LOCUS       Exported                 794 bp ds-DNA     linear   SYN 10-JUL-2021
DEFINITION  Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycLAM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    136..205
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    234..368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(258..277)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     312..331
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(640..660)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgccgacgcc gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg
       61 cgagggcttc ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt
      121 catcatgatg gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc
      181 ctggcacccg caccgcggct tcgagaccgt gacctacatc gtcgacacta gtggatccag
      241 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      301 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      361 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      421 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
      481 caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc
      541 ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa
      601 aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg
      661 aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga
      721 gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata
      781 ggttggaatc taaa
//
