LOCUS Exported 9707 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.. ACCESSION . VERSION . KEYWORDS Cntn5.1-AP-His SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9707) AUTHORS Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM TITLE An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. JOURNAL Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149. PUBMED 26633812 REFERENCE 2 (bases 1 to 9707) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9707 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 105..408 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 409..612 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 563..583 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 609..633 /label=LNCX /note="Human CMV promoter, forward primer" intron 748..1573 /label=CMV intron A /note="human cytomegalovirus intron A (Chapman et al., 1991)" regulatory 1604..1613 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6287..6304 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 6330..6451 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6367..6386) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6421..6440 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6489..6506) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6660..7248) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6740..6759) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7419..8279) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8042..8061 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8280..8384) /gene="bla" /label=AmpR promoter primer_bind 8452..8470 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8508..8530) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8532..8552) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8537..8867 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8718..8853 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8780..8799 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 9004..9023 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(9090..9545) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9227..9248) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 9439..9458 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9671..9693 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 9685..9702 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 9686..9702 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa 61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt 301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac 361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga 721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc 781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc 841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac 901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca 961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta 1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc 1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt 1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc 1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac 1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt 1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta 1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg 1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg 1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg 1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcttcctg 1621 ttggagactc attctgttcc tgtctgtcac caggtggctt tcagattact ctgaagctct 1681 ctctggcctt tctacttcat atgctgcttt gctcagaatt aagaagagtt caacctcacc 1741 ctttggttcc aagtccagac cacggttcag cagtccttca ttaggtacca tcagtgtttc 1801 tccaccaagc tggcgagggg cagctcagca gtaccactcc ccaggcaacc tttatcactc 1861 ctcagatgcc ttcagacaag atgaaagtgt ggattatggg ccagtatttg tgcaagaacc 1921 tgatgatgtc atttttccaa ctgattctga tgaaaagaag gtagctctaa attgtgaagt 1981 tcgtggcaac ccatctccta gttacagatg gcttcggaat ggaactgaaa tagctctgga 2041 gagtgactat cgctacagtc tgatagacgg caccttcatc atcagcaatc caagtgaact 2101 cagggattct ggcctgtacc agtgcttagc caccaactct tttggaagta ttcttagtcg 2161 agaagccact cttcagtttg cctatctggg aaattttagt ggccggacaa gaagtgcagt 2221 ctcagtgcgg gaaggccagg gggttgttct gatgtgctct ccaccaccac attctccaga 2281 gatcatctat agctgggtct tcaatgagtt cccttccttt gtggcagagg acagccggcg 2341 gttcatatcc caagagacag gcaaccttta tatttctaaa gtgcaaacat ccgatgttgg 2401 cagctacatt tgtctggtga aaaatgctgt gacaaatgct cgcgtgctca gtcctccaac 2461 cccactcacg ctgcgaaatg atggtgtgat gggagaatat gagccaaaaa ttgaggtcca 2521 ttttcctttc actgtcacag ctgctaaggg aacaactgtg aagatggaat gctttgcact 2581 tggcaacccg gttccaacaa ttacttggat gaaggttaac ggttacattc ctagtaagtc 2641 cagactgcgg aaatcccagg cagtgctgga gattccaaac ctgcagctgg atgatgccgg 2701 aatatacgaa tgcacagctg aaaactcccg tggaaaaaac tcctttcgtg gacagctaca 2761 aatattcaca tacccacact gggtacagaa actgaatgat acccagctgg acagtgggag 2821 tcctctgcag tgggaatgca aggctactgg aaagcccaga cccacatacc gctggctgaa 2881 gaatggagca cccctcttgc cgcagagcag agtggacaca gtgaatggaa tattggcgat 2941 ccagagtgtg aatcaatcag atgctgggat gtatcagtgt ttggctgaga ataaatatgg 3001 agctatttat gcgagtgctg agctgaagat tctagcttcg ccgccctcct tcgaactgaa 3061 tcaagtgaag aaatccataa ttgttaccaa agaccgggga gtgctcatag agtgcgaacc 3121 ccagggatct ccaaagccag ccatctcctg gaggaaggga gacaaggcag tgagggcgaa 3181 taaaagaata gctattcttc cagacggaag cctgaggatc ctaaacgctt ctaaagcaga 3241 tgaggggaag tacatttgcc aaggggtgaa catcttcggt tctgcagaga ttatagcttc 3301 cctgtctgtc aaagaaccca caaggataga gcttactcct aaaagaacag agttaactgt 3361 tggagagagc attgtcctca actgtaaagc tatccacgac gccagtttgg acgtcacttt 3421 ctactggaca ttaaaaggac aacctattga ttttgagaaa gaaggcggac attttgaaaa 3481 catcagggct caagcatcct ctgcagattt aatgatcagg aacatccttc tgatgcatgc 3541 tgggagatat ggctgcaggg tacagaccac agcagacagt gtgtcagatg aggcagaact 3601 tcttgttagg ggtcccccag ggccacctgg agtggtcata gtagaggaga ttacagaaag 3661 cacagccact ctctcatgga gtccagcaac tgacaaccat agccccatct cttcttacaa 3721 ccttcaggct cgcagcccct tctccctggg ctggcaaaca gtgaagacag tcccagaagt 3781 catcacagga gacatggagt cagcaatggc tgtggaccta aacccctggg tagaatatga 3841 attccgagtg gtggccacca atccaattgg gacaggagat ccaagcattc catctcgaat 3901 gatccgcaca aatgaggcag ttccaaagac ggcacccagc aacgtgagtg gaagaagtgg 3961 aagaaggcat gagttggtca ttgcctggga gccagtatca gaagagtttc agaatgggga 4021 aggcttcggc tacatagtgg cattcagacc taatggaaca cgtggctgga aggagaaaat 4081 ggttacgtct tcggaggcgt ccaagttcat ttaccgagat gaaagtgtcc ctcctctcac 4141 accctttgag gtcaaagttg gtgtttataa taataaaggt gatgggcctt tcagccagat 4201 cgtagtcatc tgttcagctg agggagaacc cactgctgct cccactgacg tcacagcaac 4261 cagtgtgtca gtgtcagaga tttttgttgt atggaaacat gttaaggaga gtcttggaag 4321 accacagggg tttgagatta gttactggaa agataccgag ccagaagact ccgtagaaac 4381 agtccgaacc agagggaatg agtcattcgt catgttgacg ggattggaag gcaacacact 4441 ctatcacctc acagtcaggg cttacaatgg agccggctat gggccaccca gcagggaagc 4501 aagcaccacc acaaagaggc acccccctag ggagccacct ggcaacctcc ggtgggagca 4561 gcagggctcc caagtttccc taggctggga gccagtcaga cccctagcca atgagtctga 4621 agtcatgggt tacaaggttt tttataggca agagggtcac agcgaaggtc aagttattga 4681 gacacagaaa cctcaagctg tagtgcctct acctgaggct ggagtctaca ttattgaagt 4741 tcgagcgtac agtgagggag gagatggaac agctagctcc caaatcaggg ttccttcata 4801 tactagtgga tcttccggaa tcatcccagt tgaggaggag aacccggact tctggaaccg 4861 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa 4921 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat 4981 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt 5041 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc 5101 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc 5161 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa 5221 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc 5281 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt 5341 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat 5401 ggacattgac gtgatcctag gtgggggccg aaagtacatg tttcgcatgg gaaccccaga 5461 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt 5521 gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat 5581 gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat 5641 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc 5701 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg 5761 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt 5821 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt 5881 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat 5941 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg 6001 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag 6061 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg 6121 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga 6181 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg 6241 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtcatc atcaccatca 6301 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca 6361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 6421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct 6481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 7021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 7081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 7141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 7201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 7261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 7321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 7381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 7441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 7501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 7561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 7621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 7681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 7741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 7801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 7861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 8041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 8101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 8161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 8221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 8281 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 8341 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 8401 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 8461 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 8521 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag 8581 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 8641 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 8701 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 8761 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 8821 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 8881 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 8941 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 9001 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 9061 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt 9121 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 9181 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 9241 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 9301 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 9361 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 9421 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 9481 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 9541 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 9601 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 9661 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt //