LOCUS Exported 15501 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses tdTomato for monosynaptic circuit mapping . ACCESSION . VERSION . KEYWORDS RabV CVS-N2c(deltaG)-tdTomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15501) AUTHORS Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A TITLE Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. JOURNAL Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. PUBMED 26804990 REFERENCE 2 (bases 1 to 15501) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15501 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 3319..3328 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3325..4755 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" CDS 6930..6941 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS complement(8733..8744) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(11941..11959) /label=T7 Term /note="T7 terminator, reverse primer" terminator 11955..12002 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(12030..12046) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(12030..12046) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(12043..12065) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 12054..12070 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(12078..12108) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12123..12144 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(12261..12278) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12432..13020) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12512..12531) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13191..14051) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 13814..13833 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(14052..14156) /gene="bla" /label=AmpR promoter rep_origin 14183..14638 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(14270..14289) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 14480..14501 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 14764..14786 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 14778..14795 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 14779..14795 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 14865..15168 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 15169..15372 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 15323..15343 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 15417..15436 /label=T7 /note="T7 promoter, forward primer" promoter 15417..15435 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa 61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt 121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa 181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt 241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc 301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc 361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga 421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca 481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa 541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc 601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg 661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat 721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc 781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat 841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca 901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa 961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg 1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga 1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag 1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc 1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg 1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa 1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc 1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg 1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca 1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc 1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca 1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact 1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa 1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta 1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa 1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc 1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga 1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca 2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc 2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc 2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga 2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa 2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt 2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc 2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag 2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga 2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg 2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga 2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat 2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga 2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg 2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg 2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg 2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct 3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac 3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc 3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg 3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag 3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc 3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc 3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc 3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc 3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg 3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc 3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc 3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc 3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac 3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc 3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc 3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg 3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc 4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa 4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc 4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc 4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag 4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag 4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc 4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc 4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac 4561 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg 4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc 4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac 4741 gagctgtaca agtaagctag cggcgcctag ccggtcatcc ttttgacgat tccagtcccg 4801 aggataacct cctctcggga ttggggggaa tctttggatc cagtagtcct ccttgaactc 4861 cgtccaacag ggcagattca agagtcataa gactttcatt aatcatttca gttgatcaga 4921 catggtcgtg tagattctct taatacggga gatcttctag cagtttcagt gaccaacggt 4981 gctttcattc tccaggaact gataccaaag gttgtggaca ggccaagggg tacttcggat 5041 gactctgtgc ttgggcacag aaagaggtcg tagtgtgccc cccgatagcg gactcgacat 5101 gaatcaacta agaaaggcaa tctgcctccc atgatggaca taagcaatag ttcacaacca 5161 tcttgcatct cagtgaagtg tacataacta ttgagggctg ggtcatctaa gcatttcagt 5221 cgagaaaaaa actgtagacc aaaagaacaa ctagcaacac ttctcatcca gagacccata 5281 tcaagatgct agatccggga gaggtttatg atgaccctat tgatccaatt gagtcagagg 5341 ctgaacccag aggaaccccc actgtcccca acatcttgag gaactccgac tacaatctca 5401 attctccttt gatagaggat cctgccaaac taatgttaga atggttgaag acagggaaca 5461 gaccttatcg gatgactttg acagacaatt gctccaggtc ttacaaagtt ttgaaagatt 5521 atttcaagaa agtagatttg ggttctctca aagtgggcgg aactgctgca cagtcaatgg 5581 tttctctctg gttgtgtggt gcccactctg aatcaaacag gagccggaga tgtataaccg 5641 acttggccca tttctattcc aagtcatccc ccatagagaa gctattgaat tgtacgctag 5701 gaaacagagg cctgagaatc ccaccagagg gggtgttaaa ttgcctcgag agggtcaatt 5761 atgacaaggc atttgggagg tatctggcca acacgtattc ctcttacttg tttttccatg 5821 taatcacctt atacatgaat gccttagact gggaagagga aaagaccatc ctggcattat 5881 ggaaagatat aacctcagtg gataccgaga aggacttggt caaattcaaa gatcaaatat 5941 ggggactgtt gattgtgaca aaggactttg tttactctca gagttcaaac tgtctttttg 6001 acagaaacta cacactgatg ctaaaggatc ttttcttgtc tcgattcaac tccttaatga 6061 ttctgctttc tccccctgag ccccgatact cagatgactt aatatctcag ctgtgccagc 6121 tatacatcgc tggggatcaa gtcttgtcca tgtgtgggaa ctccggctat gaagtcatca 6181 aaatattgga gccatatgtc gtgaacagtt tggtccagag ggcagagaag tttaggcctc 6241 tcatccaccc cttgggagac tttcctatgt tcataaaaga caaggtgaat caacttgaag 6301 ggacttttgg tcccagtgca aagaggtttt ttagggtttt agatcaattc gacaacatac 6361 atgacttagt atttgtgtat ggctgttaca gacattgggg gcacccctat atagattatc 6421 ggaagggtct gtcgaaacta tatgatcaag ttcacattaa gaaagtaata gataagtcct 6481 accaggagtg tttagcaagt gacttggcca gaaggatcct cagatgggga tttgacaagt 6541 actccaaatg gtatctagat tcgagattcc ttgcccgaga ccaccccttg actccttatg 6601 tcaagaccca aacatggcca cccaaacata tagtagactt ggtgggggac acatggcata 6661 agctcccgat cacgcagatc tttgaaattc ctgaatcaat ggacccgtca gagatactgg 6721 atgacaaatc acattctttc accagaacaa ggttagcttc ttggctgtca gagaaccgag 6781 gggggcctgt tcctagcgag aaggtcatta tcacggccct gtctaagcca cctgtcaatc 6841 cccgagagtt tttgaaatct atagacctcg gaggattgcc agatgaggat ttgataattg 6901 gcctcaaacc aaaggaacgg gagttgaaga tcgagggccg attctttgct ctaatgtcat 6961 ggaatctaag attatatttt gtcatcaccg aaaagctctt ggccaactac attttgccac 7021 tttttgacgc actgactatg acagacaacc tgaacaaggt gttcaaaaag ttgatcgaca 7081 gggtcaccgg gcaagggctt ttggactatt ctagggtcac atacgcattt cacctggact 7141 atgagaaatg gaacaatcat caaagattgg agtcaacaga ggatgtattc tctgtcctag 7201 atcaggtgtt tggattgaag agggtgtttt ctagaacaca cgagtttttt cagaagtcct 7261 ggatctatta ttcagacaga tcagacctca ttgggttatg ggaggaccaa atatattgct 7321 tggatatgtc taacggccca acctgctgga atggccaaga tggcgggcta gagggcttgc 7381 ggcagaaggg ctggagtcta gtcagtttat taatgataga tagagaatct caaaccagga 7441 acacaagaac caagatacta gctcaaggag acaaccaggt tctgtgtcct acatacatgt 7501 tgtcaccggg attgtctcaa gaggggcttc tctatgagtt agagagcata tcaaggaatg 7561 cactctcaat ataccgagct atcgaggaag gagcatctaa gctggggctg atcatcaaga 7621 aggaagagac catgtgtagt tatgactttc tcatatatgg gaagaccccc ttatttcgag 7681 gcaacatatt ggtgcctgaa tccaaaagat gggcccgagt ctcttgcatc tctaacgacc 7741 aaatagtcaa cctcgccaat ataatgtcga cagtatccac caatgcgctg acagtggcac 7801 aacactctca atctctgatc aaacctatga gggattttct gctcatgtca gtacaggcag 7861 ttttccacta cctgttgttt agcccaatcc taaaaggcag agtttataag attctgagtg 7921 ctgaagggga gagctttctc ctagccatgt cgcggataat ctacctagat ccttctttgg 7981 gaggggtgtc tggaatgtct ctcgggaggt tccatatacg tcagttctca gaccctgtct 8041 ctgaagggtt gtcattctgg agagagatct ggttaggctc tcatgagtcc tggattcacg 8101 cgttgtgtca ggaggccggg aaccccgatc ttggagagag aacactcgag agcttcactc 8161 gccttttaga agatcctact accttaaata tcaaaggagg ggccagccct accattctac 8221 tcaaggatgc tatcagaaag gctctgtacg acgaggtgga caaggtggaa aattcagagt 8281 ttcgagaggc aatcctgctg tccaagaccc atagagataa ctttatactc tttttaaaat 8341 ctgttgagcc tctgttccct cgatttctca gtgagctctt cagttcgtct ttcttgggaa 8401 taccggagtc aatcattgga ctgatacaaa actcccggac aataagaagg cagtttagaa 8461 agagtctctc aagaacttta gaagagtcct tctacaactc agagatccac gggattaatc 8521 ggataaccca gacacctcaa agggtcggaa gggtgtggcc ttgctcttca gagagggcgg 8581 atctacttag ggagatctct tgggggagga aagtggtagg cacgacagtt cctcaccctt 8641 ccgagatgtt ggagttgttt cccaaatcct ccatttcctg cacttgtgga gcaacagggg 8701 gaggcaatcc tagagtctct gtatcagtac tcccgtcctt cgatcagtca tttttctcac 8761 ggggccccct aaagggatac ttgggctcgt ccacctctat gtcaacccag ctattccatg 8821 catgggaaaa agtcactaat gttcatgtgg tgaaaagggc tctatcgtta aaagaatcta 8881 taaactggtt catcaatagg aattccaatt tggctcaaac tctaattgga aacatcatgt 8941 ctctgacagg ccctgacttc cctctagaag aggcccctgt tttcaaacgg acagggtcag 9001 ccttgcatag gttcaagtct gccagataca gcgaaggagg gtattcttct gtttgcccta 9061 accttctctc ccatatctct gttagtacag acactatgtc tgatttgacc caaaacggga 9121 agaactatga tttcatgttt cagccattga tgctttatgc gcaaacatgg acatcggaac 9181 tggtacagag ggatacaaga cttagagact ccacgtttca ctggcacctt cggtgcaaca 9241 gatgtgtgag gcccattgat gatataacac tggaaacttc tcagatcttc gagttcccgg 9301 atgtgtcaaa aaggatatcc aggatggttt ctggagctgt ccctcaattt cagaagcttc 9361 ctgatatccg tctaagacca ggtgattttg aatccctaag tggtagagaa aagtctcgcc 9421 atatagggtc agctcagggg ctcttatact caatcttagt agcaattcac gactcaggat 9481 acaatgatgg gaccatcttc cctgtcaaca tatacggcaa agtttccccc agagactatt 9541 tgagagggct tgcaagaggg atcttgatag ggtcctcgat ttgcttcttg acacgaatga 9601 caaatattaa cattaacaga cctcttgaat tgatctcagg ggtaatttcc tatattctcc 9661 tgaggctgga taatcatccc tctctgtata taatgctcag agaaccgtct cttagaggag 9721 aaatattctc tatccctcag aaaatccccg ccgcttaccc aaccactatg agagaaggca 9781 acagatcgat tttgtgttac ctccaacacg tgctacgcta tgagcgagag gcaatcacgg 9841 cgtccccgga gaatgactgg ctgtggatct tctcagactt cagaagtgtg aaaatgacgt 9901 acttgaccct cattacttac cagtctcacc tcctactcca gagggttgag agaaacttgt 9961 ctaagagtat gagagctact ctgcgacaaa tgggttcctt aatgaggcaa gtgctgggtg 10021 ggcacggaga agataccttg gagtcagacg atgacattca acgattacta aaagactctt 10081 tgcgaaagac aaggtgggtg gatcaagagg tgcgccatgc agctagaacc atgaatggag 10141 attacagccc cgacaagaaa gtatcccgta aggtaggatg ttcagaatgg gtctgctctg 10201 ctcaacagat tgccgtctcc acctcagcca acccggcccc tgtctcagag cttgacatta 10261 gggccctctc taagcggttt caaaacccct tgatctcggg cctgagagtg gttcagtggg 10321 caaccggtgc ccattataag cttaagccta ttctagatga tctaaatgtt ttcccatctc 10381 tctgtcttgt aattggagac gggtcagggg gaatatcaag ggcagttctc aacatgtttc 10441 cagattctaa gcttgtgttc aacagcctat tggaggtgaa tgatctgatg gcttccggaa 10501 cacatccact gcctccttca gcaatcatga gtggaggaga tgacatcatc tccagagtga 10561 tagactttga ctcaatatgg gagaaaccgt ccgacctgag gaactcggcc acctggagat 10621 acttccagtc ggttcaaaaa caggtcaaca tgtcgtatga cctcattatt tgtgatgcag 10681 aagttactga tattgcatct atcaaccgga taactctgtt gatgtctgat ttcgcattgt 10741 ctatagatgg accactttat ctggtcttca aaacttacgg gactatgcta gtaaacccgg 10801 actataaagc tattcaacat ctgtcaagag cgttcccttc ggtcacaggg tttgtaaccc 10861 aagtaacttc atccttttct tctgagctat acctccggtt ctctaaacga ggaaagtttt 10921 tcagggacgc cgagtacttg acctcttcca cccttcgaga gatgagcctt gtgttgttca 10981 attgcagcag ccccaaaagt gagatgcaga gagctcgttc cttaaactat caagacctgg 11041 taaggggatt tcctgaagag atcatatcaa atccttacaa cgaaatgatc ataactctga 11101 ttgacaatga tgtagagtcc ttcctagtcc acaagatggt ggatgatctt gagctacaga 11161 ggggaactct gtctaaagtg gctatcatta tatccatcat gatcgttttt tccaatagag 11221 tcttcaacat ttccaaacct ttgactgacc ccttgttcta tccaccgtct gatcctaaaa 11281 tcctgaggca cttcaacata tgttgcagta ctatgatgta tctatctacc gctttaggcg 11341 acgtccctaa cttcgcaaga cttcatgacc tgtataacag acctataact tgttacttca 11401 gaaaacaagt tattcgaggg aatatttatc tatcttggag ttggtccgat gataccccag 11461 tgttcaagag agtagcctgt aattctagct tgagtctgtc atctcactgg atcaggttga 11521 tctacaagat agtgaagact accagactca ttggcagcat aaaagaccta tcaggagagg 11581 tagaacgaca tcttcatggg tataacagat ggatcaccct cgaggatatc cgatctagat 11641 catccctact agattacagt tgtttgtaag ccggatatta ccgaaagcct gtgcatgcta 11701 aaattcttgt atgatgcatc ttgaaaaaaa caagatcttg aatccggacc tctggttgtt 11761 tgattgtttt ttccatcttt attgtttttt tgttaagcgt gggtcggcat ggcatctcca 11821 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgca cgtccactcg gatggctaag 11881 ggaggggcgg ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca 11941 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt 12001 tgctgaaagt cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 12061 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 12121 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 12181 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 12241 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 12301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 12361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 12421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 12481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 12541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 12601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 12661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 12721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 12781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 12841 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 12901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 12961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 13021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 13081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 13141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 13201 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 13261 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 13321 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 13381 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 13441 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 13501 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 13561 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 13621 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 13681 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 13741 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 13801 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 13861 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 13921 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 13981 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 14041 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 14101 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 14161 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 14221 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 14281 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 14341 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 14401 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 14461 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 14521 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 14581 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc 14641 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 14701 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 14761 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctagtta ttaatagtaa 14821 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 14881 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 14941 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 15001 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 15061 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 15121 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 15181 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 15241 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 15301 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 15361 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat 15421 acgactcact atagggagac ccaagctggc tagattaagc gtctgatgag tccgtgagga 15481 cgaaacccgg cgtaccgggt c //