LOCUS       Exported                 811 bp ds-DNA     linear   SYN 05-JUN-2021
DEFINITION  Lentiviral expression of shRNA against CRTC1 (3rd generation).
ACCESSION   .
VERSION     .
KEYWORDS    pLL3.7 shRNA against CRTC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Jeanneteau FD, Lambert WM, Ismaili N, Bath KG, Lee FS, Garabedian 
            MJ, Chao MV
  TITLE     BDNF and glucocorticoids regulate corticotrophin-releasing hormone 
            (CRH) homeostasis in the hypothalamus.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1305-10. doi: 
            10.1073/pnas.1114122109. Epub 2012 Jan 9.
  PUBMED    22232675
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 5, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      15..24
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(63..266)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(92..112)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        267..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(686..719)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(761..777)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(762..778)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tagcgctagc ggatctgacg gttcactaaa
       61 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      121 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      181 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
      241 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
      301 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
      361 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
      421 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
      481 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
      541 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
      601 gaccccgtaa ttgattacta ttaataacta atgcatggcg gtaatacggt tatccacgcg
      661 gccgcttaag cttggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
      721 aggtccctcg acctgctgga atctcgtgaa gcgagcttat cgataccgtc gacctcgaga
      781 attcaaaaac gaacaatccg cggaaattta a
//
