LOCUS       Exported               14781 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses mCherry for monosynaptic circuit mapping .
ACCESSION   .
VERSION     .
KEYWORDS    RabV CVS-N2c(deltaG)-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      3319..3328
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3325..4035
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3470..3488)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3738..3757
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6210..6221
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8013..8024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11221..11239)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11235..11282
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11310..11326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11310..11326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11323..11345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11334..11350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11358..11388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11403..11424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11541..11558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11712..12300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11792..11811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12471..13331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13094..13113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13332..13436)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13463..13918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13550..13569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13760..13781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14044..14066
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14058..14075
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14059..14075
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14145..14448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14449..14652
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14603..14623
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     14697..14716
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14697..14715
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggataa catggccatc
     3361 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3421 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3481 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3541 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     3601 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3661 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3721 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3781 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3841 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3901 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3961 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4021 gagctgtaca agtaagctag cggcgcctag ccggtcatcc ttttgacgat tccagtcccg
     4081 aggataacct cctctcggga ttggggggaa tctttggatc cagtagtcct ccttgaactc
     4141 cgtccaacag ggcagattca agagtcataa gactttcatt aatcatttca gttgatcaga
     4201 catggtcgtg tagattctct taatacggga gatcttctag cagtttcagt gaccaacggt
     4261 gctttcattc tccaggaact gataccaaag gttgtggaca ggccaagggg tacttcggat
     4321 gactctgtgc ttgggcacag aaagaggtcg tagtgtgccc cccgatagcg gactcgacat
     4381 gaatcaacta agaaaggcaa tctgcctccc atgatggaca taagcaatag ttcacaacca
     4441 tcttgcatct cagtgaagtg tacataacta ttgagggctg ggtcatctaa gcatttcagt
     4501 cgagaaaaaa actgtagacc aaaagaacaa ctagcaacac ttctcatcca gagacccata
     4561 tcaagatgct agatccggga gaggtttatg atgaccctat tgatccaatt gagtcagagg
     4621 ctgaacccag aggaaccccc actgtcccca acatcttgag gaactccgac tacaatctca
     4681 attctccttt gatagaggat cctgccaaac taatgttaga atggttgaag acagggaaca
     4741 gaccttatcg gatgactttg acagacaatt gctccaggtc ttacaaagtt ttgaaagatt
     4801 atttcaagaa agtagatttg ggttctctca aagtgggcgg aactgctgca cagtcaatgg
     4861 tttctctctg gttgtgtggt gcccactctg aatcaaacag gagccggaga tgtataaccg
     4921 acttggccca tttctattcc aagtcatccc ccatagagaa gctattgaat tgtacgctag
     4981 gaaacagagg cctgagaatc ccaccagagg gggtgttaaa ttgcctcgag agggtcaatt
     5041 atgacaaggc atttgggagg tatctggcca acacgtattc ctcttacttg tttttccatg
     5101 taatcacctt atacatgaat gccttagact gggaagagga aaagaccatc ctggcattat
     5161 ggaaagatat aacctcagtg gataccgaga aggacttggt caaattcaaa gatcaaatat
     5221 ggggactgtt gattgtgaca aaggactttg tttactctca gagttcaaac tgtctttttg
     5281 acagaaacta cacactgatg ctaaaggatc ttttcttgtc tcgattcaac tccttaatga
     5341 ttctgctttc tccccctgag ccccgatact cagatgactt aatatctcag ctgtgccagc
     5401 tatacatcgc tggggatcaa gtcttgtcca tgtgtgggaa ctccggctat gaagtcatca
     5461 aaatattgga gccatatgtc gtgaacagtt tggtccagag ggcagagaag tttaggcctc
     5521 tcatccaccc cttgggagac tttcctatgt tcataaaaga caaggtgaat caacttgaag
     5581 ggacttttgg tcccagtgca aagaggtttt ttagggtttt agatcaattc gacaacatac
     5641 atgacttagt atttgtgtat ggctgttaca gacattgggg gcacccctat atagattatc
     5701 ggaagggtct gtcgaaacta tatgatcaag ttcacattaa gaaagtaata gataagtcct
     5761 accaggagtg tttagcaagt gacttggcca gaaggatcct cagatgggga tttgacaagt
     5821 actccaaatg gtatctagat tcgagattcc ttgcccgaga ccaccccttg actccttatg
     5881 tcaagaccca aacatggcca cccaaacata tagtagactt ggtgggggac acatggcata
     5941 agctcccgat cacgcagatc tttgaaattc ctgaatcaat ggacccgtca gagatactgg
     6001 atgacaaatc acattctttc accagaacaa ggttagcttc ttggctgtca gagaaccgag
     6061 gggggcctgt tcctagcgag aaggtcatta tcacggccct gtctaagcca cctgtcaatc
     6121 cccgagagtt tttgaaatct atagacctcg gaggattgcc agatgaggat ttgataattg
     6181 gcctcaaacc aaaggaacgg gagttgaaga tcgagggccg attctttgct ctaatgtcat
     6241 ggaatctaag attatatttt gtcatcaccg aaaagctctt ggccaactac attttgccac
     6301 tttttgacgc actgactatg acagacaacc tgaacaaggt gttcaaaaag ttgatcgaca
     6361 gggtcaccgg gcaagggctt ttggactatt ctagggtcac atacgcattt cacctggact
     6421 atgagaaatg gaacaatcat caaagattgg agtcaacaga ggatgtattc tctgtcctag
     6481 atcaggtgtt tggattgaag agggtgtttt ctagaacaca cgagtttttt cagaagtcct
     6541 ggatctatta ttcagacaga tcagacctca ttgggttatg ggaggaccaa atatattgct
     6601 tggatatgtc taacggccca acctgctgga atggccaaga tggcgggcta gagggcttgc
     6661 ggcagaaggg ctggagtcta gtcagtttat taatgataga tagagaatct caaaccagga
     6721 acacaagaac caagatacta gctcaaggag acaaccaggt tctgtgtcct acatacatgt
     6781 tgtcaccggg attgtctcaa gaggggcttc tctatgagtt agagagcata tcaaggaatg
     6841 cactctcaat ataccgagct atcgaggaag gagcatctaa gctggggctg atcatcaaga
     6901 aggaagagac catgtgtagt tatgactttc tcatatatgg gaagaccccc ttatttcgag
     6961 gcaacatatt ggtgcctgaa tccaaaagat gggcccgagt ctcttgcatc tctaacgacc
     7021 aaatagtcaa cctcgccaat ataatgtcga cagtatccac caatgcgctg acagtggcac
     7081 aacactctca atctctgatc aaacctatga gggattttct gctcatgtca gtacaggcag
     7141 ttttccacta cctgttgttt agcccaatcc taaaaggcag agtttataag attctgagtg
     7201 ctgaagggga gagctttctc ctagccatgt cgcggataat ctacctagat ccttctttgg
     7261 gaggggtgtc tggaatgtct ctcgggaggt tccatatacg tcagttctca gaccctgtct
     7321 ctgaagggtt gtcattctgg agagagatct ggttaggctc tcatgagtcc tggattcacg
     7381 cgttgtgtca ggaggccggg aaccccgatc ttggagagag aacactcgag agcttcactc
     7441 gccttttaga agatcctact accttaaata tcaaaggagg ggccagccct accattctac
     7501 tcaaggatgc tatcagaaag gctctgtacg acgaggtgga caaggtggaa aattcagagt
     7561 ttcgagaggc aatcctgctg tccaagaccc atagagataa ctttatactc tttttaaaat
     7621 ctgttgagcc tctgttccct cgatttctca gtgagctctt cagttcgtct ttcttgggaa
     7681 taccggagtc aatcattgga ctgatacaaa actcccggac aataagaagg cagtttagaa
     7741 agagtctctc aagaacttta gaagagtcct tctacaactc agagatccac gggattaatc
     7801 ggataaccca gacacctcaa agggtcggaa gggtgtggcc ttgctcttca gagagggcgg
     7861 atctacttag ggagatctct tgggggagga aagtggtagg cacgacagtt cctcaccctt
     7921 ccgagatgtt ggagttgttt cccaaatcct ccatttcctg cacttgtgga gcaacagggg
     7981 gaggcaatcc tagagtctct gtatcagtac tcccgtcctt cgatcagtca tttttctcac
     8041 ggggccccct aaagggatac ttgggctcgt ccacctctat gtcaacccag ctattccatg
     8101 catgggaaaa agtcactaat gttcatgtgg tgaaaagggc tctatcgtta aaagaatcta
     8161 taaactggtt catcaatagg aattccaatt tggctcaaac tctaattgga aacatcatgt
     8221 ctctgacagg ccctgacttc cctctagaag aggcccctgt tttcaaacgg acagggtcag
     8281 ccttgcatag gttcaagtct gccagataca gcgaaggagg gtattcttct gtttgcccta
     8341 accttctctc ccatatctct gttagtacag acactatgtc tgatttgacc caaaacggga
     8401 agaactatga tttcatgttt cagccattga tgctttatgc gcaaacatgg acatcggaac
     8461 tggtacagag ggatacaaga cttagagact ccacgtttca ctggcacctt cggtgcaaca
     8521 gatgtgtgag gcccattgat gatataacac tggaaacttc tcagatcttc gagttcccgg
     8581 atgtgtcaaa aaggatatcc aggatggttt ctggagctgt ccctcaattt cagaagcttc
     8641 ctgatatccg tctaagacca ggtgattttg aatccctaag tggtagagaa aagtctcgcc
     8701 atatagggtc agctcagggg ctcttatact caatcttagt agcaattcac gactcaggat
     8761 acaatgatgg gaccatcttc cctgtcaaca tatacggcaa agtttccccc agagactatt
     8821 tgagagggct tgcaagaggg atcttgatag ggtcctcgat ttgcttcttg acacgaatga
     8881 caaatattaa cattaacaga cctcttgaat tgatctcagg ggtaatttcc tatattctcc
     8941 tgaggctgga taatcatccc tctctgtata taatgctcag agaaccgtct cttagaggag
     9001 aaatattctc tatccctcag aaaatccccg ccgcttaccc aaccactatg agagaaggca
     9061 acagatcgat tttgtgttac ctccaacacg tgctacgcta tgagcgagag gcaatcacgg
     9121 cgtccccgga gaatgactgg ctgtggatct tctcagactt cagaagtgtg aaaatgacgt
     9181 acttgaccct cattacttac cagtctcacc tcctactcca gagggttgag agaaacttgt
     9241 ctaagagtat gagagctact ctgcgacaaa tgggttcctt aatgaggcaa gtgctgggtg
     9301 ggcacggaga agataccttg gagtcagacg atgacattca acgattacta aaagactctt
     9361 tgcgaaagac aaggtgggtg gatcaagagg tgcgccatgc agctagaacc atgaatggag
     9421 attacagccc cgacaagaaa gtatcccgta aggtaggatg ttcagaatgg gtctgctctg
     9481 ctcaacagat tgccgtctcc acctcagcca acccggcccc tgtctcagag cttgacatta
     9541 gggccctctc taagcggttt caaaacccct tgatctcggg cctgagagtg gttcagtggg
     9601 caaccggtgc ccattataag cttaagccta ttctagatga tctaaatgtt ttcccatctc
     9661 tctgtcttgt aattggagac gggtcagggg gaatatcaag ggcagttctc aacatgtttc
     9721 cagattctaa gcttgtgttc aacagcctat tggaggtgaa tgatctgatg gcttccggaa
     9781 cacatccact gcctccttca gcaatcatga gtggaggaga tgacatcatc tccagagtga
     9841 tagactttga ctcaatatgg gagaaaccgt ccgacctgag gaactcggcc acctggagat
     9901 acttccagtc ggttcaaaaa caggtcaaca tgtcgtatga cctcattatt tgtgatgcag
     9961 aagttactga tattgcatct atcaaccgga taactctgtt gatgtctgat ttcgcattgt
    10021 ctatagatgg accactttat ctggtcttca aaacttacgg gactatgcta gtaaacccgg
    10081 actataaagc tattcaacat ctgtcaagag cgttcccttc ggtcacaggg tttgtaaccc
    10141 aagtaacttc atccttttct tctgagctat acctccggtt ctctaaacga ggaaagtttt
    10201 tcagggacgc cgagtacttg acctcttcca cccttcgaga gatgagcctt gtgttgttca
    10261 attgcagcag ccccaaaagt gagatgcaga gagctcgttc cttaaactat caagacctgg
    10321 taaggggatt tcctgaagag atcatatcaa atccttacaa cgaaatgatc ataactctga
    10381 ttgacaatga tgtagagtcc ttcctagtcc acaagatggt ggatgatctt gagctacaga
    10441 ggggaactct gtctaaagtg gctatcatta tatccatcat gatcgttttt tccaatagag
    10501 tcttcaacat ttccaaacct ttgactgacc ccttgttcta tccaccgtct gatcctaaaa
    10561 tcctgaggca cttcaacata tgttgcagta ctatgatgta tctatctacc gctttaggcg
    10621 acgtccctaa cttcgcaaga cttcatgacc tgtataacag acctataact tgttacttca
    10681 gaaaacaagt tattcgaggg aatatttatc tatcttggag ttggtccgat gataccccag
    10741 tgttcaagag agtagcctgt aattctagct tgagtctgtc atctcactgg atcaggttga
    10801 tctacaagat agtgaagact accagactca ttggcagcat aaaagaccta tcaggagagg
    10861 tagaacgaca tcttcatggg tataacagat ggatcaccct cgaggatatc cgatctagat
    10921 catccctact agattacagt tgtttgtaag ccggatatta ccgaaagcct gtgcatgcta
    10981 aaattcttgt atgatgcatc ttgaaaaaaa caagatcttg aatccggacc tctggttgtt
    11041 tgattgtttt ttccatcttt attgtttttt tgttaagcgt gggtcggcat ggcatctcca
    11101 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgca cgtccactcg gatggctaag
    11161 ggaggggcgg ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
    11221 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
    11281 tgctgaaagt cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11341 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11401 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11461 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11521 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11581 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11641 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11701 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11761 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11821 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11881 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11941 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    12001 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    12061 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12121 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    12181 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12241 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    13021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    13081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13381 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13441 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    13501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    13561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    13621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    13681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    13741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    13801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    13861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
    13921 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    13981 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    14041 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctagtta ttaatagtaa
    14101 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    14161 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    14221 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    14281 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    14341 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    14401 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    14461 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    14521 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    14581 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    14641 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
    14701 acgactcact atagggagac ccaagctggc tagattaagc gtctgatgag tccgtgagga
    14761 cgaaacccgg cgtaccgggt c
//
