LOCUS Exported 18664 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter. ACCESSION . VERSION . KEYWORDS pEMS1401 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 18664) AUTHORS Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM TITLE Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. JOURNAL Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. PUBMED 30765420 REFERENCE 2 (bases 1 to 18664) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..18664 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" regulatory 2398..2407 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2404..3120 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2404..3120 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2449..2470) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2710..2729) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3057..3078 /label=EGFP-C /note="EGFP, forward primer" CDS 3133..3150 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" CDS 3163..3183 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3187..3207 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3211..3231 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 3238..3271 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 3394..3475 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3431..3450) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11120..11141 /label=F1ori-F /note="F1 origin, forward primer" promoter 11304..11408 /gene="bla" /label=AmpR promoter CDS 11409..12269 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(16476..16498) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 16584..16724 /label=bom /note="basis of mobility region from pBR322" primer_bind 16639..16658 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(16739..16756) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(16910..17498) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(16990..17009) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(17669..18529) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(18530..18634) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac gcgtggcgcg ccggagaaga tcaagactca ggtgggtgga 181 gatgactgcc ccccatgcac acatttcctg gtgctccacc ccctcagcaa atgacttctg 241 ggtaagactg agggataaag cctatactgt tgatccttgc ccagctcaca gctgggatat 301 ttgggtagac ctcactagga ccctctcctt tacaccttag cctcccactg agagcattaa 361 gtgggcgaat tttgatgcct cccagctttc ccagctctta cctcctgccc ctggctattt 421 tctgttctta cctcctgctc cttgttttct gtccatagtg ggtgggggaa gcagaaagag 481 gaatgagaag gagagggatt gggatcttga ccccaacttc aaggccctca ccttctgtgg 541 agccctgagt ttggtccaca gggaacattc caacctctag tcattgctcc caccagattc 601 caggtccaca tcctgaaggc ttcatccctg tcccactagg atcctcccag gccctgggat 661 ccctcttttt ccctccacac tctgcctccc actcttctcc ctggctcctt tgcaccctcc 721 acgagcaaag atgaacactc cagaagttgc agcaaatgag ggtgaacatg ggccctgtag 781 aaccccagaa gtaggagttt ccagccatcc tcaatcaggc atcccaggac caggcactta 841 gcaggagacc tgcccattcc caggcctcct ccacccctgg gctcaagcag tgagagagga 901 ctttgagaat gccagttttg acagtacccg ttccatactg caaggctgca gaaggtgggc 961 ttctgggctg gagcctcatc ttcagccagc atcctcctta gcctgcctct ccagagccac 1021 gaagccccat aggtacccag agagactcca ggccacttgg gaaccacatc aacatctttg 1081 catgagttag aaataggaat ccttttcttg gagtggattc cagccttgta cagtgcacaa 1141 ccctgtctta acccctccac ctctccagcc tcgttcaggg atagccagaa tgggagtagt 1201 ggtgagggtg ggtggcagac tgggaggaca ggggatgggg aaggatggga gcagaatagt 1261 tgtacaggga agtagcaggg ggctcaggaa cccatcaggg aaagccagga cagattctca 1321 acatctttag aggccttcag cagtccaagc cactactaat ttatggggac tgaatgaatg 1381 agaaaaggag gggagggaga aggatgagag ggagagagaa ggctggaggg gagatgagag 1441 tcggagagaa aagcctagga ggtaaagaga gacctagaag ggggcgggga ggacagaaga 1501 gggggccacc catcataagt tgccaggaga gatggcccag caccaggcct gaggcttggg 1561 ggccccagaa aggcaggaaa cgggccaaag caagaaggag acggggaagg ggttcgggtc 1621 tctagggaag agcagagagt gggagaagag gaggagggag cggggatgcg ggcagagata 1681 aagggagcaa cggctgcagg gccgggcgcg cagcgacagg cgagagggag ggaagcggaa 1741 atttaaaagt gaagatgcag ataacgcagc ctggagacgg gaacccgggt ggggatagag 1801 ggagggaggg ggaggagagt gagcggcgag tggagcggga gccgactgga agaagggctc 1861 tagggagggg gctgtggctg ctggggtccg aggtggggcc gggtacacca gccccatcac 1921 tgtttgcaga gagtcaggga ggcggaaaag acacgcgctc taggctccca tcagggcaca 1981 tggcccggac ccatcccccg cgcgtctccc cggctgcggg gcgcgggggg ctgccgggtg 2041 cgcttggctg tggcgcggcg cgttggagac tttattgcga tgggacgata agaggggcgg 2101 gggcggggtc ctgggggccg aggcggcagc gctttaatta aaacggaaat tgcggccccg 2161 ggccgcgcgg gggccggagg gttccaagcg gccccttagc tggaagcgtt tctccaggac 2221 ccccccgcaa cccccgccac gcccgggctg ccccctcccg ccaggccctg ccggacccgg 2281 cgccgtcttc tcctccttgt cacccgcggt cgcttcgggc ggggatcggt gccaccgagc 2341 gcaaagcctg cctcggcgcg ccgaagttcc tattcttcaa aaggtatagg aacttccgcc 2401 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 2461 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 2521 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 2581 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 2641 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 2701 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 2761 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 2821 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 2881 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 2941 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 3001 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 3061 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 3121 ttatttctga actgctgccc gggctgctgc atggaaccgg atccaaaaaa gaagagaaag 3181 gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aatataagaa 3241 gttcctattc tctagaaagt ataggaactt cagcggccgc gactctagat cataatcagc 3301 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 3361 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 3421 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcgaatt 3481 ctcgtaaaac tcttcatggc agtagttatt attctctctc tctctctttt tcttttttct 3541 tgagacagga tatttctctg ttgcccaggc tggagtgcag tggcacagtc ttggctcact 3601 gcagcctgga cctcctgggc tcaagccatc ctcccacctc agcctcccaa gtagctgggg 3661 ctacaggcac atggccacca ggcccagata atttttcatt tttgtagaga ctgagtctca 3721 ccatgttacc caggtttatt attctcattt tttagatgaa gagactgagg tccagagaag 3781 ctcaatgact tgcctagttt tacaaatctc ctgccatcac atacccctca gcgtccttaa 3841 taagagggag gccaccaact atgtgctggg cactgtggtg gatgctggag ctatagggtt 3901 gagtatataa gaaatggtgt tgctggagca actgttgctt gcttacctga cctatctgag 3961 aattaattag caggggaaca tatttttgtt ttcagattca atataagaac ttgtgtgggc 4021 aaaaataaag atcagtagta ataacagtag ttcccatttg ctgactgtac tgtcctaagt 4081 gcatatatat atacatacac acacgcatac ctatactcct ctaatactca aaatgatcct 4141 gtttatgtat tgttaatatg ctcattttat ttttaaattt ttatttattt ttatttttat 4201 ttatttttga gacggagtct cattctgtcg cggaggctga agtgcagtgg tgcgatctca 4261 gctcagtgcg acctccgcct cccgggttca agtgattctc ctgcctcagc ctccggacta 4321 gctgggatta caggcgcccg cctccacgcc cagctaattt ttgtattttt agtagagatg 4381 gggtttcgcc atgttggcca ggctggtctc gtactcctga ccttgagtga tccacctgcc 4441 tcggcctccc aaagtgctgg gattacaggc atgagccacc gcgccgggct aatatgctca 4501 ttttagtgag gcaaaaatag aggctcagag tctgatttgt acaaaactac agagcagtta 4561 agtgtcctct cagatgtgta ccctgatctg ggtgactcta ggactctagg tctcaactgt 4621 tacaaccagt taagggtttg gggaagcact gggccaagag tcaggaaaat ggaagccaca 4681 ggtagtgcaa ggtcttggga atgggacgtc tggtccaagg attcacgcga tgactggaac 4741 ccgaagagcc ggggcccggt ttacggccgc catgaagcaa cgcgcgccgg taggtttggg 4801 aatcagggag ccctctgaat aggagactga gttgggaggg aaaggggctt cgctggggga 4861 gcctcggctt cttctgggag aaaattccca cggctaccta gtgagcctgc aaactggtag 4921 gcgccggcgt aggcgcgcgg gcggggccgg gggcggggcc tgcggggcgt ggcggggcgg 4981 gcagagggcg gggcctgctt ctcctcagct tcaggcggct gcgacgagcc ctcaggcgaa 5041 cctctcggct ttcccgcgcg gcgccgcctc ttgctgcgcc tccgcctcct cctctgctcc 5101 gccaccggct tcctcctcct gagcagtcag cccgcgcgcc ggccggctcc gttatggcga 5161 cccgcagccc tggcgtcgtg gtgagcagct cggcctgccg gccctggccg gttcaggccc 5221 acgcggcagg tggcggccgg gccctgaggc gcgggatcga tccatgacac ccccccgccc 5281 cccatatctc actataattc actgtcaaat ggcttttatg atttggagtt tggggatact 5341 tttaaagaca gggtcttggt agctcctaga tggcttggga ttcacttagt aacccttgct 5401 ggcctaaaat tcaggaactt ctacctcatc ttcccaagta ctgggttagt agggatgtcc 5461 agcatattta gcttgcttta atgattttgc tgtacttttt tctgtgtgta caggtgcatg 5521 tatatttata aaactaacat aatatatttt acccccaaag taccataacc ctaattaatg 5581 ctattaaatg ttttgtatgc tgggctctgg gcatactaat ataaccaata ttatacaatg 5641 caaaacaatt ttggaatatc ttctgtaaaa ttatcttatt agtaagttgt aaccatctca 5701 gacttctaag tttgagtttg taaaggatca gttttttttg gtgacctaca cacacacaca 5761 cacacacaca cacacacaca cacacagggg ggtggattct gagaatttag agaatttaga 5821 tggttaatac cagtttttat ttttattctg tgtgatattt atttattaag atttatttat 5881 ttattacatg taggtacact gtatctgtct tcagacacac cagaagaggg catcagatct 5941 cgttacagat ggttgtgagt caccatgtgg ttgctgggat ttgaactcag gacctctgga 6001 agaacagtca gtgctcttaa ccgttgagcc atctcaccag ccccgtgata ttttatttaa 6061 cttactagaa tatactgtgt taaaacatat tttaggggat tggggagatg gcccattctg 6121 taaagtgtct gccatgtcat cttcaggacc tgcgtttgta accccagcat ggagaagaca 6181 gagacaggga gacccctggg cttcctggca agctagacta gcaaatttgg ttaactaggt 6241 tcaatgagag actcagtctc aaaacataag aagtgaattt tttagaccca atttcaatct 6301 ctgaactcca tacgtatgca catgtacctg catataaata tatatacaca cccataaaca 6361 catgcacaca caaaaaatgg ttcaaaagtt cttaaaatgt ataagagtag aaagtgtaga 6421 gccgggagtg gtggcgcacg cctttaatcc cagcacttgg gagcagaggc aggtggattt 6481 ctgagttcga ggccagcctg gtctacagag tgagttccag gacagccaga gctatacaga 6541 gaaatcctgc ctcaaaaaac caaaaataaa gtgtatatac taatctttca tgcctaaaat 6601 atttattttt gccacatttt tcatacctct ttaatatttt tctttttctc tttgtgcttc 6661 aaaggaaatt ttgtgtcatg ttttactttt cattcttgac tctattcagc agtaagacgc 6721 agcatggctt tcttagagaa gtataatcta ttacaaaacg gtaacattta actgctctac 6781 ataccctctt gtagctaaat tcctctgtta aactaagact tattatctaa acgtaattaa 6841 atgttgatat cttataatgc tccactttga aacagctgtc tttcttttgc agattagcga 6901 tgatgaacca ggttatgacc tagatttgtt ttgtatacct aatcattatg ccgaggattt 6961 ggaaaaagtg tttattcctc atggactgat tatggacagg ttagtaagac ctcgattgaa 7021 gttattttta gagggtgtgt gtgtggtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 7081 tgtgtaagag agagaggaga ggcatgtgtg atatatatgt gaagtgtatt gcatgtacat 7141 ggaatccaga gaaggacatt aggtgtccta ctctgttgct ctcttattcc cttgaatcag 7201 tgtctcttgc tgaacctggg gcatacatac tgatttggct aggctgcctg gctagcaaga 7261 cccagggatc tgcttgtctc tgccccccag tgctgacgtt acaagcaggt gcaaccacat 7321 ctggttttta tatgggtact gggggatctg aacttagggc ctgatgcgtg cccaagtgct 7381 ctcacccgcc aagccacctc tccagccaaa gggttttttt tcttgtatta attctaactt 7441 agcaactgtt gagtagttga tgtttgaata taagctaagc caacttatta gaataaatgt 7501 tttctatttt ttacttgtat gtgtgagtgc ctgcatgtat gtatgtgaag catatgtatt 7561 ccaggatgtc agaattgtga tggattccct ggaactggag ttacagacca ttgtgaacca 7621 ctgtgtatgg gtgctgggaa ctgaacttta ggtcctctgc aaaaacagta aggatcttaa 7681 ctgataaact atctttctag ccctaaggct ttctactttt aaagttcgtt tctctttctc 7741 tctctctctc tctctctctc tctctctctc cctctccctc cctcctcctc ctcctcctct 7801 ttcccccctc tcgcctcctc tttctctctc ccctccctct ctctctctca cacacacaca 7861 cagacagaga gaggagagag aaaggaagcc ttactaatta attacctagt tttagacata 7921 actagtggga atccatagta agtaaatcaa cattcttgaa gagaggttag aagaattggc 7981 tctcgttgtc catcgaggct gtataagatt attcttctgt tttctcagct taccatacat 8041 attttctacg tagacaatac caattcaaat acactgtacg taaagtatat gcgcacacac 8101 acacacacac acacacacac acacacacaa gttacatatt gtcatatagt actaaaattt 8161 atggactttt tcatattaat acatctagag ctatcatgtt ttttatcttt taaaaattac 8221 tttacggggc tacagagatg tctcagcagt taggagcact gactgctctt ccagaggtcc 8281 tgagttcaat tcccagcaac cacatggtgg ctcacaacca tctgtaatgg gatctgatcc 8341 actcttatgg tgtgtctgaa gacagtgaca gtgtactcat atacatgaaa taaatcttta 8401 aaaaattact ttaccatcag ggtatatata tatatatata tgtgtgtgtg tgtgtgtgtg 8461 tgtatgaagt atttgctatt tgagagggtc tcactgttta gctcagtctg gcttggaacg 8521 ttctgtatag cccagactgg cctcaactca gcagtccccc tgaaaggctc tcatatgtgg 8581 agattacagg tatgtgctac tgtgcccagc cctacatgtt actttgtttt tgaaataggg 8641 tatcaaatag cttagactgg cttctaagtc agtgtgtagc taaggatggt ctgagcttct 8701 gaacctgcta cttcaactcc tggtgtgcca tcatggccag tttgcacagt tgtgagagta 8761 gaactcagga cctcctatgt gttactagac atgttctcta ccagctgagc tacatcccaa 8821 ttcgcctaca tttactttta ttttttaaaa gatttattta tgtatacagc actctgcctg 8881 catgtacatc tgcatgccag aagagggcac cagatctcat catacatagt tgtgagccac 8941 catgtggttg ctggggattg aactcagggc ctctggaaga gcagtcagtg ctcttaacct 9001 ctgagccatc tctgtagccc cctacattta ttttaaagat tataggctat tctattcata 9061 gatgtgccat aactgattta accagatctt cattattttg tacttagtag agtatattag 9121 agggaatgaa tcttaaatac acattttgac acttgctcag ctttagacaa gtaaaaatca 9181 ctggtcaagg aaatggtgct tagtggatgc ttctgaactg tcaaattgtc ctccaacaag 9241 ttacatcatt ttatacttcc aacgttatgt gagagttcat ttttctttag gctcttctag 9301 tattttatat ttgagaaata aaatttaacc aaaatttgcc aatctagtaa gcttgttttt 9361 atttgtatct ttaaatttta tgatagatgt tatagtgtac tctcctctcc cctcccctcc 9421 cctctcctcc tcagcaggat cttgttatgt agccctaagg gatctaatac ttactctgta 9481 gtccaggctg tccttcagct catgagacac accatcatgc ttggcatgtt tcttgttctg 9541 aaaggtatct ggttttaatt ttggttgagc tggtgtgttg gtgtatatct gtaatctcag 9601 cacaggcaag tagagacaag gattatgatt acagtccagc ctgggctatt tagagggatt 9661 ctctttaatc tttcctcatg ccccaaaatc ttacctttgg tatatgaaaa atagtctcca 9721 cttctgcaaa atattgcttt atgaagtaag aattaattcc cttcatagag acaaggaatg 9781 tgtcctgtaa aagtttaatg tgtaagaagt atttgttata aaagataaat attcagaatc 9841 ttctttttaa ttcctgattt tatttctata ggactgaaag acttgctcga gatgtcatga 9901 aggagatggg aggccatcac attgtggccc tctgtgtgct caaggggggc tataagttct 9961 ttgctgacct gctggattac attaaagcac tgaatagaaa tagtgataga tccattccta 10021 tgactgtaga ttttatcaga ctgaagagct actgtgtaag tataattaac ttataattaa 10081 aaaaataggg ccattctagt tttatctata ttttttttaa acttgtgcaa actatgctac 10141 ataatcaact tacttgatta tgtaatatat atagtaatag ttggaattac tattattaca 10201 tatttatttt tctgtgctgg ggtagaaccc agggaactaa gtactgtaat aaccgagctt 10261 cattcatcct agtcattttt aatttttttt tttttttttg ttttttcgag acaaggtttc 10321 tctgtgtagc cctggctgtc ctggaactca ctctgtagac caggctggcc tcgaactcag 10381 aaatctgcct gcctctgcct cccagtgctg agattaatga cgtgcaccac cactgcctgg 10441 cattttttta aagttttaat ttgagaccct ttctcactaa cttgctcaca ctatcctcga 10501 actcattacg tgggctggtc tagaaatttc agtcaacttg cctcagcttc tcaagtagct 10561 gggattacag gcaggtgcta ccacttctgg ccatgatcat tgttgttgtt gctattatta 10621 ttattattat ttcactgata cttcatatca caacacagaa tagtaactgt agttcatgac 10681 caacaaaaaa gttgtacagt ttctctgttg tcttattttg atctggattt tgatttgttt 10741 catggcactg ggcaggctaa tctcaaactg catggctcaa atagtccttc tgtcttaatc 10801 tcccatgttc tggaagtata ggtatcacta cacctagctg tattttcata ttttaaaaaa 10861 aacgcctaag aattgctatt gaatgaggcg gagagatggc tcagcagtca caagtacttg 10921 tagaggactg gggtttggtt actagcacct caaagatggc ttacaattat ctgtagctcc 10981 aattccagag gatctgtgtg gtaaagctgc atgtatatgg gcatgcaggt aaaactgcta 11041 aacatataaa aatagctcag tcttaagaga attggtcgac tttttcgccc tttgacgttg 11101 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 11161 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 11221 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 11281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 11341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 11401 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 11461 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 11521 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 11581 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 11641 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 11701 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 11761 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 11821 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 11881 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 11941 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 12001 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 12061 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 12121 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 12181 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 12241 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 12301 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 12361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 12421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 12481 caaaaaaacc accgctacca ggtcgagtag ggataacagg gtaatggatc ctcgttgggc 12541 acctgcactc atgtacgcag gcccacatat agatgtacac acgcatatca ttcaaaatag 12601 aaattattta tacaagtagt gcgttggaga gctggctcag gagttgttaa tgcttgctac 12661 ccttgcaaag gacccaagtt caatttccag cacctgtgtt gggcatctca caaccacatg 12721 taaatctagt ttcaggggat ttcacccctc ttctgaactc ttcaggcact tatacccaca 12781 aatggacatc tccacataga gacacatact tttaaaaaag taatctaggg tctagagaaa 12841 tggttataga ccacggactg ggatcagttc ccagaagcca catcctgtgg ctcacaattg 12901 cctgtgaatc aagttctaga tctggtggcc tcgtctagcc tgcagtcatc aacaaacaca 12961 tgcacacatc catacacaca taatttaaaa gaaagcgaaa gtaacctata atgctaaaca 13021 aagcacattc tcagtgtaga ctgcagtggt gatgaacctt gaaaatatat taatagaagc 13081 cagacacgta agtcacaaat ggtataactc aatttatatg aaattcccag agagtccaaa 13141 gttaaaaaaa acaaaaaggc agattattgg ttgtcagtag ctacagagat gggacattga 13201 agatattaat aggtataggg ttgaggattc atggcctttc cctcttccat gttagcatgt 13261 ctattgttgt taaccttatt caactcatgt tagggcaatc atattgatga gacttaatga 13321 gtatagcttg acattcctag aagacagagt ctcacatcag actctctgat cctctgactc 13381 ttagaatctt tcaacaccct cttctgaaat gatctctgag cctttggttg tgactgaatt 13441 atgagaaaga attttatgtt gaatgaattt tacttcagca tttttacaaa actgaagctg 13501 gggatggcgt ttcatgcgtg cagtccctac catttgccta taagcctgga ggaggcagaa 13561 gtagaattag gcttttcagg gacatcttca gctgcatcat gagcttgagg acaacttgag 13621 ctacataaga ttcccatccc cacccccaca cacacacaca catctagtat gtacctagta 13681 tgcagtcttt tcatatgttt aaaatgtaga aaggtatatg tgaagtctcc cttttcccca 13741 caacttagtc cctccaaagt attgcatcag tttccttgta catgatttta tgcatatata 13801 agatacacta tttctctctc tctctctctc cttccctcct tccctccaac ctctgggtat 13861 gagttgaggg ttatgtatgt ttcaagttgt aataagaatt gccactcaaa catttcatcc 13921 cattttaagt taagtttgtc tgattttcca ctttagaagg gcagccatga tggctcatgc 13981 ctataatccc atcctagcac ttgtaagcct aatacataag actggaggat tgtcatcagc 14041 ttgaggctag cctggtctac ctaatgagtt ccagccagcc aagagtagaa tataagaccc 14101 tgtctccaaa aaatgtacca aaggggctgg aggggtaact cagtggttaa gaacagtagc 14161 tgctcttcca gaggacctgg gttcaattcc cagcacccac atggcagctt gcaactatct 14221 gtaactgtcc agctttggga tatccgacac cattatacca attacataaa atgaaaaaat 14281 tgtaaaatgt gctaaaatga gtggggaggg ggagcatggt gtggttctgt gggcttggga 14341 ttttgccatg aatccatgac tatatctcag caatatgtgg attatttctg gaaacctgtg 14401 gggcatgggt gtatgggaag aggggagaga gagagagccc gcatccagcc agagttcctg 14461 tgctctgggc aggcggacgt gggaggactg ctggacgatt tccacttggc cctgggtggg 14521 catctggctg tgtgaagcca ctgaccccac tcagcggggg gtagataagg ggcatttccc 14581 ataccaggtt ctcagactcc agcattccac gctggataca gcagaagagc tgagaacaga 14641 ataggggccc cagggagaca ctctcgtccc agagataaag aaggcagagg gagagagatt 14701 cctacgcagg cgagaggcct tagtctgggc cttgatgaac agagacggtc tatggtttta 14761 gagctttatt gtagaaaggc agggggaaag agagaaggta gaaagagaga gaccagccat 14821 ggccaagagg agagaagggg ggaaaggaga gagagaagaa aggctagaga gtaagaaagg 14881 ttaaagagac agaagtaaaa gcttagagta agagagagag agagagcaag agggagagta 14941 agaaaagtaa aagcttaaga gagcgaggag gggccagccc ctctaatagt gggcttgtta 15001 tcttgttgtt gctaggtaac tgtgaagcgt ctagcctgaa ggccagaagc ttgggacatt 15061 gtctatgtga ctgaaagcca tgagcctcta ctgtgggggc tgtggaggtg gtaacttgga 15121 caggagccag gggttccagg agacatgagg gaatgccttc tgtcccatgt aggtaggaat 15181 taccaccacc ggctttcacc gtagttcact ggagactgtt tgtacatagc ccattgcccc 15241 acagaaacca ggcactcact gtttcatttg cataagaatt agaaggcttt attttggctg 15301 ggattggtga cttgccccag aaattccagc ttttgggaag ccgaatcctg gagaaccagg 15361 agttcaaggt catcctcagg tacctcattt gttcaagacc agcctgtgct accatgagac 15421 cttgtcccta ctcccctcaa ccccaaaaca cgctgttttc attttatagg ttgtactaac 15481 catggaggat ggtcatttgc ctttctcttg atttcagaag cgtagcgcgg gctttgcacc 15541 atgatctagt gtttccagat aggcttgcct ctccacagcc aggcttatcc tgcttcagtc 15601 ccatgtttgg caagggcaac atatgactga agaacttgca tagccttatg gtgtatcatg 15661 actcttagcc ccagtttata actgtctaac agctggtggc ttttgtgaaa aaggaaagca 15721 tcatttgaac ttttaacttt tcagctgttt gttttgactt tgcataaata ctgagactag 15781 ctaggaaatg agcttttgtt tcatttgcta aaaatgattt ttttttcatg aggcaatttc 15841 aagcaatttg aatttagcaa tctctacttg tctgaaaaga agttattaat taaaaaaaaa 15901 caaacaaaca ggaacctatt atgctggcta gtcacatgaa agaatccaaa tgaacacagt 15961 caaggctgaa tcaacacatg aaattatttt ctaattcccc aaattacatt tatgctaatt 16021 agtatgtagt aaaacatttt aattgtttcc aatgttaaac attacagaaa taattttgat 16081 gttttcctaa taacccagcc tttgatcatg tttgcattta tttgattgtt gtcagtttat 16141 taaataatta agtaagagca acaagataaa atgacttcat gagagtcctt ttctgtaact 16201 tagaacgtca gtagtcatag gaactgcggt cgttccctta cctttcagaa gtaggctaaa 16261 gagttgaacg caaaggtggt ttttcgaatc ttcctgatcc ttaagccccc actaggttta 16321 tattctatat ttgatatcgg attcgaaccc ctcgagctta ggatccggaa cccttaatat 16381 aactaagtta ttaggtccct cgaagaggtt cactagtact ggccattgcg gccgcttcga 16441 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 16501 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 16561 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 16621 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 16681 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 16741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 16801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 16861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 16921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 16981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 17041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 17101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 17161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 17221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 17281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 17341 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 17401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 17461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 17521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 17581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 17641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 17701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 17761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 17821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 17881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 17941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 18001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 18061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 18121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 18181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 18241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 18301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 18361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 18421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 18481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 18541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 18601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 18661 acgg //