LOCUS       Exported                9596 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  gRNA expression vector (with mKate2).
ACCESSION   .
VERSION     .
KEYWORDS    pgRNA-CKB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    309..426
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    679..712
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     716..736
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        1053..1356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1358..1635
                     /label=chicken beta-actin promoter
     intron          1637..2654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2578..2600
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2662..2681
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2719..2739
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2743..2763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2767..2787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2803..3498
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             3499..3552
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3996..4015)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4250..4283
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4339..4927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4392..4412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4810..4821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4930..4946)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4931..4947)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5456..5636
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5698..6286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5778..5797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6457..7317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7080..7099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7318..7422)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7506..7525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7697..8076
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8078..8276
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8231..8251
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8294..8474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8521..8646
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9145..9378
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9563..11
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
       61 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
      121 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
      181 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      241 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      301 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      361 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      421 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      481 tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga cttgtgggag
      541 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
      601 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
      661 tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt ataaacagca
      721 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aagtatccct
      781 tggagaacca ccttgttggg gagacggacg tctccgttta agagctatgc tggaaacagc
      841 atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      901 ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt attaccgcca
      961 tgcatcccgg gtgactgact gagcggccgc actagttatt aatagtaatc aattacgggg
     1021 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1081 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1141 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1201 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1261 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1321 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     1381 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     1441 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
     1501 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     1561 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     1621 agcgcgcggc gggcggggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
     1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     2701 aagaattccg ccaccatgcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     2761 gtagatccaa aaaagaagag aaaggtacac gtgtcaacgc gtatggtgag cgagctgatt
     2821 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     2881 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg
     2941 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     3001 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc
     3061 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     3121 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     3181 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
     3241 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     3301 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag
     3361 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc
     3421 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3481 agcaaactgg ggcacagaga gggcagagga agtcttctaa catgcggtga cgtggaggag
     3541 aatcccggcc ctatgaagac cttcaacatc tcccagcagg atctagaatt agtagaagta
     3601 gcgacagaga agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt
     3661 cgtacgaaaa caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta
     3721 actgtttgtg cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt
     3781 gacacgattg tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg
     3841 gtaagtcctt gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg
     3901 ttaatagaaa tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa
     3961 tatacccgaa attaatcgac atgataagat acattgatga gtttggacaa accacaacta
     4021 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtga
     4081 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4141 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     4201 caagtaaaac ctctacaaat gtggtagatc gaattcgtcg agggacctaa taacttcgta
     4261 tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc
     4321 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4381 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4441 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4501 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4561 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4621 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4681 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4741 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4801 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4861 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4921 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     4981 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     5041 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     5101 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5161 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     5221 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     5281 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     5341 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     5401 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     5461 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5521 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5581 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     5641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6121 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7441 gccacctgac gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact
     7501 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7561 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     7621 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     7681 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7741 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7801 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7861 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7921 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7981 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     8041 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     8101 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     8161 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     8221 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     8281 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8461 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8521 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8581 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8641 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8701 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8761 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8821 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8881 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8941 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
     9001 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9061 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9121 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9181 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9241 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9301 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9361 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9421 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9481 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9541 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaat
//
