LOCUS Exported 10260 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.. ACCESSION . VERSION . KEYWORDS lentiGuide-Crimson SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10260) AUTHORS Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE TITLE BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. JOURNAL Nature. 2015 Sep 16. doi: 10.1038/nature15521. PUBMED 26375006 REFERENCE 2 (bases 1 to 10260) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10260 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 87..1264 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 318..1255 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1212..1232 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 1271..1280 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1277..1954 /codon_start=1 /product="far-red noncytotoxic tetrameric variant of DsRed fluorescent protein (Strack et al., 2009)" /label=E2-Crimson /note="mammalian codon-optimized" /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH LFQ" primer_bind 1675..1694 /label=mCherry-F /note="mCherry, forward primer" misc_feature 1970..2558 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2023..2043) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2441..2452) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 2630..2863 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 2941..3062 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2978..2997) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3032..3051 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3102..3237 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3164..3183 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3257..3276) /label=T7 /note="T7 promoter, forward primer" promoter complement(3258..3276) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3286..3303) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3286..3302) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3295..3317) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3444..3899 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3531..3550) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3741..3762 /label=F1ori-F /note="F1 origin, forward primer" promoter 3925..4029 /gene="bla" /label=AmpR promoter CDS 4030..4890 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4248..4267) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5061..5649 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5550..5569 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5803..5820 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5937..5958 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5973..6003 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6011..6027 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6016..6038 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6035..6051 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6035..6051 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6070..6090 /label=T3 /note="T3 promoter, forward primer" promoter 6072..6090 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6118..6344 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 6345..6525 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 6572..6697 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7190..7423 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7608..7652 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 7830..8070 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 7830..7850 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 8001..8020 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 9960..10035 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 10091..10208 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc 421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc 481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg 541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg 601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca 661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac 721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca 781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc 841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc 901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc 961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta 1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg 1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt 1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc 1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc 1261 gtgacgtacg gccaccatgg atagcactga gaacgtcatc aagcccttca tgcgcttcaa 1321 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg tgggcgaggg 1381 caagccctac gagggcaccc agaccgccaa gctgcaagtg accaagggcg gccccctgcc 1441 cttcgcctgg gacatcctgt ccccccagtt cttctacggc tccaaggcgt acatcaagca 1501 ccccgccgac atccccgact acctcaagca gtccttcccc gagggcttca agtgggagcg 1561 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 1621 cggcaccctc atctaccacg tgaagttcat cggcgtgaac ttcccctccg acggccccgt 1681 aatgcagaag aagactctgg gctgggagcc ctccactgag cgcaactacc cccgcgacgg 1741 cgtgctgaag ggcgagaacc acatggcgct gaagctgaag ggcggcggcc actacctgtg 1801 tgagttcaag tccatctaca tggccaagaa gcccgtgaag ctgcccggct accactacgt 1861 ggactacaag ctcgacatca cctcccacaa cgaggactac accgtggtgg agcagtacga 1921 gcgcgccgag gcccgccacc acctgttcca gtagacgcgt taagtcgaca atcaacctct 1981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 2041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 2101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 2161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 2221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 2281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 2341 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 2401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 2461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 2521 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact 2581 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 2641 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 2701 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 2761 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 2821 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca 2881 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 2941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 3001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 3061 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 3121 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 3181 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 3241 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 3301 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 3361 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 3421 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 3481 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 3541 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 3601 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 3661 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 3721 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 3781 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 3841 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 3901 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 3961 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 4021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 4081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 4141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 4201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 4261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 4321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 4381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 4441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 4501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 4561 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 4621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 4681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 4741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 4801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 4861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 4921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 4981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 5221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 5761 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 5821 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 5881 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 5941 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 6001 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 6061 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt 6121 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct 6181 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg 6241 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc 6301 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag 6361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 6541 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 6601 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 6661 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 6721 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 6781 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 6841 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 6901 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 6961 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 7021 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 7081 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 7141 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 7201 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 7261 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 7321 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 7381 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 7441 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 7501 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 7561 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 7621 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 7681 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 7741 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 7801 cccacctccc aaccccgagg ggacccagag agggcctatt tcccatgatt ccttcatatt 7861 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa 7921 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt 7981 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt 8041 tcttggcttt atatatcttg tggaaaggac gaaacaccgg agacggttgt aaatgagcac 8101 acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt 8161 ctttttaata actttagtat caataattag aatttttatg ttcctttttg caaactttta 8221 ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct 8281 ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt 8341 tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca 8401 actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat 8461 gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat gatttcttct 8521 tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc tgcttttttt 8581 acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc accaatattt 8641 aataccatat caatttctgt tgcaccatct ttaattgctt cagaaacttc gaatgctttt 8701 gtagctgttg tgcatgcacc tagaggaaaa cctacaacat ttgttattcc tacatttgtg 8761 ccttttaata attctttaca atagcttgtt caatatgaat taacacaaac tgttgcaaaa 8821 tcaaattcaa ttgcttcatc acataattgt ttaatttcag ctttcgtagc atcttgtttt 8881 aataatgtgt gatctatata tttgtttagt ttcatttttt ctcctatata ttcattttta 8941 attttaattc tttaataatt tcgtctactt taactttagc gttttgaaca gattcaccaa 9001 cacctataaa ataaattttt agtttaggtt cagttccact tgggcgaaca gcaaatcatg 9061 acttatcttc taaataaaat tttagtaagt cttgtcctgg catattatac attccatcga 9121 tgtagtcttc aacattaaca actttaagtc cagcaatttg agttaagggt gttgctctca 9181 atgatttcat taatggttca atttttaatt tcttttcttc tggtttaaaa ttcaagttta 9241 aagtgaaagt gtaatatgca cccatttctt taaataaatc ttctaaatag tctactaatg 9301 ttttattttg ttttttataa aatcaagcag cctctgctat taatatagaa gcttgtattc 9361 catctttatc tctagctgag tcatcaatta catatccata actttcttca taagcaaaaa 9421 caaaatttaa tccgttatct tcttctttag caatttctct acccattcat ttaaatccag 9481 ttaaagtttt tacaatatta actccatatt tttcatgagc gattctatca cccaaatcac 9541 ttgttacaaa acttgaatat agagccggat tttttggaat gctatttaag cgttttagat 9601 ttgataattt tcaatcaatt aaaattggtc ctgtttgatt tccatctaat cttacaaaat 9661 gaccatcatg ttttattgcc attccaaatc tgtcagcatc tgggtcattc ataataataa 9721 tatctgcatc atgtttaata ccatattcaa gcggtatttt tcatgcagga tcaaattctg 9781 gatttggatt tacaacattt ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa 9841 taacgttata tcctgattca cgtaatattt ttggggtaaa tttagttcct gttccattaa 9901 ctgcgctaaa aataattttt aaatcttttt tagcttcttg ctcttttttg tacgtctctg 9961 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg 10021 gcaccgagtc ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat 10081 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 10141 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 10201 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg //