LOCUS       Exported                 779 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
            control of human synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-DIO-hM4D(Gi)-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, 
            Maratos-Flier E, Roth BL, Lowell BB
  TITLE     Rapid, reversible activation of AgRP neurons drives feeding behavior
            in mice.
  JOURNAL   J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
  PUBMED    21364278
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(255..288)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(339..372)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
ORIGIN
        1 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
       61 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      121 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      181 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      241 aagtaaggcg cgccataact tcgtataatg tatgctatac gaagttatta agaggtttca
      301 tattgctaat agcagctaca atccagctac cattctgcat aacttcgtat aaagtatcct
      361 atacgaagtt attccggagt cgactctaga ggatccggta ccttctcgac tgcgctctca
      421 ggcacgacac gactcctccg ctgcccaccg cagactgagg cagcgctgag tcgccggcgc
      481 cgcagcgcag atggtcgcgc ccgtgccccc ctatctcgcg cctcgcgtgg tgcggtccgg
      541 ctgggccggc ggcggcgcgg acgcgaccaa ggtggccggg aaggggagtt tgcgggggac
      601 cggcgagtga cgtcagcgcg ccttcagtgc tgaggcggcg gtggcgcgcg ccgccaggcg
      661 ggggcgaagg cactgtccgc ggtgctgaag ctggcagtgc gcacgcgcct cgccgcatcc
      721 tgtttcccct ccccctctct gataggggat gcgcaatttg gggaatgggg gttgggtgc
//
