaccgcttt aggcgacgtc cctaacttcg caagacttca tgacctgtat aacagaccta 11761 taacttgtta cttcagaaaa caagttattc gagggaatat ttatctatct tggagttggt 11821 ccgatgatac cccagtgttc aagagagtag cctgtaattc tagcttgagt ctgtcatctc 11881 actggatcag gttgatctac aagatagtga agactaccag actcattggc agcataaaag 11941 acctatcagg agaggtagaa cgacatcttc atgggtataa cagatggatc accctcgagg 12001 atatccgatc tagatcatcc ctactagatt acagttgttt gtaagccgga tattaccgaa 12061 agcctgtgca tgctaaaatt cttgtatgat gcatcttgaa aaaaacaaga tcttgaatcc 12121 ggacctctgg ttgtttgatt gttttttcca tctttattgt ttttttgtta agcgtgggtc 12181 ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc 12241 actcggatgg ctaagggagg ggcggggatc cggctgctaa caaagcccga aaggaagctg 12301 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg 12361 tcttgagggg ttttttgctg aaagtcgcgc ttggcgtaat catggtcata gctgtttcct 12421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 12481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 12541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 12601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 12661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 12721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 12781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 12841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 12901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 12961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 13021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 13081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 13141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 13201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 13261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 13321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 13381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 13441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 13501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 13561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 13621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 13681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 13741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 13801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 13861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 13921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 13981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 14041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 14101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 14161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 14221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 14281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 14341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 14401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 14461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 14521 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 14581 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 14641 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 14701 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 14761 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 14821 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 14881 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 14941 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 15001 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 15061 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 15121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcct 15181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 15241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 15301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 15361 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 15421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 15481 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 15541 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 15601 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 15661 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 15721 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg 15781 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagat taagcgtctg 15841 atgagtccgt gaggacgaaa cccggcgtac cgggtc //