LOCUS       Exported                1049 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Mammalian expression of dmBACCS2VL, a modified Drosophila 
            melanogaster blue light-activated Ca2+ channel switch, along with 
            dOrai and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    dmBACCS2VL-IRES-dOrai-IRES-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      39..48
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcctcgaggc caccatggga ccggtcggag
       61 gaagcggtgg atctttggct actacacttg aacgtattga gaagaacttt ctcattactg
      121 acccaagatt gccagataat cccattatat tcgcgtccga tagtttcttg cagttgacag
      181 aatatagccg tgaagaaatt ttgggaagaa actgcaggtt tctacaaggt cctgaaactg
      241 atcgcgcgac agtgagaaaa attagagatg ccatagataa ccaaacagag gtcactgttc
      301 agctgattaa ttatacaaag agtggtaaaa agttctggaa cctctttcac ttgcagccta
      361 tgcgagatca gaagggagat gtccagtact ttattggggt tcagttggat ggaactgagc
      421 atgtccgaga tgctgccgag agagagggag tcatgctgat taagaaaact gcagaaaatc
      481 tgcaatcatg gcttcaatac acatatgaac tagaaagtaa gaatcatcag aagaagcgca
      541 cgtcggctga gaagcagcta cagtcggcca gagaggcttg tgagaaattg cgtaagaaac
      601 ggtcaagttt ggtgggtgcg ttcgtttcca cgcacggaaa gagtattgat gatgtggatc
      661 ggtcgattgt tgaggcacgg aatgccctcg gagatgtaac aaacgagctg caagaacgac
      721 tgcatcgctg gaagcaaatc gagacgtgcc ttggcttaaa cattgtgaac aacaatggtc
      781 tgcccggagg atccggagga agtggaggag gtatcctgca gtctcggggt ggcagtggtg
      841 gttcaggcgg ttctggatct ttggctacta cacttgaacg tattgagaag aactttctca
      901 ttactgaccc aagattgcca gataatccca ttatattcgc gtccgatagt ttcttgcagt
      961 tgacagaata tagccgtgaa gaaattttgg gaagaaactg caggtttcta caaggtcctg
     1021 aaactgatcg cgcgacagtg agaaaaatt
//
