LOCUS Exported 19776 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye. . ACCESSION . VERSION . KEYWORDS pEMS1383 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 19776) AUTHORS Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM TITLE A regulatory toolbox of MiniPromoters to drive selective expression in the brain. JOURNAL Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PUBMED 20807748 REFERENCE 2 (bases 1 to 19776) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..19776 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" regulatory 3510..3519 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3516..4232 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3516..4232 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3561..3582) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3822..3841) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4169..4190 /label=EGFP-C /note="EGFP, forward primer" CDS 4245..4262 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" CDS 4275..4295 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4299..4319 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4323..4343 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 4350..4383 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 4506..4587 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4543..4562) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12232..12253 /label=F1ori-F /note="F1 origin, forward primer" promoter 12416..12520 /gene="bla" /label=AmpR promoter CDS 12521..13381 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(17588..17610) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 17696..17836 /label=bom /note="basis of mobility region from pBR322" primer_bind 17751..17770 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(17851..17868) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(18022..18610) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(18102..18121) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(18781..19641) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(19642..19746) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccca gtctggacat agagcaagac tctgtttcaa aaacaaaaac 181 aagcagagtc caagcacctc cttgagtcag agcagggcat ttcctcctgc tccgatccac 241 gacagcagca tttcatgtgg ttgggacttg gactctggag gcaggcccag gttcaaatcc 301 tggctttgac actgagtgat acaacctgag cctcagtttc ccccatcatg atggagaaac 361 atgcgtttcc tatgcaggct gaggctccga aggctgctaa gctcctttcc ctgctaaggg 421 gaggtcctca tttatgccat tgcccaggtt tcctttgcat gtgacttatc acacggtgct 481 ctggagccct gtccttggag actcacaccc ctgtgaccag gatggggtca gggctgtgct 541 taatcaggtg actgtccaag gagccccctt ccacctccag agttcagaac tcagtgtctt 601 accttcctcc caggaaccag agcacactga acacgcaggg cctggcaggg cacctgacgg 661 ccaacatgtt ggggggcatt ggagcacaca aatcaacact ccaggggcag gagtggttgt 721 cacttgagtt gcaaaatcta gggaccctaa aagagtccct gtgaggcctg gctccttggt 781 gtgctgagca actgacccat cccccacact ctcctaccca aagaggcctc tggagccttc 841 ctacggccaa gggacctggc cagccgcaca ggctgaggac agagcgagct cggccagggc 901 tccgtgaaca gctgtccttc tgtggccatc atcacagcag cagcaagttc agggtggggc 961 atcagcacgg ggtgtgcgct ctctcatcct gggcatctga gtttttcgtt ttaaaagttg 1021 tgatcaccaa gacgctgtcc ttgtctgcct ctgcatctca tggtccccgc tgtggggagg 1081 cgcgcagcca agtgggcctc gcctggggaa gggaggtgca tgcatgcgac tcaggcaaac 1141 acggcccctg ggccttgttc cagggagccc caaaggcaga gttcagaaga ttgcaattaa 1201 ttttctaaat taatacacaa taacagataa ttaattttaa atgttgaact taatgttagt 1261 acatatgtta caggaattac atcaaattat ttgattttat tatggagtct gtcttaagtt 1321 ttaattccaa aaatagttta ttcctcctat gatttaaatt ttatttacat tagcactcaa 1381 tagaactact agatgctaaa tatacctgtg gatgtattcc agttacacta aaaattgaaa 1441 tatagtgcca ggttattcac agtgttttta acttgaagtt tgtttttcaa aagcaaaaga 1501 ggttattgtt ctgcagtagc aaaactgcta gggattgagc ttccagattt tttaaaatta 1561 tatttatata tttagacgga gtttcattct tgttgcccag gctggagtgc aatggtgcag 1621 tctcagctca ctgcaacctc tgcctcctgg gttcaagcaa ttctcctgcc ttagcctctc 1681 cagcagctgg gattacaggc acctggctaa ttttttttgt atttttagta gagatggggt 1741 tttaccgtgt tggccaggct ggtcttgaac tcctaacctc aggtgatctg cccacctcgg 1801 cctcccaaag tgctaggatt acaggcgtga gccactgtgc ccaaccgagc gtccagattt 1861 taggttctca gctttcctct tctctcatac gtttggtatg ttctttattt aaatctctaa 1921 aataaaacaa ctcatttcca cctcctgggt cgttgctgtc gaggtggttc aattccacgc 1981 aggcattttt acagtcaagg cgtgagcatc ctcacttgtt aagtagagtt tgtttgtttg 2041 tttttctttg tttttttgag acagaatctc tgtgacccag gctggtcagc acttacagga 2101 atggtccctc caacatctga gcgaggaagg ggcacagagc acagttgtgc cttccttggg 2161 cctgggcaag ctctgtgctt cacagagcaa gcctgtgtca gatccttcag cagagaccac 2221 agccaggggt ggtgaagccg atgaggaagt gagggccaac cacactgctc aggctgttcc 2281 tcacaggagg ctggggcagg ctgagccacg caagaccaca gctctcagcc ctgtgctatt 2341 tccagggcac acttacagat cctgaatggt agagaacgtc ctggataaaa caatgacagc 2401 aagacagcca tgtacacaga tatcttgtgg gaaacccttt cacggccact ttgaaactgg 2461 gttgggagaa ggacgtgatg ctgggtcagg catccattgg ggaccatgga catttttgga 2521 gagtttgggc caatgttctt gtgtaatttg tcagaaggaa cacctacttc ttcattcttg 2581 tgaagaagaa cccataaact ggcccccgtg ttcagaggct ccagtcgagc gagttgaggg 2641 gacagctgga cttctccttc ctcaggctgg ctggaggctt cagcaagggg gcacaccgtc 2701 cacacgcctc tgtaaaacag cgttcgaacg ggacatctct aaggcatcgt ccaactctga 2761 gagtctgtga ccttccaccc tggggtggag ggaaaggcct tgggatacag tccacacatc 2821 acgttttccc ctaaaagtac cacccgtttt agtccctgca gacactggcc tcagcagctt 2881 ctctgaggca gccccgttga gcccagggct gaccacagac aagcactact gtccacagaa 2941 cttcacgccc agtggggcca gacttggagg atggcagagg agagaagctc caggggcctc 3001 tccattagaa accaacttgc aggccactta atctcctatg ctcagcctgt acttggaagc 3061 tgcttgttct tggctgcaca tttgctttgt ttgaatcaat taatccaagt ctctctcctc 3121 tccccacccc caaccttgcc tttagggtga catcaatatt catgtaataa agaaatcgca 3181 caaaaagctg actccccact tcctgcccta caggcccttt tttcttcctc agcccatgtg 3241 caatcctggc tcccagcaag tccccggggc tgcttggtca atgcagccct gtgtgcaggc 3301 ctggcagccc tgccaccccc gccttcggct cccattggct gccacggcct gcagtgggct 3361 gcaccagggt tcatccatcc tccctgggca gagggaataa gaggctgcct ctgcccacca 3421 gtcctgccgc ccaggacccg cagcagagac gacgcctgca gcaaggggcg cgccgaagtt 3481 cctattcttc aaaaggtata ggaacttccg ccaccatggt gagcaagggc gaggagctgt 3541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 3601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 3661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 3721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 3781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 3841 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 3901 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 3961 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 4021 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 4081 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 4141 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 4201 ggatcactct cggcatggac gagctgtaca agttatttct gaactgctgc ccgggctgct 4261 gcatggaacc ggatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag 4321 atccaaaaaa gaagagaaag gtaatataag aagttcctat tctctagaaa gtataggaac 4381 ttcagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 4441 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 4501 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 4561 tcacaaataa agcatttttt tcactgcgaa ttctcgtaaa actcttcatg gcagtagtta 4621 ttattctctc tctctctctt tttctttttt cttgagacag gatatttctc tgttgcccag 4681 gctggagtgc agtggcacag tcttggctca ctgcagcctg gacctcctgg gctcaagcca 4741 tcctcccacc tcagcctccc aagtagctgg ggctacaggc acatggccac caggcccaga 4801 taatttttca tttttgtaga gactgagtct caccatgtta cccaggttta ttattctcat 4861 tttttagatg aagagactga ggtccagaga agctcaatga cttgcctagt tttacaaatc 4921 tcctgccatc acatacccct cagcgtcctt aataagaggg aggccaccaa ctatgtgctg 4981 ggcactgtgg tggatgctgg agctataggg ttgagtatat aagaaatggt gttgctggag 5041 caactgttgc ttgcttacct gacctatctg agaattaatt agcaggggaa catatttttg 5101 ttttcagatt caatataaga acttgtgtgg gcaaaaataa agatcagtag taataacagt 5161 agttcccatt tgctgactgt actgtcctaa gtgcatatat atatacatac acacacgcat 5221 acctatactc ctctaatact caaaatgatc ctgtttatgt attgttaata tgctcatttt 5281 atttttaaat ttttatttat ttttattttt atttattttt gagacggagt ctcattctgt 5341 cgcggaggct gaagtgcagt ggtgcgatct cagctcagtg cgacctccgc ctcccgggtt 5401 caagtgattc tcctgcctca gcctccggac tagctgggat tacaggcgcc cgcctccacg 5461 cccagctaat ttttgtattt ttagtagaga tggggtttcg ccatgttggc caggctggtc 5521 tcgtactcct gaccttgagt gatccacctg cctcggcctc ccaaagtgct gggattacag 5581 gcatgagcca ccgcgccggg ctaatatgct cattttagtg aggcaaaaat agaggctcag 5641 agtctgattt gtacaaaact acagagcagt taagtgtcct ctcagatgtg taccctgatc 5701 tgggtgactc taggactcta ggtctcaact gttacaacca gttaagggtt tggggaagca 5761 ctgggccaag agtcaggaaa atggaagcca caggtagtgc aaggtcttgg gaatgggacg 5821 tctggtccaa ggattcacgc gatgactgga acccgaagag ccggggcccg gtttacggcc 5881 gccatgaagc aacgcgcgcc ggtaggtttg ggaatcaggg agccctctga ataggagact 5941 gagttgggag ggaaaggggc ttcgctgggg gagcctcggc ttcttctggg agaaaattcc 6001 cacggctacc tagtgagcct gcaaactggt aggcgccggc gtaggcgcgc gggcggggcc 6061 gggggcgggg cctgcggggc gtggcggggc gggcagaggg cggggcctgc ttctcctcag 6121 cttcaggcgg ctgcgacgag ccctcaggcg aacctctcgg ctttcccgcg cggcgccgcc 6181 tcttgctgcg cctccgcctc ctcctctgct ccgccaccgg cttcctcctc ctgagcagtc 6241 agcccgcgcg ccggccggct ccgttatggc gacccgcagc cctggcgtcg tggtgagcag 6301 ctcggcctgc cggccctggc cggttcaggc ccacgcggca ggtggcggcc gggccctgag 6361 gcgcgggatc gatccatgac acccccccgc cccccatatc tcactataat tcactgtcaa 6421 atggctttta tgatttggag tttggggata cttttaaaga cagggtcttg gtagctccta 6481 gatggcttgg gattcactta gtaacccttg ctggcctaaa attcaggaac ttctacctca 6541 tcttcccaag tactgggtta gtagggatgt ccagcatatt tagcttgctt taatgatttt 6601 gctgtacttt tttctgtgtg tacaggtgca tgtatattta taaaactaac ataatatatt 6661 ttacccccaa agtaccataa ccctaattaa tgctattaaa tgttttgtat gctgggctct 6721 gggcatacta atataaccaa tattatacaa tgcaaaacaa ttttggaata tcttctgtaa 6781 aattatctta ttagtaagtt gtaaccatct cagacttcta agtttgagtt tgtaaaggat 6841 cagttttttt tggtgaccta cacacacaca cacacacaca cacacacaca cacacacagg 6901 ggggtggatt ctgagaattt agagaattta gatggttaat accagttttt atttttattc 6961 tgtgtgatat ttatttatta agatttattt atttattaca tgtaggtaca ctgtatctgt 7021 cttcagacac accagaagag ggcatcagat ctcgttacag atggttgtga gtcaccatgt 7081 ggttgctggg atttgaactc aggacctctg gaagaacagt cagtgctctt aaccgttgag 7141 ccatctcacc agccccgtga tattttattt aacttactag aatatactgt gttaaaacat 7201 attttagggg attggggaga tggcccattc tgtaaagtgt ctgccatgtc atcttcagga 7261 cctgcgtttg taaccccagc atggagaaga cagagacagg gagacccctg ggcttcctgg 7321 caagctagac tagcaaattt ggttaactag gttcaatgag agactcagtc tcaaaacata 7381 agaagtgaat tttttagacc caatttcaat ctctgaactc catacgtatg cacatgtacc 7441 tgcatataaa tatatataca cacccataaa cacatgcaca cacaaaaaat ggttcaaaag 7501 ttcttaaaat gtataagagt agaaagtgta gagccgggag tggtggcgca cgcctttaat 7561 cccagcactt gggagcagag gcaggtggat ttctgagttc gaggccagcc tggtctacag 7621 agtgagttcc aggacagcca gagctataca gagaaatcct gcctcaaaaa accaaaaata 7681 aagtgtatat actaatcttt catgcctaaa atatttattt ttgccacatt tttcatacct 7741 ctttaatatt tttctttttc tctttgtgct tcaaaggaaa ttttgtgtca tgttttactt 7801 ttcattcttg actctattca gcagtaagac gcagcatggc tttcttagag aagtataatc 7861 tattacaaaa cggtaacatt taactgctct acataccctc ttgtagctaa attcctctgt 7921 taaactaaga cttattatct aaacgtaatt aaatgttgat atcttataat gctccacttt 7981 gaaacagctg tctttctttt gcagattagc gatgatgaac caggttatga cctagatttg 8041 ttttgtatac ctaatcatta tgccgaggat ttggaaaaag tgtttattcc tcatggactg 8101 attatggaca ggttagtaag acctcgattg aagttatttt tagagggtgt gtgtgtggtg 8161 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtaag agagagagga gaggcatgtg 8221 tgatatatat gtgaagtgta ttgcatgtac atggaatcca gagaaggaca ttaggtgtcc 8281 tactctgttg ctctcttatt cccttgaatc agtgtctctt gctgaacctg gggcatacat 8341 actgatttgg ctaggctgcc tggctagcaa gacccaggga tctgcttgtc tctgcccccc 8401 agtgctgacg ttacaagcag gtgcaaccac atctggtttt tatatgggta ctgggggatc 8461 tgaacttagg gcctgatgcg tgcccaagtg ctctcacccg ccaagccacc tctccagcca 8521 aagggttttt tttcttgtat taattctaac ttagcaactg ttgagtagtt gatgtttgaa 8581 tataagctaa gccaacttat tagaataaat gttttctatt ttttacttgt atgtgtgagt 8641 gcctgcatgt atgtatgtga agcatatgta ttccaggatg tcagaattgt gatggattcc 8701 ctggaactgg agttacagac cattgtgaac cactgtgtat gggtgctggg aactgaactt 8761 taggtcctct gcaaaaacag taaggatctt aactgataaa ctatctttct agccctaagg 8821 ctttctactt ttaaagttcg tttctctttc tctctctctc tctctctctc tctctctctc 8881 tccctctccc tccctcctcc tcctcctcct ctttcccccc tctcgcctcc tctttctctc 8941 tcccctccct ctctctctct cacacacaca cacagacaga gagaggagag agaaaggaag 9001 ccttactaat taattaccta gttttagaca taactagtgg gaatccatag taagtaaatc 9061 aacattcttg aagagaggtt agaagaattg gctctcgttg tccatcgagg ctgtataaga 9121 ttattcttct gttttctcag cttaccatac atattttcta cgtagacaat accaattcaa 9181 atacactgta cgtaaagtat atgcgcacac acacacacac acacacacac acacacacac 9241 aagttacata ttgtcatata gtactaaaat ttatggactt tttcatatta atacatctag 9301 agctatcatg ttttttatct tttaaaaatt actttacggg gctacagaga tgtctcagca 9361 gttaggagca ctgactgctc ttccagaggt cctgagttca attcccagca accacatggt 9421 ggctcacaac catctgtaat gggatctgat ccactcttat ggtgtgtctg aagacagtga 9481 cagtgtactc atatacatga aataaatctt taaaaaatta ctttaccatc agggtatata 9541 tatatatata tatgtgtgtg tgtgtgtgtg tgtgtatgaa gtatttgcta tttgagaggg 9601 tctcactgtt tagctcagtc tggcttggaa cgttctgtat agcccagact ggcctcaact 9661 cagcagtccc cctgaaaggc tctcatatgt ggagattaca ggtatgtgct actgtgccca 9721 gccctacatg ttactttgtt tttgaaatag ggtatcaaat agcttagact ggcttctaag 9781 tcagtgtgta gctaaggatg gtctgagctt ctgaacctgc tacttcaact cctggtgtgc 9841 catcatggcc agtttgcaca gttgtgagag tagaactcag gacctcctat gtgttactag 9901 acatgttctc taccagctga gctacatccc aattcgccta catttacttt tattttttaa 9961 aagatttatt tatgtataca gcactctgcc tgcatgtaca tctgcatgcc agaagagggc 10021 accagatctc atcatacata gttgtgagcc accatgtggt tgctggggat tgaactcagg 10081 gcctctggaa gagcagtcag tgctcttaac ctctgagcca tctctgtagc cccctacatt 10141 tattttaaag attataggct attctattca tagatgtgcc ataactgatt taaccagatc 10201 ttcattattt tgtacttagt agagtatatt agagggaatg aatcttaaat acacattttg 10261 acacttgctc agctttagac aagtaaaaat cactggtcaa ggaaatggtg cttagtggat 10321 gcttctgaac tgtcaaattg tcctccaaca agttacatca ttttatactt ccaacgttat 10381 gtgagagttc atttttcttt aggctcttct agtattttat atttgagaaa taaaatttaa 10441 ccaaaatttg ccaatctagt aagcttgttt ttatttgtat ctttaaattt tatgatagat 10501 gttatagtgt actctcctct cccctcccct cccctctcct cctcagcagg atcttgttat 10561 gtagccctaa gggatctaat acttactctg tagtccaggc tgtccttcag ctcatgagac 10621 acaccatcat gcttggcatg tttcttgttc tgaaaggtat ctggttttaa ttttggttga 10681 gctggtgtgt tggtgtatat ctgtaatctc agcacaggca agtagagaca aggattatga 10741 ttacagtcca gcctgggcta tttagaggga ttctctttaa tctttcctca tgccccaaaa 10801 tcttaccttt ggtatatgaa aaatagtctc cacttctgca aaatattgct ttatgaagta 10861 agaattaatt cccttcatag agacaaggaa tgtgtcctgt aaaagtttaa tgtgtaagaa 10921 gtatttgtta taaaagataa atattcagaa tcttcttttt aattcctgat tttatttcta 10981 taggactgaa agacttgctc gagatgtcat gaaggagatg ggaggccatc acattgtggc 11041 cctctgtgtg ctcaaggggg gctataagtt ctttgctgac ctgctggatt acattaaagc 11101 actgaataga aatagtgata gatccattcc tatgactgta gattttatca gactgaagag 11161 ctactgtgta agtataatta acttataatt aaaaaaatag ggccattcta gttttatcta 11221 tatttttttt aaacttgtgc aaactatgct acataatcaa cttacttgat tatgtaatat 11281 atatagtaat agttggaatt actattatta catatttatt tttctgtgct ggggtagaac 11341 ccagggaact aagtactgta ataaccgagc ttcattcatc ctagtcattt ttaatttttt 11401 tttttttttt tgttttttcg agacaaggtt tctctgtgta gccctggctg tcctggaact 11461 cactctgtag accaggctgg cctcgaactc agaaatctgc ctgcctctgc ctcccagtgc 11521 tgagattaat gacgtgcacc accactgcct ggcatttttt taaagtttta atttgagacc 11581 ctttctcact aacttgctca cactatcctc gaactcatta cgtgggctgg tctagaaatt 11641 tcagtcaact tgcctcagct tctcaagtag ctgggattac aggcaggtgc taccacttct 11701 ggccatgatc attgttgttg ttgctattat tattattatt atttcactga tacttcatat 11761 cacaacacag aatagtaact gtagttcatg accaacaaaa aagttgtaca gtttctctgt 11821 tgtcttattt tgatctggat tttgatttgt ttcatggcac tgggcaggct aatctcaaac 11881 tgcatggctc aaatagtcct tctgtcttaa tctcccatgt tctggaagta taggtatcac 11941 tacacctagc tgtattttca tattttaaaa aaaacgccta agaattgcta ttgaatgagg 12001 cggagagatg gctcagcagt cacaagtact tgtagaggac tggggtttgg ttactagcac 12061 ctcaaagatg gcttacaatt atctgtagct ccaattccag aggatctgtg tggtaaagct 12121 gcatgtatat gggcatgcag gtaaaactgc taaacatata aaaatagctc agtcttaaga 12181 gaattggtcg actttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 12241 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 12301 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 12361 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 12421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 12481 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 12541 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 12601 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 12661 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 12721 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 12781 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 12841 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 12901 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 12961 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 13021 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 13081 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 13141 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 13201 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 13261 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 13321 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 13381 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 13441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 13501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 13561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac caggtcgagt 13621 agggataaca gggtaatgga tcctcgttgg gcacctgcac tcatgtacgc aggcccacat 13681 atagatgtac acacgcatat cattcaaaat agaaattatt tatacaagta gtgcgttgga 13741 gagctggctc aggagttgtt aatgcttgct acccttgcaa aggacccaag ttcaatttcc 13801 agcacctgtg ttgggcatct cacaaccaca tgtaaatcta gtttcagggg atttcacccc 13861 tcttctgaac tcttcaggca cttataccca caaatggaca tctccacata gagacacata 13921 cttttaaaaa agtaatctag ggtctagaga aatggttata gaccacggac tgggatcagt 13981 tcccagaagc cacatcctgt ggctcacaat tgcctgtgaa tcaagttcta gatctggtgg 14041 cctcgtctag cctgcagtca tcaacaaaca catgcacaca tccatacaca cataatttaa 14101 aagaaagcga aagtaaccta taatgctaaa caaagcacat tctcagtgta gactgcagtg 14161 gtgatgaacc ttgaaaatat attaatagaa gccagacacg taagtcacaa atggtataac 14221 tcaatttata tgaaattccc agagagtcca aagttaaaaa aaacaaaaag gcagattatt 14281 ggttgtcagt agctacagag atgggacatt gaagatatta ataggtatag ggttgaggat 14341 tcatggcctt tccctcttcc atgttagcat gtctattgtt gttaacctta ttcaactcat 14401 gttagggcaa tcatattgat gagacttaat gagtatagct tgacattcct agaagacaga 14461 gtctcacatc agactctctg atcctctgac tcttagaatc tttcaacacc ctcttctgaa 14521 atgatctctg agcctttggt tgtgactgaa ttatgagaaa gaattttatg ttgaatgaat 14581 tttacttcag catttttaca aaactgaagc tggggatggc gtttcatgcg tgcagtccct 14641 accatttgcc tataagcctg gaggaggcag aagtagaatt aggcttttca gggacatctt 14701 cagctgcatc atgagcttga ggacaacttg agctacataa gattcccatc cccaccccca 14761 cacacacaca cacatctagt atgtacctag tatgcagtct tttcatatgt ttaaaatgta 14821 gaaaggtata tgtgaagtct cccttttccc cacaacttag tccctccaaa gtattgcatc 14881 agtttccttg tacatgattt tatgcatata taagatacac tatttctctc tctctctctc 14941 tccttccctc cttccctcca acctctgggt atgagttgag ggttatgtat gtttcaagtt 15001 gtaataagaa ttgccactca aacatttcat cccattttaa gttaagtttg tctgattttc 15061 cactttagaa gggcagccat gatggctcat gcctataatc ccatcctagc acttgtaagc 15121 ctaatacata agactggagg attgtcatca gcttgaggct agcctggtct acctaatgag 15181 ttccagccag ccaagagtag aatataagac cctgtctcca aaaaatgtac caaaggggct 15241 ggaggggtaa ctcagtggtt aagaacagta gctgctcttc cagaggacct gggttcaatt 15301 cccagcaccc acatggcagc ttgcaactat ctgtaactgt ccagctttgg gatatccgac 15361 accattatac caattacata aaatgaaaaa attgtaaaat gtgctaaaat gagtggggag 15421 ggggagcatg gtgtggttct gtgggcttgg gattttgcca tgaatccatg actatatctc 15481 agcaatatgt ggattatttc tggaaacctg tggggcatgg gtgtatggga agaggggaga 15541 gagagagagc ccgcatccag ccagagttcc tgtgctctgg gcaggcggac gtgggaggac 15601 tgctggacga tttccacttg gccctgggtg ggcatctggc tgtgtgaagc cactgacccc 15661 actcagcggg gggtagataa ggggcatttc ccataccagg ttctcagact ccagcattcc 15721 acgctggata cagcagaaga gctgagaaca gaataggggc cccagggaga cactctcgtc 15781 ccagagataa agaaggcaga gggagagaga ttcctacgca ggcgagaggc cttagtctgg 15841 gccttgatga acagagacgg tctatggttt tagagcttta ttgtagaaag gcagggggaa 15901 agagagaagg tagaaagaga gagaccagcc atggccaaga ggagagaagg ggggaaagga 15961 gagagagaag aaaggctaga gagtaagaaa ggttaaagag acagaagtaa aagcttagag 16021 taagagagag agagagagca agagggagag taagaaaagt aaaagcttaa gagagcgagg 16081 aggggccagc ccctctaata gtgggcttgt tatcttgttg ttgctaggta actgtgaagc 16141 gtctagcctg aaggccagaa gcttgggaca ttgtctatgt gactgaaagc catgagcctc 16201 tactgtgggg gctgtggagg tggtaacttg gacaggagcc aggggttcca ggagacatga 16261 gggaatgcct tctgtcccat gtaggtagga attaccacca ccggctttca ccgtagttca 16321 ctggagactg tttgtacata gcccattgcc ccacagaaac caggcactca ctgtttcatt 16381 tgcataagaa ttagaaggct ttattttggc tgggattggt gacttgcccc agaaattcca 16441 gcttttggga agccgaatcc tggagaacca ggagttcaag gtcatcctca ggtacctcat 16501 ttgttcaaga ccagcctgtg ctaccatgag accttgtccc tactcccctc aaccccaaaa 16561 cacgctgttt tcattttata ggttgtacta accatggagg atggtcattt gcctttctct 16621 tgatttcaga agcgtagcgc gggctttgca ccatgatcta gtgtttccag ataggcttgc 16681 ctctccacag ccaggcttat cctgcttcag tcccatgttt ggcaagggca acatatgact 16741 gaagaacttg catagcctta tggtgtatca tgactcttag ccccagttta taactgtcta 16801 acagctggtg gcttttgtga aaaaggaaag catcatttga acttttaact tttcagctgt 16861 ttgttttgac tttgcataaa tactgagact agctaggaaa tgagcttttg tttcatttgc 16921 taaaaatgat ttttttttca tgaggcaatt tcaagcaatt tgaatttagc aatctctact 16981 tgtctgaaaa gaagttatta attaaaaaaa aacaaacaaa caggaaccta ttatgctggc 17041 tagtcacatg aaagaatcca aatgaacaca gtcaaggctg aatcaacaca tgaaattatt 17101 ttctaattcc ccaaattaca tttatgctaa ttagtatgta gtaaaacatt ttaattgttt 17161 ccaatgttaa acattacaga aataattttg atgttttcct aataacccag cctttgatca 17221 tgtttgcatt tatttgattg ttgtcagttt attaaataat taagtaagag caacaagata 17281 aaatgacttc atgagagtcc ttttctgtaa cttagaacgt cagtagtcat aggaactgcg 17341 gtcgttccct tacctttcag aagtaggcta aagagttgaa cgcaaaggtg gtttttcgaa 17401 tcttcctgat ccttaagccc ccactaggtt tatattctat atttgatatc ggattcgaac 17461 ccctcgagct taggatccgg aacccttaat ataactaagt tattaggtcc ctcgaagagg 17521 ttcactagta ctggccattg cggccgcttc gaggctgcct cgcgcgtttc ggtgatgacg 17581 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 17641 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 17701 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 17761 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 17821 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 17881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 17941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 18001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 18061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 18121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 18181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 18241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 18301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 18361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 18421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 18481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 18541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 18601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 18661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 18721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 18781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 18841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 18901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 18961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 19021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 19081 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 19141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 19201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 19261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 19321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 19381 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 19441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 19501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 19561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 19621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 19681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 19741 tccgcgcaca tttccccgaa aagtgccacc tgacgg //